Plant Cell, Tissue and Organ Culture

, Volume 79, Issue 1, pp 63–69 | Cite as

Effects of organic solvents on membrane ofTaxus cuspidata cells in

  • Qiu-man Xu
  • Jing-sheng Cheng
  • Zhi-qiang Ge
  • Ying-jin Yuan


The effects of organic solvents (oleic acid and dibutyl phthalate) on viability and membrane integrity of Taxus cuspidata cells were investigated in two-liquid-phase suspension cultures. It has been found that the cell viability, electrical conductivity and concentration of malonyl dialdehyde did not change obviously when the content of oleic acid or dibutyl phthalate was 2% (v/v), but varied markedly when the contents of oleic acid or dibutyl phthalate were raised to 6% (v/v) or more, indicating that the organic solvents at higher concentrations severely affected the cell membrane permeability.

membrane permeability organic solvents Taxus cuspidata two-liquid-phase 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Qiu-man Xu
    • 1
  • Jing-sheng Cheng
    • 1
  • Zhi-qiang Ge
    • 1
  • Ying-jin Yuan
    • 1
  1. 1.Department of Pharmaceutical EngineeringSchool of Chemical Engineering and Technology, Tianjin UniversityP.RChina

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