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Systematic Parasitology

, Volume 95, Issue 5, pp 455–463 | Cite as

Isospora lopesi n. sp. (Apicomplexa: Eimeriidae) from the eastern white-throated spadebill Platyrinchus mystaceus Vieillot (Passeriformes: Tyranni: Tyrannidae) in South America

  • Lidiane Maria da Silva-Carvalho
  • Danilo Giovanni Narciso Pastura
  • Jennifer Vieira Gomes
  • Priscyanne Barreto Siqueira
  • Mariana Borges Rodrigues
  • Viviane Moreira de Lima
  • Bruno Pereira Berto
Article
  • 76 Downloads

Abstract

A species of Isospora Schneider, 1881 (Protozoa: Apicomplexa: Eimeriidae) considered as new to science is described and characterised molecularly from the eastern white-throated spadebill Platyrinchus mystaceus Vieillot in the Parque Nacional do Itatiaia, southeastern Brazil. Isospora lopesi n. sp. has oöcysts that are subspheroidal to ovoidal, 18–24 × 18–22 (20.6 × 19.7) µm, with smooth, bilayered wall, c.1.5 μm thick. Micropyle and oöcyst residuum are absent, but one polar granule is present. Sporocysts are ellipsoidal, 12–16 × 8–11 (14.4 × 8.6) µm. The Stieda body is flattened to half-moon-shaped and sub-Stieda body is rounded. Sporocyst residuum is present, consisting of numerous spherules of different sizes. Sporozoites are vermiform with anterior and posterior refractile bodies and nucleus. Molecular analysis was conducted at the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. This new isolate exhibited similarity greater than 98% with Isospora spp. isolates from spectacled warblers Sylvia conspicillata Temminck, 1820. This is the fourth isosporoid coccidian described from New World tyrannid birds, but is the first to have a complementary molecular characterisation.

Notes

Acknowledgements

We are thankful to the staff at the Parque Nacional do Itatiaia, mainly to the research coordinator Dr Léo Nascimento.

Funding

This study was supported by grants from the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Field collecting permits were issued to BPB by SISBIO/ICMBio (license No. 49605–1) and CEUA/ICBS/UFRRJ (protocol No. 008/2015). All applicable institutional, national and international guidelines for the care and use of animals were followed.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Lidiane Maria da Silva-Carvalho
    • 1
  • Danilo Giovanni Narciso Pastura
    • 2
  • Jennifer Vieira Gomes
    • 2
  • Priscyanne Barreto Siqueira
    • 2
  • Mariana Borges Rodrigues
    • 1
  • Viviane Moreira de Lima
    • 3
  • Bruno Pereira Berto
    • 3
  1. 1.Programa de Pós-Graduação em Ciências VeterináriasUniversidade Federal Rural do Rio de JaneiroSeropédicaBrazil
  2. 2.Curso de Graduação em Ciências BiológicasUniversidade Federal Rural do Rio de JaneiroSeropédicaBrazil
  3. 3.Departamento de Biologia Animal, Instituto de Ciências Biológicas e da SaúdeUniversidade Federal Rural do Rio de JaneiroSeropédicaBrazil

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