Skip to main content

Two new species of coccidia (Apicomplexa: Eimeriidae) from leaf-tailed geckos, Uroplatus spp. (Sauria: Gekkonidae) from Madagascar, including a new host of Eimeria brygooi Upton & Barnard, 1987

Abstract

During May and June 2015, four common leaf-tailed geckos, Uroplatus fimbriatus (Schneider), five satanic leaf-tailed geckos, Uroplatus phantasticus (Boulenger), and four mossy leaf-tailed geckos, Uroplatus sikorae Boettger originally collected from Madagascar and housed at the Dallas Zoo, USA, had their faeces examined for coccidian parasites. Eight (62%) geckos were found to be passing oöcysts, including a new eimerian, a new isosporan and a previously described eimerian. Three of four (75%) U. fimbratus (type-host) and one of five (20%) U. phantasticus were infected with Eimeria schneideri n. sp.; oöcysts were subspheroidal to ellipsoidal with a bi-layered wall and measured (mean length × width, L × W) 15.1 × 13.5 µm, with a length/width (L/W) ratio of 1.1. A micropyle and oöcyst residuum were absent but one to many polar granules were present. Sporocysts were ovoidal, 6.9 × 5.3 µm, L/W = 1.3. Stieda, sub-Stieda and para-Stieda bodies were absent. A globular sporocyst residuum was present as dispersed granules. Four of five (80%) U. phantasticus harboured Isospora boulengeri n. sp.; oöcysts were subpheroidal to ellipsoidal with a bi-layered wall and measured 17.3 × 16.0 µm, L/W = 1.1. A micropyle and oöcyst residuum were absent but a polar granule was present. Sporocysts were ellipsoidal, 9.5 × 6.9 µm, L/W = 1.4. Stieda and sub-Stieda bodies were present but a para-Stieda body was absent. A globular sporocyst residuum was present with dispersed granules. In addition, one of four (25%) U. sikorae was infected with an eimerian indistinguishable from Eimeria brygooi Upton & Barnard, 1987, previously reported from Madagascar day gecko, Phelsuma grandis Gray and golddust day gecko, Phelsuma laticauda (Boettger) from Madagascar. These are the first coccidians described from Uroplatus spp.

This is a preview of subscription content, access via your institution.

Fig. 1–11
Fig. 12–13

References

  1. Abdel-Baki, A. S., Al-Quraishy, S., Al Otaibi, M. S., & Duszynski, D. W. (2013). A new species of Isospora (Apicomplexa: Eimeriidae) infecting the Baiuch rock gecko, Bunopus tuberculatus, in Saudi Arabia. Journal of Parasitology, 99, 1019–1023.

    CAS  Article  PubMed  Google Scholar 

  2. Alnutt, T. F., Powell, G. V. N., Ferrier, S., Ricketts, T. H. Steininger, M. K., & Manion, G. (2004). Quantifying biodiversity loss in Madagascar using biological inventories, environmental data and a 50-year record of deforestation. Society for Conservation Biology meetings, New York, 30 July-2 August 2004.

  3. Berto, B. P., Lopes, B. Do, Flausino, W., Teixeira-Filho, W. L., & Lopes, C. W. G. (2008). Contribution on the study of Isospora hemidactyli Carini, 1936 and a report of an adeleid pseudoparasite of the house gecko Hemidactylus mabouia, from the Rio de Janeiro metropolitan region, Brazil. Revista Brasileira de Parasitologia Veterinária, 17, 150–154.

    PubMed  Google Scholar 

  4. Berto, B. P., McIntosh, D., & Lopes, C. W. G. (2014). Studies on coccidian oocysts (Apicomplexa: Eucoccidiorida). Revista Brasileira de Parasitologia Veterinária, 23, 1–15.

    Article  PubMed  Google Scholar 

  5. Brygoo, E. R. (1963). Contribution à la connaissance de la parasitologie des Caméléons Malgaches (2e partie). Annales de Parasitologie Humaine et Comparée, 38, 525–739.

