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Expression of a cucumber class III chitinase and Nicotiana plumbaginifoliaclass I glucanase genes in transgenic potato plants

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Abstract

The genes encoding for a cucumber class III chitinase and Nicotiana plumbaginifolia class I glucanase were co-introduced into Slovak potato (Solanum tuberosum L.) breeding line 116/86 using Agrobacterium tumefaciens. For both transgenes the number of integrated copies and level of RNA expression were determined. These analyses demonstrated low variation and significant correlation in expression of the introduced transgenes. The effect of transgene expression on fungal susceptibility of transformants was evaluated in vitro. Hyphal extension assays revealed no obvious differences in the ability of extracts from transformants to inhibit growth of Rhizoctonia solani comparing to non-transformed potato.

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Authors and Affiliations

  1. Institute of Plant Genetics and Biotechnology SAS, Akademická 2, P.O. Box 39A, 950, 07, Nitra, Slovak Republic

    Jana Moravčíková, Ildikó Matušíková, Jana Libantová & L'udmila Mlynárová

  2. Research Institute of Animal Production, Hlohovská 2, 949 92, Nitra, Slovak Republic

    Miroslav Bauer

  3. Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands

    L'udmila Mlynárová

  4. Institute of Vascular Biology and Thrombosis Research of Vienna, Schwarzspanierstr., 17, A-1090, Vienna, Austria

    Miroslav Bauer

Authors
  1. Jana Moravčíková
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  2. Ildikó Matušíková
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  3. Jana Libantová
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  4. Miroslav Bauer
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  5. L'udmila Mlynárová
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Correspondence to Jana Moravčíková.

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Moravčíková, J., Matušíková, I., Libantová, J. et al. Expression of a cucumber class III chitinase and Nicotiana plumbaginifoliaclass I glucanase genes in transgenic potato plants. Plant Cell, Tissue and Organ Culture 79, 161–168 (2004). https://doi.org/10.1007/s11240-004-0656-x

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  • DOI: https://doi.org/10.1007/s11240-004-0656-x

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