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Isospora leptopogoni n. sp. (Apicomplexa: Eimeriidae) from the sepia-capped flycatcher Leptopogon amaurocephalus Tschudi, 1846 (Passeriformes: Rhynchocyclidae) in South America

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Abstract

Coccidian protozoan species recorded from flycatchers are few, but they have been described with a certain frequency in recent years. In this context, the present study describes a new Isospora sp. from sepia-capped flycatchers Leptopogon amaurocephalus Tschudi, 1846 captured in the Itatiaia National Park and in a reforestation area which is about 60 km away from the park boundaries, in addition to providing a molecular identification via sequencing of the mitochondrial cytochrome c oxidase subunit 1 gene. Isospora leptopogoni n. sp. has oöcysts that are subspheroidal to ovoidal, measuring on average 22.0 × 19.7 μm, with a smooth, bi-layered wall, c.1.7 μm thick. The micropyle is delicate or inconspicuous. Oöcyst residuum is absent, but one to three polar granules are present. Sporocysts are lemon-shaped, measuring on average 14.7 × 9.3 μm, with a knob-like Stieda body and a rectangular to rounded sub-Stieda body. Sporocyst residuum is present, consisting of compactly bounded granules. Sporozoites are vermiform, with refractile bodies and nucleus. Isospora leptopogoni is different from other Isospora spp. mainly due to its lemon-shaped sporocysts, the presence of micropyle and details of Stieda and sub-Stieda bodies. Phylogenetic analysis placed I. leptopogoni close to other Isospora spp. recorded from phylogenetically related hosts and from the same biogeographic region. Finally, the recurrent finding of this coccidian species in the same L. amaurocephalus specimen in a specific locality in the Itatiaia National Park suggests that the dispersion of I. leptopogoni needs continuous transmissions between susceptible passerines as the area of movement of each L. amaurocephalus specimen appears to be quite small.

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Acknowledgements

We are thankful to staff at the Parque Nacional do Itatiaia, mainly to the research coordinators Dr Léo Nascimento (previous) and Marcelo Souza Motta (current); and the Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Campus Pinheiral; that allowed us to access and use some facilities during the expeditions.

Funding

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ). JOM has a scholarship from CNPq/UFRRJ (Grant/Award Number: PVBS2480-2021). LASA and CM have scholarships from CAPES (Grant/Award Number: 001). MSO has a post-doctoral scholarship from FAPERJ (Grant/Award Number: E-26/204.228/2021). BPB has fellowships from CNPq (Grant/Award Number: 303899/2019-0) and from FAPERJ (Grant/Award Number: E-26/202.797/2019).

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Authors and Affiliations

  1. Curso de Graduação em Medicina Veterinária, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR-465 km 7, Seropédica, Rio de Janeiro, 23897-000, Brazil

    Jennifer Oliveira Melo

  2. Programa de Pós-Graduação em Biologia Animal, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, BR-465 km 7, Seropédica, Rio de Janeiro, 23897-000, Brazil

    Lucas de Assis Silva Andrade & Mariana de Souza Oliveira

  3. Programa de Pós-Graduação em Ciência, Tecnologia e Inovação em Agropecuária, Universidade Federal Rural do Rio de Janeiro, BR-465 km 7, Seropédica, Rio de Janeiro, 23897-000, Brazil

    Carla Maronezi

  4. Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR-465 km 7, Seropédica, Rio de Janeiro, 23897-000, Brazil

    Ericson Ramos de Mello

  5. Programa de Pós-Graduação em Biomedicina Translacional, Universidade do Grande Rio, Rua Professor José de Souza Herdy 1160, Duque de Caxias, Rio de Janeiro, 25071-202, Brazil

    Sergian Vianna Cardozo

  6. Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Campus Pinheiral, Rua José Breves 550, Pinheiral, Rio de Janeiro, 27197-000, Brazil

    Heider Alves Franco

  7. Departamento de Microbiologia e Imunologia Veterinária, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR-465 km 7, Seropédica, Rio de Janeiro, 23897-000, Brazil

    Águida Aparecida de Oliveira

  8. Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, BR-465 km 7, Seropédica, Rio de Janeiro, 23897-000, Brazil

    Viviane Moreira de Lima & Bruno Pereira Berto

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  1. Jennifer Oliveira Melo
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Correspondence to Bruno Pereira Berto.

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Field-collecting permits were issued by SISBIO/ICMBio (licenses 45200; 49605; 54951; 70132), CEUA/UFRRJ (protocols IV-036/2014; ICBS-008/2015; IV-6606250616) and CEUA/UNIGRANRIO (protocol 021/2019). Banding permits and metal rings were issued by CEMAVE/ICMBio (Senior Ringer: BPB, registration 5967850; Junior Ringer: MSO, registration 7035678). All applicable institutional, national and international guidelines for the care and use of animals were followed.

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Melo, J.O., Andrade, L.A.S., Maronezi, C. et al. Isospora leptopogoni n. sp. (Apicomplexa: Eimeriidae) from the sepia-capped flycatcher Leptopogon amaurocephalus Tschudi, 1846 (Passeriformes: Rhynchocyclidae) in South America. Syst Parasitol 99, 525–534 (2022). https://doi.org/10.1007/s11230-022-10042-8

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