    CAS  PubMed  Google Scholar 

  6. Carini, A. (1936). Sur une Isospora de l’intestin de l’Hemidactylus mabuaje. Annales de Parasitologie Humaine et Comparée, 14, 444–446.

    Google Scholar 

  7. Daszak, P., & Ball, S. J. (1991). Five new species of Eimeria (Apicomplexa: Eimeriidae) from lizards. Systematic Parasitology, 20, 141–147.

    Article  Google Scholar 

  8. El-Touhky, A. A., Abdel-Aziz, A., Abo-Senna, F. M., & El-Nour, M. F. (2013). Three coccidian parasites from Moorish gecko, Tarentola mauritanica (Gekkonidae) 2-Eimeria alexandriensis n. sp. (Apicomplexa: Eimeriidae). International Journal of Advanced Research, 1, 526–534.

    Google Scholar 

  9. Glaw, F., & Vences, M. (2007). A field guide to the amphibians and reptiles of Madagascar (3rd ed). Cologne: Vences and Glaw Verlag, 496 pp.

  10. Greenbaum, E., Bauer, A., Jackman, T., Vences, M., & Glaw, F. (2007). A phylogeny of the enigmatic Madagascan geckos of the genus Uroplatus. Zootaxa, 1493, 41–51.

    Google Scholar 

  11. Jirků, M., Modrý, D., Šlapeta, J. R., Koudela, B., & Lukeš, J. (2002). The phylogeny of Goussia and Choleoeimeria (Apicomplexa: Eimeriorina) and the evolution of excystation structures in coccidia. Protist, 153, 380–389.

    Google Scholar 

  12. Lainson, R., & Paperna, I. (1999). Re-descriptions of Isospora ameivae Carini, 1932 in the teiid lizard Ameiva ameiva and Isospora hemidactyli Carini, 1936 in the gecko Hemidactylus mabouia, with particular reference to their endogenous stages. Memórias do Instituto Oswaldo Cruz, 94, 459–466.

    CAS  Article  PubMed  Google Scholar 

  13. Leinwand, I., Kilpatrick, A. M., Cole, N., Jones, C. G., & Daszak, P. (2005). Patterns of coccidial prevalence in lizards of Mauritius. Journal of Parasitology, 91, 1103–1108.

    Article  PubMed  Google Scholar 

  14. Matuschka, F.-R., & Bannert, B. (1986). Eimeria tarentolae new species from the Moorish gecko, Tarentola mauritanica. Journal of Protozoology, 33, 309–311.

    Article  Google Scholar 

  15. McAllister, C. T. (2012). A new species of Choleoeimeria (Apicomplexa: Eimeriidae) from Oustalet’s chameleon, Furcifer oustaleti (Sauria: Chamaeleonidae). Folia Parasitologica, 59, 12–14.

    Article  PubMed  Google Scholar 

  16. Megía-Palma, R., Martínez, J., Acevedo, I., Martín, J., García-Roa, R., Ortega, J., et al. (2015). Phylogeny of the reptilian Eimeria: Are Choleoeimeria and Acroeimeria valid names. Zoologica Scripta, 44, 684–692.

    Article  Google Scholar 

  17. Modrý, D. (1998). The biology and pathogenicity of caryosporan coccidia. Ph.D. dissertation, Department of Parasitology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. 52 p. + xiv Appendices.

  18. Modrý, D., Daszak, P., Volf, J., Veselý, M., Ball, S. J., & Koudela, B. (2001). Five new species of coccidia (Apicomplexa: Eimeriidae) from Madasgascan chameleons (Sauria: Chamaeleonidae). Systematic Parasitology, 48, 117–123.

    Article  PubMed  Google Scholar 

  19. Myers, N., Mittermeier, R. A., Mittermeier, C. G., da Fonseca, G. A. B., & Kent, J. (2000). Biodiversity hotspots for conservation priorities. Nature, 403, 853–858.

    CAS  Article  PubMed  Google Scholar 

  20. Paperna, I., & Landsberg, J. H. (1989). Description and taxonomic discussion of eimerine coccidia from African and Levantine geckoes. South African Journal of Zoology, 24, 335–345.

