Abstract
Nodules of Charybdis numidica maintained in liquid Murashige and Skoog (MS) medium with 20 μ mol BA in the dark were subjected to different treatments under continuous light for shoot regeneration. A high regeneration rate without hyperhydration of the shoots was observed on semisolid basal MS medium with 1% sucrose. The use of liquid MS medium (1% sucrose, no growth regulators) resulted in a significantly lower amount of shoots per gramme of nodules under both submerged and temporary immersion (TI) conditions. Shoot hyperhydration was lowest in a TI system with one 5 min immersion every 24 h. When compared on a per container base, large amounts of shoots could be produced in the TI system with less labour input than in the system with semisolid medium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- AM:
-
semisolid medium
- BA:
-
6-benzyladenine
- LM:
-
liquid medium
- MS:
-
Murashige and Skoog medium (1962)
- TI:
-
temporary immersion
References
C Damiano A Gentile SR La Starza A Fratarelli S Monticelli (2001) Automation in plant propagation through temporary immersion systems. 1st International Symposium ‘Acclimatization and Establishment of Micropropagated Plants’ Sani-Halkidiki Greece (Abstract Book) 90
R El Grari RA Backhaus (1987) ArticleTitleIn vitro propagation of red squill, Urginea maritima Baker Plant Cell Tiss. Org. Cult. 10 65–71
BH McCown EL Zeldin HA Pinkalla R Dedolph (1988) Nodule culture: a developmental pathway with high potential for regeneration, automated micropropagation, and plant metabolite production from woody plants JW Hanover DE Keathley (Eds) Genetic Manipulation of Woody Plants Plenum Press New York 149–166
T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant. 15 473–497
SY Park HN Murthy KY Paek (2000) ArticleTitleMass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis Plant Cell Tiss. Org. Cult. 63 67–72
A Stojakowska (1993) ArticleTitleMicropropagation of Urginea maritima (L.) Baker s str. Acta Soc. Bot. Pol. 62 11–15
P Terness D Navolan Ch Dufter B Kopp G Opelz (2001) ArticleTitleThe T-cell suppressive effect of bufadienolides: structural requirements for their immunoregulatory activity Int. Immunopharmacol. 1 119–134
DL Horn ParticleVan WE Domingo (1950) ArticleTitleComparison of seed and vegetative propagation methods for red squill Econ. Bot. 4 350–353
AJ Verbiscar J Atel TF Banigan G Opelz (1986) ArticleTitleScilliroside and other Scilla compounds in red squill J. Agric. Food Chem. 34 973–979
Ch Wawrosch A Kongbangkerd B Kopp (2001) Micropropagation of Charybdis sp. (Hyacynthaceae) through nodule culture. COST843 Working Group 2 2nd Meeting Thessaloniki Greece (Abstract Book) 41–42
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wawrosch, C.h., Kongbangkerd, A., Köpf, A. et al. Shoot regeneration from nodules of Charybdis sp.: a comparison of semisolid, liquid and temporary immersion culture systems. Plant Cell Tiss Organ Cult 81, 319–322 (2005). https://doi.org/10.1007/s11240-004-6660-3
Issue Date:
DOI: https://doi.org/10.1007/s11240-004-6660-3