Systematic Parasitology

, Volume 96, Issue 6, pp 453–474 | Cite as

New species and new records of species of Cloacina von Linstow, 1898 (Nematoda: Strongylida) parasitic in the western scrub wallaby, Notamacropus irma (Jourdan) (Marsupialia: Macropodidae) from Western Australia

  • Ian BeveridgeEmail author
  • Abdul Jabbar
  • Anson Koehler
Part of the following topical collections:
  1. Nematoda


The helminth parasites of the western scrub wallaby or black-glove wallaby, Notamacropus irma (Jourdan) which occurs in Western Australia are relatively poorly documented. Six new species of the strongyloid genus Cloacina von Linstow, 1898 (Strongylida: Chabertiidae) are described namely C. asymmetrica n. sp., C. brazellei n. sp., C. harriganae n. sp., C. hobbsi n. sp., C. middletoni n. sp. and C. woodi n. sp. A redescription of C. laius Beveridge, 1999 from the same host species is included. Molecular sequence data (ITS1 and ITS2 ribosomal DNA) were obtained for C. asymmetrica, C. brazellei, C. hobbsi, C. middletoni and from the previously described species C. themis Beveridge, 1998 occurring in the same host species. Phylogenetically, C. asymmetrica, C. hobbsi and C. middletoni formed a distinct clade, suggesting the possibility of within-host speciation. Cloacina themis clustered with a group of morphologically distinctive species in a separate clade and C. brazellei clustered in a third clade but with poor support. This pattern of congeners in a single host species occurring in multiple clades mirrors the situation in other kangaroos and wallabies. Species of Cloacina from N. irma reported thus far therefore consist of a series of species found only in this host, with two species (C. brazellei and C. laius) shared with the sympatric macropodid Setonix brachyurus (Quoy & Gaimard).



Thanks are due to Russ Hobbs and Bob Brazelle for providing material for this study, to Rosemary Harrigan for help in the laboratory and to Shane Middleton for help on field trips. The study was supported financially by the Australian Biological Resources Study (217-06).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable national standards guidelines for the use of animals were followed in this study.


  1. Aussavy, M., Bernardin, E., Corrigan, A., Hufschmid, J., & Beveridge, I. (2011). Helminth parasite communities in four species of sympatric macropodids in western Victoria. Australian Mammalogy, 33, 13–20.CrossRefGoogle Scholar
  2. Beveridge, I. (1998). Taxonomic revision of the genus Cloacina von Linstow (Nematoda: Strongyloidea) from macropodid marsupials. Invertebrate Taxonomy, 12, 273–508.CrossRefGoogle Scholar
  3. Beveridge, I. (1999). New species of Cloacina Linstow, 1898 (Nematoda: Strongyloidea) parasitic in the stomach of the quokka, Setonix brachyurus (Marsupialia: Macropodidae) from Western Australia. Transactions of the Royal Society of South Australia, 123, 17–30.Google Scholar
  4. Beveridge, I. (2002). New species and new records of Cloacina von Linstow, 1898 (Nematoda: Strongyloidea) parasitic in macropodid marsupials from Papua New Guinea. Records of the South Australian Museum, 35, 1–32.Google Scholar
  5. Beveridge, I., Chilton, N. B., & Spratt, D. M. (2002). The occurrence of species flocks in the nematode genus Cloacina (Strongyloidea: Cloacininae) parasitic in the stomachs of kangaroos and wallabies. Australian Journal of Zoology, 50, 597–620.CrossRefGoogle Scholar
  6. Beveridge, I., Jex, A., Tan, N., & Jabbar, A. (2018). New species of Cloacina von Linstow, 1898 (Nematoda: Strongyloidea) parasitic in the stomachs of wallaroos, Osphranter spp. (Marsupialia: Macropodidae) from northern Australia. Systematic Parasitology, 95, 527–542.CrossRefGoogle Scholar
  7. Chabaud, A. G., Puylaert, F., Bain, O., Petter, A. J., & Durette-Desset, M.-C. (1970). Remarques sur lʼ homologie entre les papilles cloacales des Rhabditides et les côtes dorsales des Strongylida. Comptes Rendus Hebdomadaire des Séances de lʼ Académie des Sciences, 271, 1771–1774.Google Scholar
  8. Chilton, N. B. (2004). The use of nuclear ribosomal DNA markers for the identification of bursate nematodes (Order Strongylida) and for the diagnosis of infections. Animal Health Research Reviews, 5, 173–187.CrossRefGoogle Scholar
  9. Chilton, N. B., Shuttleworth, M. A., Huby-Chilton, F., Koehler, A. V., Jabbar, A., Gasser, R. B., et al. (2017). Speciation in the genus Cloacina (Nematoda: Strongylida); species flocks and intra-host speciation. Parasitology, 144, 1828–1840.CrossRefGoogle Scholar
  10. Edgar, R. C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797.CrossRefGoogle Scholar
  11. Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.Google Scholar
  12. Huelsenbeck, J. P., & Ronquist, F. (2001). MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754–755.CrossRefGoogle Scholar
  13. ICZN (2012). International Commission on Zoological Nomenclature: Amendment of articles 8, 9, 10, 21 and 78 of the International Code of Zoological Nomenclature to expand and refine methods of publication. Bulletin of Zoological Nomenclature, 69, 161–169.Google Scholar
  14. Jackson, S., & Groves, C. (2015). Taxonomy of Australian mammals. Clayton South, Australia: CSIRO Publishing, Publishing, 529 pp.CrossRefGoogle Scholar
  15. Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., et al. (2012). Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28, 1647–1649.CrossRefGoogle Scholar
  16. Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33, 1870–1874.CrossRefGoogle Scholar
  17. Poulin, R. (1998). Comparison of three estimators of species richness in parasite component communities. Journal of Parasitology, 84, 485–490.CrossRefGoogle Scholar
  18. Ronquist, F., & Huelsenbeck, J. P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.CrossRefGoogle Scholar
  19. Spratt, D. M., & Beveridge, I. (2016). Helminth parasites of Australasian marsupials and monotremes. Zootaxa, 4123, 1–198.CrossRefGoogle Scholar
  20. Werle, E., Schneider, C., Renner, M., Volker, M., & Fiehn, W. (1994). Convenient single-step, one tube purification of PCR products for direct sequencing. Nucleic Acids Research, 22, 4354–4355.CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Faculty of Veterinary and Agricultural SciencesUniversity of MelbourneParkville, MelbourneAustralia

Personalised recommendations