Plant Cell, Tissue and Organ Culture

, Volume 83, Issue 3, pp 251–257 | Cite as

Manipulation of the culture environment on in vitro air movement and its impact on plantlets photosynthesis

  • Yoshiaki Kitaya
  • Yoshitaka Ohmura
  • Chieri Kubota
  • Toyoki Kozai
Article

Abstract

Two-dimensional air current speeds in the culture vessel were measured using a tracer-based visualization technique and the effect of the air movement in the culture vessel on the photosynthesis of in vitro potato plantlets was assessed under a photoautotrophic culture condition. The air current speeds inside the vessel were varied by controlling free convection induced by spatial variations of temperatures in the culture vessel. For all conditions examined, upward air currents were observed around the plantlets in the central part of the culture vessel and downward air currents were observed near inside walls in the culture vessel. The upward and downward air currents were restricted by the presence of the plantlet. The upward air current speeds were affected by plantlet size inside the vessel and it was 24, 8 and 4 mm s−1 in culture vessels with no plantlets, a 10-mm-tall plantlet and a 60-mm-tall plantlet cultured inside the vessel, respectively. The upward air current speed was increased by 2 times by increasing wind velocity above the culture vessel from 0.1 to 1.0 m s−1. Placing the black plate on the medium also increased the air current speeds by 1.5 times. The net photosynthetic rates of the plantlets increased from 2.0 to 2.5 μmol m−2 s−1 as the upward air current speed in the culture vessel increased from 2.4 to 8.0 mm s−1. The air current speeds in the culture vessel were significantly slow. Enhancement of the air movement in the culture vessel is important to promote photosynthesis of the in vitro plantlets.

Keywords

air movement micropropagation photosynthesis plant tissue culture potato 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Daws, LF, Penwarden, AD, Waters, GT 1965A visualization technique for the study of air movement in roomsJ. Instn. Heat. Vent. Engs.332430Google Scholar
  2. De, Y, Desjardins, Y, Gosselin, A 1993aPhotosynthetic capacity and effects of forced ventilation on growth of in vitro-cultured strawberry plantletsActa Hortic. Sinica20123126Google Scholar
  3. De, Y, Gosselin, A, Desjardins, Y 1993bEffects of forced ventilation at different relative humidities on growth, photosynthesis and transpiration of geranium plantlets in vitroCan. J. Plant Sci.73249256Google Scholar
  4. Kitaya, Y, Fukuda, T, Kozai, T, Kirdmanee, C 1995Effects of light intensity and lighting direction on the photoautotrophic growth and morphology of potato plantlets in vitroScientia Hortic.621524CrossRefGoogle Scholar
  5. Kitaya, Y, Ohmura, Y, Kozai, T 1997Method for visualization of air currents in plant tissue culture vesselsEnviron. Control Biol.35139141(in Japanese with English summary)Google Scholar
  6. Kitaya, Y, Tsuruyama, J, Kawai, M, Shibuya, T, Kiyota, M 2000

    Effects of air current on transpiration and net photosynthetic rates of plants in a closed plant production

    Kubota, CChun, C eds. Transplant Production in the 21st CenturyKluwer Academic PublishersDordrecht, Netherland8390
    Google Scholar
  7. Kitaya, Y, Kawai, M, Tsuruyama, J, Takahashi, H, Tani, A, Goto, E, Saito, T, Kiyota, M 2001The effect of gravity on surface temperature and net photosynthetic rate of plant leavesAdv. Space Res.28659664CrossRefPubMedGoogle Scholar
  8. Kitaya, Y, Kawai, M, Tsuruyama, J, Shibuya, T, Kiyota, M 2003aEffects on air current speed on the transpiration rate and net photosynthetic rate of plant leavesAdv. Space Res.31177182CrossRefGoogle Scholar
  9. Kitaya, Y, Kawai, M, Tsuruyama, J, Takahashi, H, Tani, A, Goto, E, Saito, T, Kiyota, M 2003bThe effects of gravity on surface temperatures of plant leavesPlant, Cell and Environment26497503Google Scholar
  10. Kozai, T 1991

