Exploring the role of the phage-specific insert of bacteriophage Φ11 dUTPase
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dUTPases are essential for maintaining genome integrity. Recently, in the case of a dUTPase from a Staphylococcal phage, another different physiological function was also suggested. Namely, it was shown that dUTPase from the Staphylococcus aureus bacteriophage Ф11 is capable of binding to the Staphylococcal Stl repressor protein. This binding interferes with the function of Stl. In the present study, we investigated the putative role of a phage dUTPase-specific peptide segment in the interaction of dUTPase with Stl and in impeding Stl–DNA complex formation. We show that dUTPase from Mycobacterium tuberculosis that lacks the phage-specific insert is also capable of disrupting the complexation between Stl and DNA. Hence, the insert segment is not essential for perturbation of the Stl function. However, we also demonstrate that in case of the phage dUTPase, the insert-lacking construct is deficient in perturbation of Stl activity. These findings clearly indicate that the phage-specific insert has a well-defined role only in the context of the phage dUTPase.
KeywordsdUTPase Non-canonical insert Protein–protein interaction Protein–DNA interaction Staphylococcal repressor
Authors thank for the support of Hungarian Scientific Research Fund OTKA [NK 84008, K109486]; Baross Program of the New Hungary Development Plan [3DSTRUCT, OMFB-00266/2010 REG-KM-09-1-2009-0050]; Hungarian Academy of Sciences ([TTK IF-28/2012]; MedinProt program); the ICGEB Research Grant to BGV; and the European Commission FP7 Biostruct-X project [Contract No. 283570]. Funding for open access charge: Hungarian Academy of Sciences.
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