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Micropropagation of zedoary (Curcuma zedoaria Roscoe) – a valuable medicinal plant

Abstract

Tissue culture propagation system was developed for zedoary (Curcuma zedoaria Roscoe), a valuable medicinal plant, using rhizome sprout cultures. Shoots were induced from rhizomes on basal MS medium containing 20 g l−1 sucrose and 5 g l−1 agar, supplemented with 20 (v/v) coconut water (CW) and benzylaminopurine (BA) concentrations from 0.5 to 5.0 m g l−1. The excised shoots were subcultured on Murashige-Skoog (MS) medium with 20 (v/v) CW and different concentrations of BA and kinetin (Kin), either alone or in combination with indolebutyric acid (IBA) or naphthaleneacetic acid (NAA). MS medium with 20 (v/v) CW, 3 mg l−1 BA, and 0.5 mg l−1 IBA resulted in a multiplication rate per shoot; 5.6 shoots per explant were obtained on average after 30 days of culture. Well-developed shoots (30–40 mm in length) were rooted on MS medium containing 20 g l−1 sucrose and 8 g l−1 agar, supplemented with 20 (v/v) CW and 2 mg l−1 NAA. More than 95 of the rooted plants were established in pots after hardening.

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Abbreviations

AC:

activated charcoal

BA:

6-benzylaminopurine

CW:

coconut water

IBA:

indolebutyric acid

Kin:

kinetin

M ± SE:

mean ± standard error

MS:

Murashige and Skoog (1962)

NAA:

α-naphthaleneacetic acid

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Correspondence to Moon Sik Yang.

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Loc, N.H., Duc, D.T., Kwon, T.H. et al. Micropropagation of zedoary (Curcuma zedoaria Roscoe) – a valuable medicinal plant. Plant Cell Tiss Organ Cult 81, 119–122 (2005). https://doi.org/10.1007/s11240-004-3308-2

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Keywords

  • Curcuma zedoaria
  • micropropagation
  • multiple shoots
  • plantlets
  • rhizome
  • Zingiberaceae