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Plant Cell, Tissue and Organ Culture

, Volume 81, Issue 1, pp 119–122 | Cite as

Micropropagation of zedoary (Curcuma zedoaria Roscoe) – a valuable medicinal plant

  • Nguyen Hoang Loc
  • Doan Trong Duc
  • Tae Ho Kwon
  • Moon Sik YangEmail author
Research note

Abstract

Tissue culture propagation system was developed for zedoary (Curcuma zedoaria Roscoe), a valuable medicinal plant, using rhizome sprout cultures. Shoots were induced from rhizomes on basal MS medium containing 20 g l−1 sucrose and 5 g l−1 agar, supplemented with 20 (v/v) coconut water (CW) and benzylaminopurine (BA) concentrations from 0.5 to 5.0 m g l−1. The excised shoots were subcultured on Murashige-Skoog (MS) medium with 20 (v/v) CW and different concentrations of BA and kinetin (Kin), either alone or in combination with indolebutyric acid (IBA) or naphthaleneacetic acid (NAA). MS medium with 20 (v/v) CW, 3 mg l−1 BA, and 0.5 mg l−1 IBA resulted in a multiplication rate per shoot; 5.6 shoots per explant were obtained on average after 30 days of culture. Well-developed shoots (30–40 mm in length) were rooted on MS medium containing 20 g l−1 sucrose and 8 g l−1 agar, supplemented with 20 (v/v) CW and 2 mg l−1 NAA. More than 95 of the rooted plants were established in pots after hardening.

Keywords

Curcuma zedoaria micropropagation multiple shoots plantlets rhizome Zingiberaceae 

Abbreviations

AC

activated charcoal

BA

6-benzylaminopurine

CW

coconut water

IBA

indolebutyric acid

Kin

kinetin

M ± SE

mean ± standard error

MS

Murashige and Skoog (1962)

NAA

α-naphthaleneacetic acid

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References

  1. Balachandran, SM, BhatSR& Chandel, KPS 1990In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.)Plant Cell Rep.8521524Google Scholar
  2. Borthakur, M, Bordoloi, DN 1992Micropropagation of Curcuma amada RoxbJ. Spice Aromat. Crops1154156Google Scholar
  3. Matsuda, H, Ninomiya, T, Yoshikawa, M 1998Inhibitory effect and action mechanism of sesquiterpenes from Zedoariae rhizoma on D-galactosamine/lipopolysaccharide-induced liver injuryBioorg. Med. Cherm. Lett.8339344Google Scholar
  4. Mukhri, Z, Yamaguchi, H 1986In vitro plant multiplication from rhizomes of turmeric (Curcuma domestica Val.) and Temoe Lawak (C. xanthorhiza Roxb.)Plant Tiss Cult. Lett.12830Google Scholar
  5. Murashige, T, Skoog, F 1962A revised medium for rapid growth and bioassays with tobacco tissue culturePhysiol. Plant15473497Google Scholar
  6. Nadgauda, RS, Mascarenhas, AF, Hendre, RR, Jagannathan, V 1978Rapid multiplication of turmeric (Curcuma longa L)India J. Exp. Biol.16120122Google Scholar
  7. Nayak, S 2000In vitro multiplication and microrhizome induction in Curcuma aromatica SalisbPlant Growth Regul.324147Google Scholar
  8. Prathanturarug, S, Soonthornchareonnon, N, Chuakul, W, Phaidee, Y, Sasalamp, P 2003High-frequency shoot multiplication in Curcuma longa L using thidiazuronPlant Cell Rep.2110641059Google Scholar
  9. Salvi, ND, George, L, Eapen, S 2002Micropropagation and field evaluation of micropropagated plants of turmericPlant Cell Tiss. Org. Cult.68143151Google Scholar
  10. Syu, W,Jr, Shen, CC, Don, MJ, Ou, JC, Lee, GH, Sun, CM 1998Cytotoxicity of curcuminoids and some novel compounds from Curcuma zedoariaJ. Nat. Prod.6115311534Google Scholar
  11. Wannakrairoj, S 1997Clonal micropropagation of patumma (Curcuma alismatifolia Gagnep.). Kas JNat. Sci.31353356Google Scholar
  12. Yoshioka, T, Fujii, E, Endo, M, Wada, K, Tokunaga, Y, Shiba, N, Hohsho, H, Shibuya, H, Muraki, T 1998Antiinflammatory potency of dehydrocurdione, a zedoary-derived sesquiterpeneInflamm. Res.47476481Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Nguyen Hoang Loc
    • 1
  • Doan Trong Duc
    • 1
  • Tae Ho Kwon
    • 2
  • Moon Sik Yang
    • 3
    Email author
  1. 1.Department of Biology, College of SciencesHue UniversityHueVietnam
  2. 2.Basic Sciences Research InstituteChonbuk National UniversityJeonju
  3. 3.Division of Biological Sciences and Research Center for Bioactive MaterialsChonbuk National UniversityJeonjuRepublic of Korea

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