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Regeneration and Transformation of Echinops cv Veitsch Blue

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Abstract

Echinops Asteraceae/Compositae is widespread throughout southern Europe and Asia. It is a native plant in Israel and usually found as a weed in wheat fields. Due to the need of the plants for vernalization, horticultural practice is to chill the crowns for 2 month before planting to achieve early flowering. A method for the propagation of Echinops through axillary buds and root cutting cultures is described in this manuscript. Best conditions for propagation were using MS salt medium supplemented with 3 sucrose and 1 mg l-1 IAA plus 0.5 mg l-11 BA under 16 h of cool fluorescent light. Root cuttings produced shoots all along the root but each growth regulator combination affected where roots will sprout shoots. Rooted plants were successfully acclimatized after propagating on the above medium and eventually flowered. Agrobacterium transformation of Echinops was successfully achieved and expression of GUS reporter gene was tested under the control of two constitutive promoters.

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Abbreviations

BA:

6-Benzyladenine

CTAB:

Hexadecyltrimethylammonium bromide

EDTA:

Na2-Ethylenediaminetetraacitic dihydrate

IAA:

Indole-3-acetic acid

IBA:

3-Indole butyric acid

MS:

Murashige and Skoog salt medium

NAA:

1-Naphtalene acetic acid

TDZ:

Thidiazuron

WPM:

Woody Plant Medium

β-ME:

β-Mercaptoethanol

References

  • Duzenli A and Cakan H (2001). Flora of Mount Musa (Hatay-Turkey). Turk. J. Bot. 25: 285–309

    Google Scholar 

  • Famiani F, Ferradini N, Staffolani P and Standardi A (1994). Effect of leaf excision time and age, BA concentration and dark treatments on in vitro shoot regeneration of M.26 apple rootstock. J. Hortic. Sci. 69: 679–685

    Google Scholar 

  • Garbarino JE, Oosumi T and Belknap WR (1995). Isolation of a polyubiquitin promoter and its expression in transgenic potato plants. Plant physiol 109: 1371–1378

    Article  PubMed  CAS  Google Scholar 

  • Hahn P and Naser K (1979). Cultivation of herbaceous perennials for production of cut flowers in the open-species worth recommending for cut flower supply in the spring and summer months. Gartenbau Wissenschaf 26: 341–344

    Google Scholar 

  • Iversen RR and Weiler TC (1994). Strategies to force flowering of six herbaceous garden perennials. Hort Technol. 4: 61–65

    Google Scholar 

  • Jefferson RA (1987). Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5: 387–405

    CAS  Google Scholar 

  • Ke J, Kahn R, Johnson T, Somers DA and Das A (2001). High-efficiency gene transfer to recalcitrant plants by Agrobacrerium tumefaciens Plant Cell Rep. 20: 150–156

    Article  CAS  Google Scholar 

  • Lin HS, Jeu MJ and Jacobsen E (1998). Fromation of shoots from leaf axils of Alstroemeria: the effect of the position on the stem. Plant Cell, Tiss.-Org.-Cult. 52: 165–169

    Article  CAS  Google Scholar 

  • Ma B, Tatumoto I, Ogawa T, Morikawa T, Ma B (2002) Micro-propagation using leaf blades in genus Lycoris. Scientific report of the graduate school of agriculture and biological sciences, Osaka, Japan. 54: 1–5

  • McCown BH and Llyod C (1981). Woody Plant Medium (WPM): a revised mineral nutrient formulation for microculture of woody plant species. Hort. Sci. 16: 453

    Google Scholar 

  • Murch SJ, Wierenga EJ, El-Demerdash MA and Saxena PK (2003). In vitro propagation of the Egyptian medicinal plant, Echinops spinosissimus Turra. Plant Cell Tis. Org. Cul. 74(1): 81–86

    Article  CAS  Google Scholar 

  • Pan ZG, Liu CZ., MUrch SJ, EL-Demerdash M and Saxena PK (2003). Plant regeneration from mesophyll protoplasts of the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra. Plant Sci. 165(4): 681–687

    Article  CAS  Google Scholar 

  • Pedroso MC and Pais MS (1993). Direct embryo formation in leaves of Camellia japonica L. Plant Cell Rep. 12: 639–643

    Google Scholar 

  • Rimbau V, Cerdan C, Vila R and Iglesias J (1999). Antiinflammatory activity of some extracts from plants used in the traditional medicine of North-African countries (II). Phytother. Res. 13: 128–132

    Article  PubMed  CAS  Google Scholar 

  • Sathiyamoorthy P, Lugasi-Evgi H, Schlesinger P, Kedar I, Gopas J, Pollack Y and Golan-Goldhirsh A (1999). Screening for cytotoxic and antimalarial activities in desert plants of the Negev and bedouin market plant products. Pharm. Biol. 37: 188–195

    Article  Google Scholar 

  • Shah MJ, Subhan F, Tahir F and Alam W (1997). Male contraceptives from traditional drugs (plant based). Hamdard Medicus. 40: 34–36

    Google Scholar 

  • Sheidai M, Nasirzadeh A and Kheradnam M (2000). Karyotypic study of Echinops (Asteraceae) in Fars province. Iran. Bot. J. Lin. Soc. 134: 453–463

    Article  Google Scholar 

  • Shibli RA, Ajlouni MMA and Obeidat AA (2000). Direct regeneration from wild pear (Pyrus syniaca) leaf explants. Adv. Hortic. Sci. 14: 12–18

    Google Scholar 

  • Surridge C (2004). Plant development: the flowers that bloom in the spring. Nature 427: 112

    Article  PubMed  CAS  Google Scholar 

  • Tel-zur N, Abbo S, Myslabodski D and Mizrahi Y (1999). Modified CTAB procedure for DNA isolation from epiphytic cacti of the genera Hylocereus and Selenicereus (Cactaceae). Plant Mol. Biol. Report. 17: 249–254

    Article  CAS  Google Scholar 

  • Wallerstein I (1983). The flowering of Echinops blancheanus Boiss. Hassadeh. (in Hebrew) 63: 992–993

    Google Scholar 

  • Weiss D. and Ziv O (1999). Agrothechnical and physiological factors that are involved in the control of flowering in Echinops Dapi Maida (in Hebrew) 15: 63–64

    Google Scholar 

  • Zhang Y and Zhang YH (1999). Effect of different explants and phyto-hormones on organogenesis in scarlet eggplant (Solanum aethiopicum L). J. Yunnan Agric. U. 14: 279–283

    Google Scholar 

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Authors and Affiliations

  1. Department of Ornamental Horticulture, ARO Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel

    Evenor Dalia & Reuveni Moshe

  2. Institutes for Applied Research, Ben Gurion University of the Negev, P.O. Box 356, Beer Sheva, 84105, Israel

    Zaccai Michele

  3. Department of Landscape Architecture, Beijing Agricultural College, Beijing, 102206, China

    Chen Xinlu

Authors
  1. Evenor Dalia
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  2. Zaccai Michele
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  3. Chen Xinlu
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  4. Reuveni Moshe
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Correspondence to Reuveni Moshe.

Additional information

This is publication number 05/03 from Horticulture Institute ARO.

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Dalia, E., Michele, Z., Xinlu, C. et al. Regeneration and Transformation of Echinops cv Veitsch Blue. Plant Cell Tiss Organ Cult 85, 1–9 (2006). https://doi.org/10.1007/s11240-004-2564-5

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  • DOI: https://doi.org/10.1007/s11240-004-2564-5

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