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A new entomopathogenic nematode, Steinernema robustispiculum n. sp. (Rhabditida: Steinernematidae), from Chumomray National Park in Vietnam

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Abstract

Steinernemar robustispiculum n. sp. (Rhabditida: Steinernematidae) was isolated from woodland in Chumomray National Park, Sason, Sathay, Kontum, Vietnam. Its morphology, morphometrics, cross-hybridisation and the ITS-rDNA sequence analysis revealed that S. robustispiculum clearly differs from other known Steinernema spp. As in the cases of S. intermedium (Poinar, 1985), S. robustispiculum has very robust spicules, but it can be distinguished by the longer tail of the infective juvenile, lower E%, shorter spicules, the shape of the spicules, the number of genital papillae in the caudal region and the presence of a mucron on the male tail. S. robustispiculum has a lateral field resembling that of S. sangi Phan, Nguyen & Moens, 2001, but can be distinguished by a higher E%, higher D%, smaller length to width ratio of the spicules and the morphology of both the spicule head (manubrium) and the dorsal lobe of the spicule. The morphometrics of infective juveniles of S. robustispiculum are similar to those of S. monticolum Stock, Choo & Kaya, 1997; these species can be distingusihed by the position of the excretory pore, the smaller length to width ratio of the spicules, and the length and morphology of the spicule head (manubrium). The phylogenetic relationships within Steinernema Travassos, 1927, including the newly sequenced Vietnamese species S. robustispiculum n. sp., S. loci Phan, Nguyen & Moens, 2001, S. thanhi Phan, Nguyen & Moens, 2001 and S. sangi, are presented based on analyses of the ITS-rDNA. The ITS RFLP profiles obtained from 17 different restriction enzymes are also presented.

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Authors and Affiliations

  1. Institute of Ecology and Biological Resources, National Centre for Science and Technology, Hoang Quoc Viet 18, Nghiado, Caugiay, Hanoi, Vietnam

    Long K. Phan

  2. Institute of Parasitology of Russian Academy of Sciences, Leninskii Prospect 33, Moscow, 117071, Russia

    Sergei A. Subbotin

  3. CLO-Department for Crop Protection, Burg. Van Gansberghelaan 96, Merelbeke, B 9820, Belgium

    Lieven Waeyenberge & Maurice Moens

  4. Laboratory for Agrozoology, Gent University, Coupure 555, Gent, 9000, Belgium

    Maurice Moens

Authors
  1. Long K. Phan
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  2. Sergei A. Subbotin
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  3. Lieven Waeyenberge
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  4. Maurice Moens
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Phan, L.K., Subbotin, S.A., Waeyenberge, L. et al. A new entomopathogenic nematode, Steinernema robustispiculum n. sp. (Rhabditida: Steinernematidae), from Chumomray National Park in Vietnam. Syst Parasitol 60, 23–32 (2005). https://doi.org/10.1007/s11230-004-1373-x

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  • DOI: https://doi.org/10.1007/s11230-004-1373-x

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