Skip to main content
SpringerLink
Log in

The effects of coronary artery disease severity on time-dependent changes in platelet activation indices in stored whole blood

  • Published:
Journal of Thrombosis and Thrombolysis Aims and scope Submit manuscript

Abstract

Background

Platelets play a pivotal role in the pathogenesis of the thrombotic complications in cardiovascular disease (CVD). Abnormal platelet activation indices are evolving as potentially useful markers in CVD risk stratification. Whilst there has been some investigation into the effects of storage time on several of these indices, the effects of underlying disease severity on these temporal changes have not been previously studied.

Methods

Using the ADVIATM120 haematology analyser, we assessed the effects of time-dependent storage of whole blood in EDTA, on a number of platelet activation indices: mean platelet volume (MPV), mean platelet component (MPC, measure of platelet density) and platelet component distribution width (PCDW, a marker of platelet shape change. We studied three age- and sex-matched patient groups: (i) healthy controls (n = 10), (ii) stable patients with coronary artery disease (CAD, n = 9); and (iii) patients with acute myocardial infarction (n = 8). Whole blood samples were processed at exactly 5 min following venesection and at 15, 30, 60 and 120 min later in storage in EDTA tubes at room temperature.

Results

There was a significant and stepwise increase in MPV (P = 0.01) and decrease in PCDW (P = 0.03), with a non-significant trend to increasing MPM and decreasing MPC with increasing underlying disease (that is healthy, ‘stable’ and ‘acute’ artery disease). There was a significant time-dependent increase in MPV and decrease in MPC and PCDW (all P < 0.05), which were all significant on ‘post-hoc’ analyses by 30 min. There were no significant changes in platelet count or MPM with time. There was no interaction of underlying disease with whole-blood storage time for any of the platelet indices reported (P = NS).

Conclusion

There is a temporal increase in MPV and decrease in MPC and PCDW in venous blood stored over 2 h in EDTA. These changes are not influenced by the underlying CVD disease severity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Boos CJ, Lip GY (2006) Platelet activation and cardiovascular outcomes in acute coronary syndromes. J Thromb Haemost 4(12):2542–2543

    Article  PubMed  CAS  Google Scholar 

  2. Tsiara S, Elisaf M, Jagroop IA, Mikhailidis DP (2003) Platelets as predictors of vascular risk: is there a practical index of platelet activity? Clin Appl Thromb Hemost 9:177–190

    Article  PubMed  CAS  Google Scholar 

  3. Jesty J, Yin W, Perrotta P, Bluestein D (2003) Platelet activation in a circulating flow loop: combined effects of shear stress and exposure time. Platelets 14:143–149

    Article  PubMed  CAS  Google Scholar 

  4. Boos CJ, Lip GY (2006) Assessment of mean platelet volume in coronary artery disease – what does it mean? Thromb Res 120(1):11–13

    Article  PubMed  CAS  Google Scholar 

  5. Ruf A, Patscheke H (1995) Flow cytometric detection of activated platelets: comparison of determining shape change, fibrinogen binding, and P-selectin expression. Semin Thromb Hemost 21:146–151

    PubMed  CAS  Google Scholar 

  6. Gurney D, Lip GY, Blann AD (2002) A reliable plasma marker of platelet activation: does it exist? Am J Hematol 70:139–144

    Article  PubMed  CAS  Google Scholar 

  7. Kamath S, Blann AD, Lip GY (2001) Platelet activation: assessment and quantification. Eur Heart J 22:1561–1571

    Article  PubMed  CAS  Google Scholar 

  8. Ahnadi CE, Sabrinah Chapman E, Lepine M, Okrongly D, Pujol-Moix N, Hernandez A, Boughrassa F, Grant AM (2003) Assessment of platelet activation in several different anticoagulants by the Advia 120 Hematology System, fluorescence flow cytometry, and electron microscopy. Thromb Haemost 90:940–948

    PubMed  CAS  Google Scholar 

  9. Boos CJ, Beevers DG, Lip GYH (2007) Assessment of platelet activation indices using the ADVIATM 120 amongst ‘high-risk’ patients with hypertension. Ann Med 39:72–78

    Article  PubMed  Google Scholar 

  10. Giacomini A, Legovini P, Antico F, Valverde S, Salvadego MM, Manoni F, Gessoni G (2003) Assessment of in vitro platelet activation by Advia 120 platelet parameters. Lab Hematol 9:132–137

    PubMed  CAS  Google Scholar 

  11. Papanas N, Symeonidis G, Maltezos E, Mavridis G, Karavageli E, Vosnakidis T, Lakasas G (2004) Mean platelet volume in patients with type 2 diabetes mellitus. Platelets 15:475–478

    Article  PubMed  CAS  Google Scholar 

  12. Greisenegger S, Endler G, Hsieh K, Tentschert S, Mannhalter C, Lalouschek W (2004) Is elevated mean platelet volume associated with a worse outcome in patients with acute ischemic cerebrovascular events? Stroke 35:1688–1691

    Article  PubMed  CAS  Google Scholar 

  13. Martin JF, Bath PM, Burr ML (1991) Influence of platelet size on outcome after myocardial infarction. Lancet 338:1409–1411

