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Two new species of Haplorchoides Chen, 1949 (Digenea: Heterophyidae) infecting an Australian siluriform fish, Neoarius graeffei Kner & Steindachner

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Abstract

Combined morphological and molecular analyses are used to characterise two new species of Haplorchoides Chen, 1949 (Digenea: Heterophyidae) from an Australian siluriform fish. Haplorchoides maiwariensis n. sp. and H. daguilarensis n. sp. are described from the intestine of the Blue salmon catfish, Neoarius graeffei (Kner & Steindachner) (Siluriformes: Ariidae) from tidal reaches of the Brisbane River and from a freshwater creek off Lake Wivenhoe, Kipper Creek, Australia. The two new species most obviously differ from all previously described species of Haplorchoides in possessing clearly and reliably tripartite seminal vesicles. The two new species differ in the size and distribution of vitelline follicles, the size of the pigment granules and the form of the ventral sucker. Previously described species of Haplorchoides have been reported from Africa and Asia, principally from bagrid, schilbeid, silurid and sisorid catfishes; these are the first species reported from an ariid catfish. Complete ITS2 and partial 28S ribosomal DNA data were generated for both new species. The two species differ from each other by eight base pairs in the ITS2 region, and by 13 bp for the 28S region. The 28S rDNA sequence of H. daguilarensis agrees with a previously reported sequence from an unidentified species of Haplorchoides collected from N. graeffei in Lake Wivenhoe, Australia; we identify this previous report as relating to H. daguilarensis.

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Acknowledgements

We sincerely thank Ms Hoi Yan Iao, Mr Nicholas Q-X. Wee and Mr Yat Long Angus Li for their assistance with the collection of fish specimens from the Brisbane River and Lake Wivenhoe, and Mr Arnault Gauthier for providing the fishing location at Lake Wivenhoe.

Funding

THC and SCC acknowledge the Australian Biological Resources Study (ABRS) for their ongoing support. This study was funded by the ABRS National Taxonomy Research Grant RF215-40.

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  1. School of Biological Sciences, The University of Queensland, St. Lucia, QLD, 4067, Australia

    Rémy Hostettler, Scott C. Cutmore & Thomas H. Cribb

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  1. Rémy Hostettler
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  2. Scott C. Cutmore
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  3. Thomas H. Cribb
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Correspondence to Rémy Hostettler.

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This article was registered in the Official Register of Zoological Nomenclature (ZooBank) as urn:lsid:zoobank.org:pub:5C476750-0DE3-4A25-9E36-3CFEF76685CD. This article was published as an Online First article on the online publication date shown on this page. The article should be cited using the doi number. This is the version of record.

This article is part of the Topical Collection Digenea.

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Hostettler, R., Cutmore, S.C. & Cribb, T.H. Two new species of Haplorchoides Chen, 1949 (Digenea: Heterophyidae) infecting an Australian siluriform fish, Neoarius graeffei Kner & Steindachner. Syst Parasitol 95, 201–211 (2018). https://doi.org/10.1007/s11230-018-9775-3

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