    Autotrophic micropropagation

    Bajaj, YPS eds. Biotechnology in Agriculture and ForestrySpringer-VerlagN.Y.313343
    Google Scholar
  11. Kozai, T, Ohde, N, Kubota, C 1991Similarity of growth patterns between plantlets and seedlings of Brassica campestris L. under different in vitro environmental conditionsPlant Cell Tiss. Org. Cult.2418186CrossRefGoogle Scholar
  12. Kozai, T, Fujiwara, K, Hayashi, M, Aitken-Christie, J 1992

    The in vitro environment and its control in micropropagation

    Kurata, KKozai, T eds. Transplant Production SystemsKluwer Academic PublishersDordrecht247282
    Google Scholar
  13. Kozai, T, Smith, MAL 1994

    Environmental control in plant tissue culture

    Aitken-Christie, JKozai, TSmith, MAL eds. Automation and Environmental Control in Plant Tissue CultureKluwer Academic PublishersDordrecht301318
    Google Scholar
  14. Kubota, C, Kozai, T 1992Growth and net photosynthetic rate of Solanum tuberosum in vitro under forced and natural ventilationHortScience2713121314Google Scholar
  15. Monteith, JL, Unsworth, MH 1990Principles of Environmental PhysicsEdward and Arnold Publishing CoLondon101120Google Scholar
  16. Murashige, T, Skoog, F 1962A revised medium for rapid growth and bioassay with tabacco tissue culturesPhysiol. Plant.15473497Google Scholar
  17. Nakayama, M, Kozai, T, Watanabe, K 1991Effect of the presence/absence of the sugar in the medium and natural forced ventilation on the net photosynthetic rates of potato explants in vitroPlant Tiss. Cult. Lett.8105109(in Japanese with English summary)Google Scholar
  18. Nguyen, QT, Kozai, T, Heo, J, Thai, DX 2001Photoautotrophic growth response of in vitro cultured coffee plantlets to ventilation methods and photosynthetic photon fluxes under carbon dioxide enriched conditionsPlant Cell Tiss. Org. Cult.66217225CrossRefGoogle Scholar
  19. Ohyama, K, Kozai, T 1997CO2 concentration profiles in a plant tissue culture vesselEnviron. Contr. Biol.35197202(in Japanese with English summary)Google Scholar
  20. Torrance, KE, Orloff, L, Rockett, JA 1969Experiments on natural convection enclosures with localized heating from belowJ. Fluid Mech.362131Google Scholar
  21. Yabuki, K, Miyagawa, H 1970Studies on the effect of wind speed upon the photosynthesis (2)Japan. J. Agric. Met.26137141(in Japanese with English summary)Google Scholar
  22. Zobayed, SMA, Afreen, F, Kubota, C, Kozai, T 2000Water control and survival of Ipomoea batatas grown photoautotrophically under forced ventilation and photomixotrophically under natural ventilationAnn. Bot.86603610CrossRefGoogle Scholar
  23. Zobayed, SMA, Armstrong, J, Armstrong, W 2001Leaf anatomy of in vitro tobacco and cauliflower plantlets as affected by different types of ventilationPlant Sci.161537548CrossRefGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Yoshiaki Kitaya
    • 1
    • 2
  • Yoshitaka Ohmura
    • 1
  • Chieri Kubota
    • 3
  • Toyoki Kozai
    • 1
  1. 1.Department of Bioproduction ScienceChiba UniversityMatsudoJapan
  2. 2.Graduate School of Life and Environmental SciencesOsaka Prefecture UniversityGakuen-cho, SakaiJapan
  3. 3.Department of Plant SciencesThe University of ArizonaTucsonUSA

Personalised recommendations