Advertisement

Plant Cell, Tissue and Organ Culture

, Volume 79, Issue 1, pp 63–69 | Cite as

Effects of organic solvents on membrane ofTaxus cuspidata cells in

  • Qiu-man Xu
  • Jing-sheng Cheng
  • Zhi-qiang Ge
  • Ying-jin Yuan
Article

Abstract

The effects of organic solvents (oleic acid and dibutyl phthalate) on viability and membrane integrity of Taxus cuspidata cells were investigated in two-liquid-phase suspension cultures. It has been found that the cell viability, electrical conductivity and concentration of malonyl dialdehyde did not change obviously when the content of oleic acid or dibutyl phthalate was 2% (v/v), but varied markedly when the contents of oleic acid or dibutyl phthalate were raised to 6% (v/v) or more, indicating that the organic solvents at higher concentrations severely affected the cell membrane permeability.

membrane permeability organic solvents Taxus cuspidata two-liquid-phase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Collins-Pavao M, Chin CK & Pedersen H (1996) Taxol partitioning in two-phase plant cell cultures of Taxus brevifolia. J. Biotech. 49: 95–100Google Scholar
  2. Dhindsa RS, Dhindsa PP & Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J. Exp. Bot. 32: 93–101Google Scholar
  3. Dörnenburg H & Knoor D (1996) Semicontinuous processes for anthraquinone production with immobilized Cruciata glabra cell cultures in a three-phase system. J. Biotech. 50: 55–62Google Scholar
  4. Fu XQ & Lu DL (1999) Stimulation of shikonin production by combined fungal elicitation and in situ extraction in suspension cultures of Arnebia euchroma. Enzyme Microbiol. Technol. 24: 243–246Google Scholar
  5. Gavrilov VB, Kravchenko ON & Konev SV (2000) Bases for the dye sorption as an indicator of a membrane damage and of prehemolytic conditions of the membrane. Byull. Eksp. Biol. Med. 129(3): 358Google Scholar
  6. Goodman EM, Greenebaum B & Marron MT (1995) Effects of electromagnetic fields on molecules and cells. Int. Rev. Cytol. 158: 279–338Google Scholar
  7. Harrop AJ, Woodley JM & Lilly MD (1992) Production of naphthalene-cisglycol by Pseudomonas putida in the presence of organic solvents. Enzyme Microbiol. Technol. 14: 725–730Google Scholar
  8. Heath RL & Packer L (1968) Photoperoxidation in isolated chloroplast. I. Kinetics and stochiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125: 189–198Google Scholar
  9. Iborra JL, Guardiola J, Montaner S, Canovas M & Manjon A (1992) 2,3,5-triphenyltetrazolium chloride as a viability assay for immobilized plant cells. Biotech. Tech. 6: 319–322Google Scholar
  10. Jaziri M, Zhiri A, Guo YW, Dupon JP, Shimonura K, Hamada H, Vanhaelen M & Homes J (1996) Taxus sp. cell, tissue and organ cultures as alternative sources for taxoids production: a literature survey. Plant Cell Tiss. Org. Cult. 46: 59–75Google Scholar
  11. Ketchum REB, Gibson DM, Croteau RB & Shuler ML (1999) The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotech. Bioeng. 62: 97–105Google Scholar
  12. Lanne C, Boeren S, Vos K, Veeger C (1987) Rules for optimization of biocatalysis in organic solvents, Biotech. Bioeng. 30: 81–87Google Scholar
  13. Nikolova P & Ward OP (1993) Whole cell biocatalysis in nonconventional media. J. Indust. Microbiol. 12: 76–86Google Scholar
  14. Paliyath G & Droillard MJ (1992) The mechanisms of membrane deterioration and disassembly during senescence. Plant Physiol. Biochem. 30: 789–812Google Scholar
  15. Saadalla MM, Shanahan JF & Quick JS (1990) Heat tolerance in winter wheat: I. Hardening and genetic effects on membrane thermostability. Crop Sci. 30: 1243–1247Google Scholar
  16. Sikkema J, de Bont JAM & Poolman B (1994) Interactions of cyclic hydrocarbons with biological membranes. J. Biol. Chem. 269: 8022–8028Google Scholar
  17. Sikkema J, de Bont JAM & Poolman B (1995) Mechanisms of membrane toxicity of hydrocarbons. Microbiol. Rev. 59: 201–222Google Scholar
  18. Slater TF (1984) Free-radical mechanisms in tissue injury. Biochem. J. 222: 1–15Google Scholar
  19. Suzuki K, Yano A & Shinshi H (1999) Slow and prolonged activation of the p47 protein kinase during hypersensitive cell deathinacultureoftobaccocells. Plant Physiol. 119:1465–1472Google Scholar
  20. Towill, LE & Mazur P (1974) Studies on the reduction of 2, 3, 5-triphenyltetrazolium chloride as a viability assay for plant tissue cultures. Can. J. Bot. 53: 1097–1120Google Scholar
  21. Vermue M, Sikkema J, Verheul A, Bakker R, Tramper J (1993) Toxicity of homologous series of organic solvents for the gram-positive bacteria Arthrobacter and Nocardia sp. and the gram-negative bacteria Acinetobacter and Pseudomonas sp., Biotech. Bioeng. 42: 747–758Google Scholar
  22. Wang CG, Wu JY & Mei XG (2001) Enhanced taxol production and release in Taxus chinensis cell suspension cultures with selected organic solvents and sucrose feeding. Biotech. Prog. 17: 89–94Google Scholar
  23. Wu ZL, Yuan YJ, Ma ZH & Hu ZD (2000) Kinetics of two-liquid-phase Taxus cuspidata cell culture for production of Taxol. Biochem. Eng. J. 5: 137–142Google Scholar
  24. Yuan YJ, Hu GW, Wang GG, Jing Y, Zhuo YQ & Shen PW (1998) Effect of La, Ce on Taxus cuspidata cell growth, biosynthesis and release of Taxol. J. Rare Earths 16: 300–306Google Scholar
  25. Yukimune Y, Tabata H, Higashi Y & Hara Y (1996) Methyl jasmonate-induced overproduction of paclitaxel and baccatio in Taxus cell suspension cultures. Nature Biotech. 14: 1129–1132Google Scholar
  26. Zhang SQ (1996) Handbook of Applied Fine Chemical Products (Volume Organic), Chemical Industry Press, BeijingGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Qiu-man Xu
    • 1
  • Jing-sheng Cheng
    • 1
  • Zhi-qiang Ge
    • 1
  • Ying-jin Yuan
    • 1
  1. 1.Department of Pharmaceutical EngineeringSchool of Chemical Engineering and Technology, Tianjin UniversityP.RChina

Personalised recommendations