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A new genus and two new nematode species (Drilonematoidea: Ungellidae: Synoecneminae) parasitic in two morphs of Drawida ghilarovi Gates, endemic earthworm from the Russian Far East

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Abstract

Drasico n. g. is erected to accommodate two new species of nematode, Drasico nemoralis n. sp. and D. paludigenus n. sp., recovered from coelomic cavities of Drawida ghilarovi Gates, endemic earthworms of the Russian Far East. The new genus is characterised by the following unique for the Synoecneminae characters: apical portion of the head attenuated, cephalic hooks displaced to the base of attenuated portion, amphids displaced posterior to cephalic hooks, excretory duct short and weak, males possessing several genital papilliform sensilla. The new species are differentiated by the size, number and disposition of the male genital sensilla (larger and more numerous in D. nemoralis n. sp.); the body shape of females (with thinner neck and wider mid-body in D. paludigenus n. sp.) and the ovarian tube arranged in transversal folds in D. paludigenus (vs longitudinal folds in D. nemoralis n. sp.). Nucleotide sequences of D2–D3 expansion segment of 28S rDNA for the two new species differed at 13 positions. Phylogenetic analysis revealed close relationships of Drasico n. g. with species of Siconema Timm, 1966. The host species was represented by two morphs (blue-grey forest and tar-black meadow-swamp morph) with intraspecific divergence of 16–17% for cytochrome c oxidase subunit 1 (COI) gene, and each host morph was found infected by a different nematode species. A co-infection with the plectid nematode Creagrocercus drawidae Ivanova & Spiridonov, 2011 was recorded together with D. nemoralis n. sp. in the blue-grey forest morph.

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References

  • Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215, 403–410.

    CAS  PubMed  Google Scholar 

  • Anonymous (2001). [Red Data Book of the Russian Federation: Animals]. AST & Astrel Publishers. 863 pp (In Russian).

  • Anonymous (2008). [Red Book of Khabarovsky Krai]. Khabarovsk: Priamurskiye Vedomosti, 632 pp (In Russian).

  • Berman, D. I., Mescheryakova, E. N., Leirikh, A. N., & Kurenshchikov, D. K. (2010). Geographic range and cold hardiness of the earthworm Drawida ghilarovi (Oligochaeta, Moniligastridae). Biology Bulletin, 3, 895–904.

    Article  Google Scholar 

  • Blakemore, R. J. (2003). Japanese earthworms (Annelida: Oligochaeta): a review and checklist of species. Organisms, Diversity & Evolution, 3, 241–244.

    Article  Google Scholar 

  • Blakemore, R. J. (2007). Checklist of megadrile earthworms (Annelida: Oligochaeta) from mainland China plus Hainan Island. http://www.annelida.net/earthworm/China.pdf. Accessed December, 2013.

  • Blakemore, R. J., & Kupriyanova, E. K. (2010). Unraveling some Kinki worms (Annelida: Oligochaeta: Megadrili: Moniligastridae) - Part I. Opuscula Zoologica Budapest, 41, 3–18.

    Google Scholar 

  • Blakemore, R. J., Kupriyanova, E. K., & Grygier, M. J. (2010). Neotypification of Drawida hattamimizu Hatai, 1930 (Annelida, Oligochaeta, Megadrili, Moniligastridae) as a model linking mtDNA (COI) sequences to an earthworm type, with a response to the ‘Can of Worms’ theory of cryptic species. ZooKeys, 41, 1–29.

    Article  Google Scholar 

  • Coleman, Ch. O. (2003). “Digital inking”: How to make perfect line drawings on computers. Organisms, Diversity & Evolution 3 (Electronic Supplement), 14, 1–14.

  • Ganin, G. N. (1997). [Soil invertebrates of Ussury taiga (Priamurye and Primorye of Russian Far East)]. Vladivostok-Khabarovsk: Dal’nauka. 160 pp (in Russian).

  • Ganin, G. N., Anisimov, A. P., Atopkin, D. M., & Roslick, G. V. (2012). [Molecular and cytogenetic studies of Drawida ghilarovi Gates, 1969 (Oligochaeta, Moniligastridae) – the Russian Far East endemic.] [“Modern problems of regional development”. Materials of IV International conference. Birobidzhan, 9–12 October 2012. Institute of Complex Analysis of Regional Problems FEB RAN], p. 123 (in Russian).

  • Huelsenbeck, J. P., & Ronquist, F. (2001). MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754–755.

    Article  CAS  PubMed  Google Scholar 

  • Ivanova, E. S. (1993). I. Four new species of Dicelis Dujardin, 1845 from Eastern Europe and a diagnosis of the genus. Russian Journal of Nematology, 1, 103–116.

    Google Scholar 

  • Ivanova, E. S. (1994). II. Two new species of Dicelis Dujardin, 1845 from Siberia and a key to the species of the genus. Russian Journal of Nematology, 2, 65–74.

