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Systematic Parasitology

, Volume 81, Issue 2, pp 115–123 | Cite as

Morphological and molecular study of the poorly known species Pseudanisakis rajae (Yamaguti, 1941) (Nematoda: Acanthocheilidae) from elasmobranchs in the Yellow Sea and Taiwan Strait off the coast of China

  • Liang Li
  • David I. Gibson
  • Yuan-Yuan Liu
  • Lu-Ping Zhang
Article

Abstract

Ascaridoid nematodes identified as Pseudanisakis rajae (Yamaguti, 1941) were collected from the skates Bathyraja smirnovi (Soldatov & Pavlenko), Okamejei kenojei (Müller & Henle) and Raja pulchra Liu (Rajiformes: Rajidae) in the Yellow Sea and Taiwan Strait off the coast of China. Their examination using light microscopy and, for the first time, scanning electron microscopy revealed erroneous and previously unreported morphological features, necessitating the redescription of this little known species. In addition, specimens of P. rajae collected from the three different hosts were characterised using molecular methods by sequencing and analysing the internal transcribed spacer (ITS) of the ribosomal DNA. These new morphological and molecular data enabled an updated diagnosis of this nematode and the presentation of an identification key to the species of Pseudanisakis Layman & Borovkova, 1926.

Keywords

Internal Transcribe Spacer Excretory Pore Ejaculatory Duct Anterior Extremity Spiral Intestine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors wish to thank Professor Rui-Yong An (College of Life Sciences, Hebei Normal University) for identifying the fishes. This study was supported by the National Natural Science Foundation of China (NSFC–30970318, 31101615) and a grant (No. L2010B13) from the Hebei Normal University to Dr Liang Li.