    Article  PubMed  CAS  Google Scholar 

  14. Bertrand ME, Simoons ML, Fox KA, Wallentin LC, Hamm CW, McFadden E, De Feyter PJ, Specchia G, Ruzyllo W (2002) Task Force on the Management of Acute Coronary Syndromes of the European Society of Cardiology. Management of acute coronary syndromes in patients presenting without persistent ST-segment elevation. Eur Heart J 23:1809–1840

    Article  PubMed  Google Scholar 

  15. Van de Werf F, Ardissino D, Betriu A, Cokkinos DV, Falk E, Fox KA, Julian D, Lengyel M, Neumann FJ, Ruzyllo W, Thygesen C, Underwood SR, Vahanian A, Verheugt FW, Wijns W (2003) Task Force on the Management of Acute Myocardial Infarction of the European Society of Cardiology. Management of acute myocardial infarction in patients presenting with ST-segment elevation. Eur Heart J 24:28–66

    Article  PubMed  Google Scholar 

  16. Macey M, Azam U, McCarthy D, Webb L, Chapman ES, Okrongly D, Zelmanovic D, Newland A (2002) Evaluation of the anticoagulants EDTA and citrate, theophylline, adenosine, and dipyridamole (CTAD) for assessing platelet activation on the ADVIA 120 hematology system. Clin Chem 48:891–899

    PubMed  CAS  Google Scholar 

  17. Lim YA, Hyun BH (2002) Evaluation of platelet parameters on the ADVIA 120 as the quality indicator for stored platelets. Clin Lab Haematol 24:377–384

    Article  PubMed  CAS  Google Scholar 

  18. Lippi G, Salvagno GL, Solero GP, Franchini M, Guidi GC (2005) Stability of blood cell counts, hematologic parameters and reticulocytes indexes on the Advia A120 hematologic analyzer. J Lab Clin Med 146:333–340

    Article  PubMed  CAS  Google Scholar 

  19. Song KS, Song JW (2005) Changes of platelet parameters determined by the Bayer ADVIA 120 with EDTA sample age. Platelets 16:223–224

    Article  PubMed  CAS  Google Scholar 

  20. Mathur A, Robinson MS, Cotton J, Martin JF, Erusalimsky JD (2001) Platelet reactivity in acute coronary syndromes: evidence for differences in platelet behaviour between unstable angina and myocardial infarction. Thromb Haemost 85:989–994

    PubMed  CAS  Google Scholar 

  21. Kilicli-Camur N, Demirtunc R, Konuralp C, Eskiser A, Basaran Y (2005) Could mean platelet volume be a predictive marker for acute myocardial infarction? Med Sci Monit 11:387–392

    Google Scholar 

  22. Khandekar MM, Khurana AS, Deshmukh SD, Kakrani AL, Katdare AD, Inamdar AK (2006) Platelet volume indices in patients with coronary artery disease and acute myocardial infarction: an Indian scenario. J Clin Pathol 59:146–149

    Article  PubMed  CAS  Google Scholar 

  23. Thompson CB, Love DG, Quinn PG, Valeri CR (1983) Platelet size does not correlate with platelet age. Blood 62:487–494

    PubMed  CAS  Google Scholar 

  24. Jackson SR, Carter JM (1993) Platelet volume: laboratory measurement and clinical application. Blood Rev 7:104–113

    Article  PubMed  CAS  Google Scholar 

  25. Senaran H, Ileri M, Altinbas A, Kosar A, Yetkin E, Ozturk M, Karaaslan Y, Kirazli S (2001) Thrombopoietin and mean platelet volume in coronary artery disease. Clin Cardiol 24:405–408

    Article  PubMed  CAS  Google Scholar 

  26. Van Cott EM, Fletcher SR, Kratz A (2005) Effects of the blood-collection tube material and long-term storage on platelet activation parameters on the ADVIA 120/2120 hematology system. Lab Hematol 11:71–75

    Article  PubMed  Google Scholar 

  27. Macey MG, Carty E, Webb L, Chapman ES, Zelmanovic D, Okrongly D, Rampton DS, Newland AC (1999) Use of mean platelet component to measure platelet activation on the ADVIA 120 haematology system. Cytometry 38:250–255

    Article  PubMed  CAS  Google Scholar 

  28. Macey M, McCarthy D, Azam U, Milne T, Golledge P, Newland A (2003) Ethylenediaminetetraacetic acid plus citrate-theophylline-adenosine-dipyridamole (EDTA-CTAD): a novel anticoagulant for the flow cytometric assessment of platelet and neutrophil activation ex vivo in whole blood. Cytometry B Clin Cytom 51:30–40

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

We acknowledge the financial support of the Sandwell and West Birmingham Hospitals NHS Trust Research and Development programme for the Haemostasis, Thrombosis and Vascular Biology Unit.

Author information

Authors and Affiliations

  1. Haemostasis, Thrombosis and Vascular Biology Unit, University Department of Medicine, City Hospital, Birmingham, B18 7QH, UK

    Christopher J. Boos, Balu Balakrishnan & Gregory Y. H. Lip

Authors
  1. Christopher J. Boos
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Balu Balakrishnan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gregory Y. H. Lip
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gregory Y. H. Lip.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Boos, C.J., Balakrishnan, B. & Lip, G.Y.H. The effects of coronary artery disease severity on time-dependent changes in platelet activation indices in stored whole blood. J Thromb Thrombolysis 25, 135–140 (2008). https://doi.org/10.1007/s11239-007-0034-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11239-007-0034-8

Keywords

Search

Navigation