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Morphological and molecular description of Dictyocaulus xanthopygus sp. nov. (Nematoda: Trichostrongyloidea) from the Manchurian wapiti Cervus elaphus xanthopygus

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Abstract

Dictyocaulus xanthopygus sp. nov. (Nematoda: Trichostrongyloidea) was isolated from the lungs of the Manchurian wapiti in Primorsky kray, Russia. The newly described species exhibits morphological characteristics of Dictyocaulus but is distinct from congeneric species based on morphological (lengths of body and esophagus, distances from the anterior end to nerve ring and to excretory pore, the thickness of the buccal capsule, etc.) and molecular features. High levels of genetic divergence as well as Bayesian phylogenetic analyses based on 18S rRNA nuclear and cox1 mitochondrial genes supported the independence of Dictyocaulus xanthopygus sp. nov. Secondary structures of helix 39 of 18S rRNA were identical, while ES9 adjacent to the helix has a unique conformation for newly described worms. Energy-efficient conformational rearrangements of rRNA secondary structures can be applicable in studies on the pathogenesis, epidemiology, taxonomy and evolutionary biology of parasites. Additionally, bracketed dichotomous keys to six valid species of Dictyocaulus were prepared.

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Authors and Affiliations

  1. Pacific Branch of the Federal State Budget Scientific Institution “Russian Federal Research Institute of Fisheries and oceanography”, 4 Alley Shevchenko, Vladivostok, Russian Federation, 690091

    Konstantin S. Vainutis, Anastasia N. Voronova & Mark E. Andreev

  2. The Far Eastern State Technical Fisheries University (FESTFU), 52B Lugovaya Street, Vladivostok, Russian Federation, 690087

    Konstantin S. Vainutis

  3. Far Eastern Federal University, Vladivostok, Russia, 690091

    Mark E. Andreev & Mikhail Yu. Shchelkanov

  4. G.P. Somov Research Institute of Epidemiology and Microbiology, Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Vladivostok, Russia, 690087

    Dmitry V. Pankratov & Mikhail Yu. Shchelkanov

  5. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the RAS, Vladivostok, Russia, 690022

    Mikhail Yu. Shchelkanov

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  1. Konstantin S. Vainutis
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Contributions

KV and AV designed the research; KV, AV and MA substantially participated in improvement of research and manuscript writing; KV and MA isolated material, performed the zoological and phylogenetic analysis; MA prepared Figs.; DP and MSh presented the material; MSh provide departmental control, financial support and materials for studies. All authors contributed to the article and approved the submitted version.

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Correspondence to Konstantin S. Vainutis.

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Vainutis, K.S., Voronova, A.N., Andreev, M.E. et al. Morphological and molecular description of Dictyocaulus xanthopygus sp. nov. (Nematoda: Trichostrongyloidea) from the Manchurian wapiti Cervus elaphus xanthopygus. Syst Parasitol 100, 557–570 (2023). https://doi.org/10.1007/s11230-023-10105-4

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