References

  1. D’Amelio, S., Mathiopoulos, K. D., Santos, C. P., Pugachev, O. N., Webb, S. C., Picanco, M., & Paggi, L. (2000). Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. International Journal for Parasitology, 30, 223–226.PubMedCrossRefGoogle Scholar
  2. de Rodrigues, H. O., Carvalho Varela, M., Sodre Rodrigues, S., & Cristofaro, R. (1973). Alguns nematódeos de peixes do Oceano Atlântico – Costa continental portuguesa e Costa do Norte da Africa. Memorias do Instituto Oswaldo Cruz, 71, 247–256.CrossRefGoogle Scholar
  3. Du, C.-X., Zhang, L.-P., Shi, M.-Q., Ming, Z., Hu, M., & Gasser, R. B. (2010). Elucidating the identity of Anisakis larvae from a broad range of marine fishes from the Yellow Sea, China, using a combined electrophoretic-sequencing approach. Electrophoresis, 31, 654–658.PubMedCrossRefGoogle Scholar
  4. Fang, W.-Z., Xu, S.-S., Zhang, S.-L., Wang, Y.-N., Chen, X.-B., & Luo, D.-M. (2010). Multiple primer PCR for the identification of anisakid nematodes from Taiwan Strait. Experimental Parasitology, 124, 197–201.PubMedCrossRefGoogle Scholar
  5. Gibson, D. I. (1973). The genus Pseudanisakis Layman & Borovkova, 1926 (Nematoda: Ascaridida). Journal of Natural History, 7, 319–340.CrossRefGoogle Scholar
  6. Hsü, H.-F. (1933). On some species of parasitic nematodes from fishes in China. Peking Natural History Bulletin, 8, 147–154.Google Scholar
  7. Layman, E. M., & Borovkova, M. M. (1926). [Parasitic worms of the skate (Raja radiate Don.) from material of the 15th Federal Helminthological Expedition.] Raboty Parazitologicheskoi Laboratorii. Moskovskii Gosudarstvennyi Universitet, pp. 9–26 (In Russian).Google Scholar
  8. Li, L., Xu, Z., & Zhang, L.-P. (2007a). A new species of genus Hysterothylacium Ward et Magath, 1917 (Nematoda, Anisakidae) from Liparis tanakae (Scorpaeniformes, Liparidae) from the Yellow Sea, China. Acta Parasitologica, 52, 371–375.CrossRefGoogle Scholar
  9. Li, L., An, R.-Y., & Zhang, L.-P. (2007b). A new species of Hysterothylacium (Nematoda: Anisakidae) from marine fishes from Yellow Sea, China, with a key to the species of the genus Hysterothylacium. Zootaxa, 1614, 43–52.Google Scholar
  10. Li, L., Xu, Z., & Zhang, L.-P. (2008). Redescription of three species of Hysterothylacium (Nematoda: Anisakidae) from marine fishes from the Yellow Sea, China, with the synonymy of Hysterothylacium muraenesoxin (Luo, 1999). Zootaxa, 1878, 55–67.Google Scholar
  11. Luo, D.-M. (2001). Notes on nematodes of fishes from Taiwan Strait. II. (Nematoda: Spiruridea; Ascarididea). Acta Zootaxonomica Sinica, 26, 281–288 (In Chinese, English summary).Google Scholar
  12. Luo, D.-M., & Huang, H. (2001). Heliconema minnanensis n. sp. (Physalopteroidea: Physalopteridae) and Raphidascaris trichiuri (Yin and Zhang) n. comb. (Ascaridoidea: Anisakidae) in marine fishes. Journal of Parasitology, 87, 1090–1094.Google Scholar
  13. Mattiucci, S., & Nascetti, G. (2008). Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite coevolutionary processes. Advances in Parasitology, 66, 47–148.PubMedCrossRefGoogle Scholar
  14. Mosgovoi, A. A. (1950). [Notes on the foundation of the genus Pseudanisakis (Layman & Borovkova, 1926) nov. gen.] Trudy Gel’mintologicheskoi Laboratorii, 4, 17–24. (In Russian).Google Scholar
  15. Santos, C. P., Lent, H., & Gibson, D. I. (2004). A new species of Pseudanisakis Layman & Borovkova, 1926 (Nematoda: Ascaridida) from Rioraja agassizii and Psammobatis extenta (Rajidae) in Brazilian southwestern Atlantic waters. Systematic Parasitology, 57, 229–235.PubMedCrossRefGoogle Scholar
  16. Testini, G., Papini, R., Lia, R. P., Parisi, A., Dantas-Torres, F., Traversa, D., & Otranto, D. (2011). New insights into the morphology, molecular characterization and identification of Baylisascaris transfuga (Ascaridida, Ascarididae). Veterinary Parasitology, 175, 97–102.PubMedCrossRefGoogle Scholar
  17. Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.PubMedCrossRefGoogle Scholar
  18. Wang, P.-Q. (1965). Notes on some nematodes of the Suborder Ascaridata from Fukien, China. Acta Parasitologica Sinica, 2, 366–379 (In Chinese, English summary).Google Scholar
  19. Wang, P.-Q. (1984). Some nematodes of fishes from Fujian Province, China. Acta Zootaxonomica Sinica, 9, 228–237 (In Chinese, English summary).Google Scholar
  20. Williams, H. H., & Richards, D. H. H. (1968). Observations on Pseudanisakis rotundata (Rudolphi, 1819) Mosgovoi, 1950, a common but little known nematode parasite of Raia radiata Donovan in the northern North Sea. Journal of Helminthology, 42, 199–220.CrossRefGoogle Scholar
  21. Wu, H.-W. (1927). A new nematode from the stomach of a scylloid shark. Contributions from the Biological Laboratory of the Science Society of China, 3, 1–3.Google Scholar
  22. Wu, H.-W. (1949). A note on two parasitic nematodes of fishes. Sinensia, 20, 51–57.Google Scholar
  23. Yamaguti, S. (1941). Studies on the helminth fauna of Japan, Part 33. Nematodes of Fishes. II. Japanese Journal of Zoology, 9, 343–396.Google Scholar
  24. Yin, W.-Z. (1983). On some nematodes from marine fishes in Hainan Islands, China. Acta Zootaxonomica Sinica, 8, 225–228.Google Scholar
  25. Yin, W.-Z., & Zhang, N.-X. (1983a). On two new species of parasitic nematodes from marine fishes from Zhoushan Islands, China. Acta Zootaxonomica Sinica, 8, 7–10.Google Scholar
  26. Yin, W.-Z., & Zhang, N.-X. (1983b). Notes on some nematodes of marine fishes from Fujian, China. Acta Zootaxonomica Sinica, 8, 11–16 (In Chinese, English summary).Google Scholar
  27. Zhang, L.-P., Hu, M., Shamsi, S., Beveridge, I., Li, H.-M., Xu, Z., Li, L., Cantacessi, C., Gasser, R.B. (2007). The specific identification of anisakid larvae from fishes from the Yellow Sea, China, using mutation scanning-coupled sequence analysis of nuclear ribosomal DNA. Molecular and Cellular Probes, 21, 386–390 (In Chinese, English summary).PubMedCrossRefGoogle Scholar
  28. Zhu, X.-Q., Podolska, M., Liu, J.-S., Yu, H.-Q., Chen, H.-H., Lin, Z.-X., Luo, C.-B., Song, H.-Q., & Lin, R.-Q. (2007). Identification of anisakid nematodes with zoonotic potential from Europe and China by single-strand conformation polymorphism analysis of nuclear ribosomal DNA. Parasitology Research, 101, 1703–1707.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Liang Li
    • 1
  • David I. Gibson
    • 2
  • Yuan-Yuan Liu
    • 1
  • Lu-Ping Zhang
    • 1
  1. 1.College of Life ScienceHebei Normal UniversityShijiazhuangPeople’s Republic of China
  2. 2.Department of ZoologyNatural History MuseumLondonUK

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