    Google Scholar 

  • Ivanova, E. S., & Neuhaus, B. (2009). Malvinia crassa gen. n., sp. n. (Nematoda, Drilonematoidea) – a parasite of the acanthodrilid earthworm, Notodrilus bovei, from the Falkland Islands. Nematology, 11, 39–45.

    Article  Google Scholar 

  • Ivanova, E. S., & Spiridonov, S. E. (2011). Two new species of creagrocercid nematodes parasitic in earthworms, with comments on the phylogenetic affiliations of the Creagrocercidae Baylis, 1943. Systematic Parasitology, 78, 81–94.

    Article  CAS  PubMed  Google Scholar 

  • Ivanova, E. S., Pham Van Luc, & Spiridonov, S. E. (2012). Two new species of Siconema (Drilonematoidea: Ungellidae) parasitic in earthworms in Vietnam, and systematic relationships as inferred from ribosomal sequence data. Zootaxa, 3344, 47–59.

    Google Scholar 

  • Jamieson, B. G. M. (1977). On the phylogeny of the Moniligastridae, with description of a new species of Moniligaster (Oligochaeta, Annelida). Evolutionary Theory, 2, 95–114.

    Google Scholar 

  • Poinar, G. O., Jr. (1978). Associations between nematodes (Nematoda) and oligochaetes (Annelida). Proceedings of the Helminthological Society of Washington, 45, 202–210.

    Google Scholar 

  • Seinhorst, J. W. (1959). A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica, 4, 54–60.

    Google Scholar 

  • Spiridonov, S. E., & Ivanova, E. S. (2005). [The nematodes of the superfamily Drilonematoidea, parasites of earthworms.] Moscow: Tovarischestvo nauchnyh izdaniy KMK, 296 pp (In Russian).

  • Spiridonov, S. E., Ivanova, E. S., & Pham Van Luc (2007). Two new species of Ungellidae and Homungellidae (Drilonematoidea; Rhabditida) from Vietnamese earthworms and the phylogenetic links of these families. Russian Journal of Nematology, 15, 101–108.

    Google Scholar 

  • Spiridonov, S. E., Ivanova, E. S., & Wilson, M. J. (2005). The nematodes of the genus Dicelis Dujardin, 1845 parasitic in earthworms: the interrelationships of four Eurasian populations. Russian Journal of Nematology, 13, 61–81.

    Google Scholar 

  • Swofford, D. L. (1998). PAUP*. Phylogenetic analysis using parsimony. Version 4. Sinauer, Sunderland, MA.

  • Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739.

    Article  CAS  PubMed  Google Scholar 

  • Timm, R. W. (1959). Observations on Synoecnema (Nematoda, Ungellidae) with a description of 2 new species. Pakistan Journal of Scientific Research, 11, 58–62.

    Google Scholar 

  • Timm, R. W. (1966a). Nematode parasites of the coelomic cavity of earthworms. IV. Siconema new genus (Drilonematoidea: Ungellidae) with a description of 10 new species. Biologia (Dacca), 12, 7–21.

    Google Scholar 

  • Timm, R. W. (1966b). Nematode parasites of the coelomic cavity of earthworms. VI. Macramphida and Sucamphida, two new genera with unusual amphids new species. Proceedings of the Helminthological Society of Washington, 33, 67–71.

    Google Scholar 

  • Timm, R. W. (1967). Nematode parasites of the coelomic cavity of earthworms. VIII. Four new genera and seven new species of the family Ungellidae. Pakistan Journal of Scientific Research, 10, 13–21.

    Google Scholar 

  • Vsevolodova-Perel, T.S. (1997). [The Earthworms of the Fauna of Russia. Cadaster and Key.] Moscow, “Nauka”, 99 pp (In Russian).

  • Yeates, G. W., & Spiridonov, S. E. (1996). New nematodes of the families Drilonematidae, Ungellidae, and Mesidionematidae from New Zealand megascolecid earthworms. New Zealand Journal of Zoology, 23, 381–399.

    Article  Google Scholar 

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Acknowledgements

E. Ivanova and S. Spiridonov acknowledge the support by a grant from the Russian Fund for Basic Research 14-04-01513.

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Authors and Affiliations

  1. Centre of Parasitology of the A. N. Severtsov Institute of Ecology and Evolution RAS, Leninskii Prospect 33, 119071, Moscow, Russia

    Elena S. Ivanova & Sergei E. Spiridonov

  2. Institute for Water and Ecological Problems, FEB RAS, Khabarovsk, Russia

    Gennadiy N. Ganin

Authors
  1. Elena S. Ivanova
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  2. Gennadiy N. Ganin
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  3. Sergei E. Spiridonov
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Correspondence to Elena S. Ivanova.

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Ivanova, E.S., Ganin, G.N. & Spiridonov, S.E. A new genus and two new nematode species (Drilonematoidea: Ungellidae: Synoecneminae) parasitic in two morphs of Drawida ghilarovi Gates, endemic earthworm from the Russian Far East. Syst Parasitol 87, 231–248 (2014). https://doi.org/10.1007/s11230-014-9471-x

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  • DOI: https://doi.org/10.1007/s11230-014-9471-x

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