Abstract
Suspension cultures from Arabidopsis thaliana wild type and AtPIN1-deficient lines were initiated and maintained for more than 3 years. A protocol for efficient regeneration from long-term suspension cultures was established. Arabidopsis wild-type and respectively AtPIN1 mutant plants have been regenerated from these cultures and characterized. Additionally, transgenic suspension cultures expressing the uidA (β -glucuronidase) reporter gene under the control of AtPIN1 promoter have been used for morphogenic studies. Our studies suggest that a lack of AtPIN1 function affects shoot differentiation and development, but does not influence in vitro regeneration of plants.
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Eneva, T., Tietz, O., Luley, E. et al. Loss of AtPIN1 does not influence the in vitro morphogenic potential of Arabidopsis thaliana suspension cultures. Plant Cell, Tissue and Organ Culture 79, 181–188 (2004). https://doi.org/10.1007/s11240-004-0658-8
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DOI: https://doi.org/10.1007/s11240-004-0658-8