Plant Cell, Tissue and Organ Culture

, Volume 81, Issue 1, pp 119–122 | Cite as

Micropropagation of zedoary (Curcuma zedoaria Roscoe) – a valuable medicinal plant

  • Nguyen Hoang Loc
  • Doan Trong Duc
  • Tae Ho Kwon
  • Moon Sik YangEmail author
Research note


Tissue culture propagation system was developed for zedoary (Curcuma zedoaria Roscoe), a valuable medicinal plant, using rhizome sprout cultures. Shoots were induced from rhizomes on basal MS medium containing 20 g l−1 sucrose and 5 g l−1 agar, supplemented with 20 (v/v) coconut water (CW) and benzylaminopurine (BA) concentrations from 0.5 to 5.0 m g l−1. The excised shoots were subcultured on Murashige-Skoog (MS) medium with 20 (v/v) CW and different concentrations of BA and kinetin (Kin), either alone or in combination with indolebutyric acid (IBA) or naphthaleneacetic acid (NAA). MS medium with 20 (v/v) CW, 3 mg l−1 BA, and 0.5 mg l−1 IBA resulted in a multiplication rate per shoot; 5.6 shoots per explant were obtained on average after 30 days of culture. Well-developed shoots (30–40 mm in length) were rooted on MS medium containing 20 g l−1 sucrose and 8 g l−1 agar, supplemented with 20 (v/v) CW and 2 mg l−1 NAA. More than 95 of the rooted plants were established in pots after hardening.


Curcuma zedoaria micropropagation multiple shoots plantlets rhizome Zingiberaceae 



activated charcoal




coconut water


indolebutyric acid



M ± SE

mean ± standard error


Murashige and Skoog (1962)


α-naphthaleneacetic acid


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Copyright information

© Springer 2005

Authors and Affiliations

  • Nguyen Hoang Loc
    • 1
  • Doan Trong Duc
    • 1
  • Tae Ho Kwon
    • 2
  • Moon Sik Yang
    • 3
    Email author
  1. 1.Department of Biology, College of SciencesHue UniversityHueVietnam
  2. 2.Basic Sciences Research InstituteChonbuk National UniversityJeonju
  3. 3.Division of Biological Sciences and Research Center for Bioactive MaterialsChonbuk National UniversityJeonjuRepublic of Korea

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