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Different clinical models of CD34 + cells mobilization in patients with cardiovascular disease

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Abstract

To test the role of necrosis, ischemia or both in bone marrow cells (BMC) mobilization in patients with cardiovascular disease. We studied three groups of patients: group 1, Iatrogenic Necrosis, with pure necrosis (28 patients undergoing transcatheter radiofrequency ablation); group 2, Ischemic Necrosis (30 patients with myocardial infarction); group 3, Pure Ischemia (24 patients with unstable angina). As control groups, we studied 27 patients with stable coronary artery disease (CAD), and 20 patients without CAD undergoing angiography for valvular diseases or cardiomiopathy. CD34 + cells and cytokines were evaluated at: T0 (baseline), 48 h and 5, 7, 10, 14 days thereafter. We observed a significant increase of CD34 + cells at T3 and T4 only in Iatrogenic Necrosis and Ischemic Necrosis group. The peak of mobilization was observed ten days after the necrotic event (2.8 ± 1.4 vs. 5.9 ± 1.9 in the group 1, P = 0.03; and 3 ± 1.5 vs. 5.6 ± 2 in the group 2, P = 0.04; respectively). We found a good correlation between CD34 + and vascular endothelial growth factor (VEGF) and stromal derived factor (SDF-1α) peak values (r = 0.77 and r = 0.63, respectively). At multivariable analysis, myocardial necrosis (OR 3.5, 95%CI 2.2–4.2, P < 0.01), VEGF (OR 2, 95%CI 1.1–3, P = 0.01 as above versus below median value), and SDF-1α (OR 1.6, 95%CI 1.1–2.5, P = 0.02 as above versus below median value) emerged as independent predictors of C34 + cells increase. Myocardial necrosis with simultaneous elevation of VEGF and SDF-1α causes a significant CD34 + cells mobilization in patients with cardiovascular disease.

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Acknowledgments

The authors thank Dr Monia Monti, Dr Stefania Gambetti and Dr Laura Bristot (Medical Trials Analysis, Ferrara, Italy) for their assistance in the data collecting and laboratory tests. The study was supported by a Regional-University project of Emilia Romagna–Area IB–Innovative Research grant.

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Authors and Affiliations

  1. U.O. Cardiologia, Cardiovascular Institute, Azienda Ospedaliero-Universitaria S.Anna, Corso Giovecca, 203, 44100, Ferrara, Italy

    Elisa Cangiano, Caterina Cavazza, Gianluca Campo, Marco Valgimigli, Patrizia Malagutti, Claudio Pratola & Roberto Ferrari

  2. Cardiovascular Research Centre, Salvatore Maugeri Foundation, IRCCS Lumezzane e Ferrara, Ferrara, Italy

    Marco Valgimigli, Gloria Francolini & Roberto Ferrari

Authors
  1. Elisa Cangiano
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  2. Caterina Cavazza
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  3. Gianluca Campo
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  4. Marco Valgimigli
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  5. Gloria Francolini
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  6. Patrizia Malagutti
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  7. Claudio Pratola
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  8. Roberto Ferrari
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Correspondence to Elisa Cangiano.

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Cangiano, E., Cavazza, C., Campo, G. et al. Different clinical models of CD34 + cells mobilization in patients with cardiovascular disease. J Thromb Thrombolysis 32, 1–8 (2011). https://doi.org/10.1007/s11239-010-0543-8

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  • DOI: https://doi.org/10.1007/s11239-010-0543-8

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