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Transient expression of heterologous model gene in plants using Potato virus X-based vector

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Abstract

To optimize the efficiency of expression of foreign proteins using Potato virus X (PVX) -- based vector, the gene for the coat protein (CP) of other virus (Potato virus A, PVA) was cloned into the vector, propagated in E. coli and subsequently inoculated or agroinfected into the host plants. Host range studies showed that the best host plant is N. benthamiana. By means of RT PCR the presence and the stability of the construct were tested. Both ELISA and Western blot analysis were applicable for expressed protein detection. Expression level of PVA CP achieved approximately 5--10 per mille of total soluble proteins. The results demonstrated that agroinfection is the most suitable method for the propagation of our model gene using PVX--based vectors.

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Authors and Affiliations

  1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1a, Czech Republic

    Noemi Čeřovská, Tamara Pečenková, Tomáš Moravec & Jiří Velemínský

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  1. Noemi Čeřovská
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  2. Tamara Pečenková
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  3. Tomáš Moravec
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  4. Jiří Velemínský
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Čeřovská, N., Pečenková, T., Moravec, T. et al. Transient expression of heterologous model gene in plants using Potato virus X-based vector. Plant Cell, Tissue and Organ Culture 79, 147–152 (2004). https://doi.org/10.1007/s11240-004-0654-z

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  • DOI: https://doi.org/10.1007/s11240-004-0654-z

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