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Profibrinolytic effect of Enzamin, an extract of metabolic products from Bacillus subtilis AK and Lactobacillus

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Abstract

Fibrinolytic system impairment contributes to the development of thrombotic disease such as cardiovascular disease and stroke. Therefore, an agent that increases fibrinolytic activity may be useful for the prevention of these diseases. In this study, to explore novel profibrinolytic agents, we examined the profibrinolytic effect of Enzamin, an extract of metabolic products from Bacillus subtilis AK and Lactobacillus in vitro and in vivo. Enzamin directly enhanced plasmin activity generated by tissue-type plasminogen activator (t-PA) by twofold but not by urokinase-type plasminogen activator (u-PA) in vitro, which was measured employing both the chromogenic substrate H-d-Val-Leu-Lys-pNA (S-2251) and fibrin plate. Enzamin also increased plasmin activity generated by t-PA in the cell lysate and culture medium of endothelial cells, measured by fibrin zymography. Furthermore, the oral administration of a 1% concentration of Enzamin increased plasmin activity generated by t-PA by 1.7-fold but not by u-PA in the euglobulin fraction of mouse plasma. In conclusion, Enzamin has a unique ability to enhance the fibrinolytic activity through an increase in endogenous plasmin activity generated by t-PA released from endothelial cells, and may be a beneficial supplement for the prevention of thrombotic episodes.

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Acknowledgments

We thank Mihoko Iwata (Kinki University) for excellent technical assistance.

Conflict of interest

The authors state that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Physiology, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osakasayama, Osaka, 589-8511, Japan

    Yukinori Tamura, Kiyotaka Okada, Naoyuki Kawao, Masato Yano, Shigeru Ueshima, Nobuo Nagai & Osamu Matsuo

  2. Department of Food Science and Nutrition, Kinki University School of Agriculture, 3327-204, Nakamachi, Nara, 631-8505, Japan

    Shigeru Ueshima

  3. Department of Animal Bio-Science, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-cho, Nagahama, Shiga, 526-0829, Japan

    Nobuo Nagai

Authors
  1. Yukinori Tamura
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  2. Kiyotaka Okada
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  3. Naoyuki Kawao
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  4. Masato Yano
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  5. Shigeru Ueshima
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  6. Nobuo Nagai
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  7. Osamu Matsuo
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Correspondence to Osamu Matsuo.

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Tamura, Y., Okada, K., Kawao, N. et al. Profibrinolytic effect of Enzamin, an extract of metabolic products from Bacillus subtilis AK and Lactobacillus . J Thromb Thrombolysis 32, 195–200 (2011). https://doi.org/10.1007/s11239-011-0552-2

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  • DOI: https://doi.org/10.1007/s11239-011-0552-2

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