Two monorchiid species from the freckled goatfish, Upeneus tragula Richardson (Perciformes: Mullidae), in Moreton Bay, Australia, including a proposal of a new genus
Two monorchiid species are reported from the freckled goatfish, Upeneus tragula Richardson, from Moreton Bay, Queensland, Australia. Specimens of a species new to science were most morphologically similar to species of the genus Timonia Bartoli & Prevot, 1966, but significant differences in the arrangement of the testes (symmetrical vs oblique) and morphology of the terminal organ (bipartite vs unipartite) necessitate the proposal of a new genus; Madhavia n. g. is proposed for M. fellaminutus n. sp. Specimens of the second species are identified as Parachrisomon delicatus (Manter & Pritchard, 1964) Madhavi, 2008, extending its known range from Hawaii to Australia. Complete ITS2 and partial 28S rDNA sequence data were generated for both species and analysed with those for other monorchiids available on GenBank. Phylogenetic analyses of the 28S rDNA dataset showed that both genera are distinct from other sequenced monorchiids, but overall the resolution between genera is poor and more sequence data are required to elucidate relationships within the family. We propose to transfer Timonia stunkardi (Ahmad, 1985) and Timonia vinodae (Ahmad, 1987) to the genus Neotimonia Madhavi, 2008, as Neotimonia stunkardi (Ahmad, 1985) n. comb. and Neotimonia vinodae (Ahmad, 1987) n. comb. Additionally, we were unable to locate any literature on Parachrisomon brotulidorum (Toman, 1973) Madhavi, 2008 and consider this species as nomen nudum.
We sincerely thank Mr John Page, Dave Thompson, Daniel Huston, Russell Yong and Storm Martin for their assistance with the collection of fish in Moreton Bay and the staff of the Moreton Bay Research Station for their support of our work.
TC and SC acknowledge the Australian Biological Resources Study for its ongoing support. This work was supported by an ABRS grant to explore the parasites of fishes of Moreton Bay (RF215-40). NW is supported by a PhD scholarship from the University of Queensland (Research Training Program (RTP) Scholarship).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This study was conducted in compliance with all institutional, national and international guidelines on the care and use of animals.
- Ahmad, J. (1985). Digenetic trematodes of marine fishes from the Arabian Sea. Part 43. Pakistan Journal of Zoology, 17, 147–164.Google Scholar
- Atopkin, D. M., Besprozvannykh, V. V., Ngo, H. D., Van Ha, N., Van Tang, N., Ermolenko, A. V., et al. (2017). Morphometric and molecular data of the two digenean species Lasiotocus lizae Liu, 2002 (Monorchiidae) and Paucivitellosus vietnamensis sp. n. (Bivesiculidae) from mullet fish in Tonkin Bay, Vietnam. Journal of Helminthology, 91, 346–355.CrossRefPubMedGoogle Scholar
- Bagnato, E., Gilardoni, C., Pina, S., Rodrigues, P., & Cremonte, F. (2016). Redescription and life cycle of the monorchiid Postmonorcheides maclovini Szidat, 1950 (Digenea) from the Southwestern Atlantic Ocean: Morphological and molecular data. Parasitology International, 65, 44–49.CrossRefPubMedGoogle Scholar
- Bartoli, P., & Bray, R. A. (2004). Ancylocoelium typicum Nicoll, 1912 (Digenea: Monorchiidae), a poorly known parasite of Trachurus spp. (Teleostei: Carangidae) from the western Mediterranean and north-eastern Atlantic, and observations on its taxonomic position. Systematic Parasitology, 58, 23–29.CrossRefPubMedGoogle Scholar
- Bray, R. A., Justine, J.-L., & Cribb, T. H. (2009). Cableia balistidicola n. sp. (Digenea, Monorchiidae) from Pacific Ocean balistids (Tetraodontiformes) and new reports of Cableia pudica Bray, Cribb and Barker, 1996 in temperate Australian monacanthids. Parasitology International, 58, 341–345.CrossRefPubMedGoogle Scholar
- Cutmore, S. C., Diggles, B. K., & Cribb, T. H. (2016). Transversotrema Witenberg, 1944 (Trematoda: Transversotrematidae) from inshore fishes of Australia: Description of a new species and significant range extensions for three congeners. Systematic Parasitology, 93, 639–652.CrossRefPubMedGoogle Scholar
- ICZN (2012). International Commission on Zoological Nomenclature: Amendment of articles 8, 9, 10, 21 and 78 of the International Code of Zoological Nomenclature to expand and refine methods of publication. Bulletin of Zoological Nomenclature, 69, 161–169.Google Scholar
- Littlewood, D. T. J., Rohde, K., & Clough, K. A. (1997). Parasite speciation within or between host species? - Phylogenetic evidence from site-specific polystome monogeneans. Parasitology, 27, 1289–1297.Google Scholar
- Maddison, W. P., & Maddison, D. R. (2017). Mesquite: A modular system for evolutionary analysis. Version, 3, 2.Google Scholar
- Sambrook, J., & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.Google Scholar
- Tkach, V. V., Pawlowski, J., Mariaux, J., & Swiderski, Z. (2001). Molecular phylogeny of the suborder Plagiorchiata and its position in the system of Digenea. In: Littlewood, D. T. J. & Bray, R. A. (Eds), Interrelationships of Platyhelminthes. London: Taylor & Francis, pp. 186–193.Google Scholar
- Toman, G. (1973). Two new digenetic trematodes, Paramonorcheides brotulidorum n. sp. (Monorchiidae) and Dinosoma lophiomi n. sp. (Hemiuridae), from deep sea fishes from Japan. Research Bulletin of the Meguro Parasitological Museum, 7, 4–7.Google Scholar
- WoRMS. (2018). Monorchiidae Odhner, 1911. http://www.marinespecies.org/aphia.php?p=taxdetails&id=108453. Accessed 4 March 2018.
- Yamaguti, S. (1970). Digenetic Trematodes of Hawaiian Fishes. Tokyo: Keigaku Publishing.Google Scholar