    Google Scholar 

  21. Ratsoavina, F. M., Raminosoa, N. R., Louis, E. E, Jr., Raselimanana, A. P., Glaw, F., & Vences, M. (2013). An overview of Madagascar’s leaf tailed geckos (genus Uroplatus): species boundaries, candidate species, and review of geographical distribution based on molecular data. Salamandra, 49, 115–148.

    Google Scholar 

  22. Raxworthy, C. J., & Nussbaum, R. A. (1994). A rainforest survey of amphibians, reptiles and small mammals at Montagne D’Ambre, Madagascar. Biological Conservation, 69, 65–73.

    Article  Google Scholar 

  23. Raxworthy, C. J., Pearson, R. G., Zimkus, B. M., Reddy, S., Deo, A. J., Nussbaum, R. A., et al. (2008). Continental speciation in the tropics: contrasting biogeographic patterns of divergence in the Uroplatus leaf-tailed gecko radiation of Madagascar. Journal of Zoology, 275, 423–440.

    Article  Google Scholar 

  24. Scantelbury, D. P. (2013). Diversification rates have declined in the Malagasy herpetofauna. Proceedings of the Royal Society B, 280, 1109.

    Google Scholar 

  25. Steininger, M., Harper, G., Juhn, D., & Hawkins, F. (2003). Analyse de changment de couverture forestière nationale 1990-2000. Washington, DC: CI Center for Applied Biodiversity Science.

    Google Scholar 

  26. Tanabe, M. (1928). Eimeria gekkonis n. sp., a new coccidium from the gecko, Gecko japonicus (Duméril et Bibron). Journal of the Chosen Medical Association, 87, 65–69.

    Google Scholar 

  27. Uetz, P., & Hošek, J. (2016). The TIGR Reptile Database. World Wide Web electronic publication. http://www.reptile-database.org/. Accessed 23 March 2016.

  28. Upton, S. J., & Barnard, S. M. (1987). Two new species of coccidia (Apicomplexa: Eimeriidae) from Madagascar gekkonids. Journal of Protozoology, 34, 452–454.

    CAS  Article  PubMed  Google Scholar 

  29. Upton, S. J., Freed, P. S., Burdick, D. A., & McAllister, C. T. (1990). Seven new species of coccidians (Apicomplexa: Eimeriorina) from reptiles in Madagascar. Canadian Journal of Zoology, 69, 2368–2375.

    Article  Google Scholar 

  30. Upton, S. J., Hanley, J. K., Case, T. J., & McAllister, C. T. (1991). Description of Isospora schlegeli sp. nov. (Apicomplexa: Eimeriidae) from gekkonid lizards in the South Pacific. Canadian Journal of Zoology, 69, 3108–3110.

    Article  Google Scholar 

  31. Wolf, D., Vrhovec, M. G., Failing, K., Rossier, C., Hermosilla, C., & Pantchev, N. (2014). Diagnosis of gastrointestinal parasites in reptiles: comparison of two coprological methods. Acta Veterinaria Scandinavica, 56, 1–13.

    Article  Google Scholar 

Download references

Acknowledgements

We thank Drs S.L. Gardner (HWML) and S.E. Trauth (ASUMZ) for expert curatorial assistance. We also thank Dr. D. Modrý (Brno, Czech Republic) for providing some information from his unpublished dissertation.

Funding

This publication was made possible in part by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under Grant # 2P20GM103432. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NIH.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Chris T. McAllister.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable institutional, national and international guidelines for the care and use of animals were followed.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

McAllister, C.T., Scott Seville, R. & Hartdegen, R. Two new species of coccidia (Apicomplexa: Eimeriidae) from leaf-tailed geckos, Uroplatus spp. (Sauria: Gekkonidae) from Madagascar, including a new host of Eimeria brygooi Upton & Barnard, 1987. Syst Parasitol 93, 815–823 (2016). https://doi.org/10.1007/s11230-016-9660-x

Download citation

Keywords

  • Compact Mass
  • Polar Granule
  • Refractile Body
  • Coccidian Parasite
  • Stieda Body