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Study of embryo rescue in floribunda rose

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Abstract

In the past few decades, breeders have faced a lot of problems in rose improvement due to low sexual reproduction and poor germination because of embryo abortion. Immature embryos may be recovered in vitro. An efficient protocol for embryo rescue in two floribunda roses (‘Arunima’ and ‘Shocking Blue’) was developed. The germination of immature embryos was achieved by manipulating the growth media, growth hormones and culture conditions. The embryos (rescued) germinated and grew considerably on Murashige and Skoog basal medium supplemented with 2.5 mg l−l BA (6-benzylaminopurine), 0.5 mg l−l GA3 (gibberellic acid) and 3 (w/v) sucrose under 16-h photoperiod. A higher rate of germination was observed in cultures incubated 2 weeks in dark and subsequently transferred to 2 weeks in light at 16-h photoperiod. The embryo derived plantlets were successfully transferred to greenhouse and produced flowers. Embryo rescue technique in floribunda roses has great potential in floriculture industry.

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Abbreviations

BA:

6-benzylaminopurine

2,4-d:

2,4-dichlorophenoxy acetic acid

GA3:

gibberellic acid

IAA:

indole-3-acetic acid

Kn:

kinetin

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Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Plant Biotechnology Division, Regional Plant Resource Centre, Bhubaneswar, 751 015, Orissa, India

    Anuradha Mohapatra & G. R. Rout

Authors
  1. Anuradha Mohapatra
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  2. G. R. Rout
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Correspondence to G. R. Rout.

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Mohapatra, A., Rout, G.R. Study of embryo rescue in floribunda rose. Plant Cell Tiss Organ Cult 81, 113–117 (2005). https://doi.org/10.1007/s11240-004-3128-4

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  • DOI: https://doi.org/10.1007/s11240-004-3128-4

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