Skip to main content

DNA interaction with synthetic polymers in solution

Structural Chemistry volume 18, pages 519–525 (2007)Cite this article

Abstract

DNA complexes with cationic polymers (polyvinylamine (PVA), polyallylamine (PAA), polydimethylaminoethylmethacrylate (PDMAEM), poly-(N,N,N-trimethylammonio)ethyl methacrylate chloride (PTMAEM), poly-l-lysine (PLL)) were investigated. It was shown that volume and persistent length of DNA do not change essentially at low cationic polymer concentration in a solution. DNA packaging in 0.005 M NaCl was observed at charge ratio N/P ≈ 1. Secondary DNA structure in complexes was not disrupted, and DNA was protected from protonation. The comparison between DNA packaging in complexes with polycations and DNA condensation induced by trivalent ions was made.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
 

References

  1. Lerman LS (1971) Proc Nat Acad Sci USA 68:1886

    Article  CAS  Google Scholar 

  2. Gosule LC, Schellman JA (1976) Nature 259:333

    Article  CAS  Google Scholar 

  3. Gosule LC, Schellman JA (1978) J Mol Biol 121:311

    Article  CAS  Google Scholar 

  4. Wilson RW, Bloomfield VA (1979) Biochemistry 18:2192

    Article  CAS  Google Scholar 

  5. Widom J, Baldwin RL (1980) J Mol Biol 144:431

    Article  CAS  Google Scholar 

  6. Olins DE, Olins AL (1971) J Mol Biol 57:437

    Article  CAS  Google Scholar 

  7. Marcuet R, Houssier C (1991) J Biomol Struct Dynam 9:159

    Google Scholar 

  8. Suwalski M, Traub W (1972) Biopolymers 11:2223

    Article  Google Scholar 

  9. Girod JC, Johnson WCJ, Hungtington SK, Maestre MF (1973) Biochemistry 12:5092

    Article  CAS  Google Scholar 

  10. Rau DC, Lee BK, Parsegian VA (1984) Proc Natl Acad Sci USA 81:2621

    Article  CAS  Google Scholar 

  11. Flory P (1953) Principles of polymer chemistry. Cornell UP, Ithaca NY

  12. Gerasimov OV, Boomer JA, Qualls MM, Thompson DH (1999) Adv Drug Delivery Rev 38:317

    Article  CAS  Google Scholar 

  13. Mel’nikova YS, Mel’nikov SM, Löfroth JE (1999) Biophys Chem 281:125

    Article  Google Scholar 

  14. Pouton CW, Lucas P, Thomas BJ, Uduehi AN, Milroy DA, Moss SH. (1998) J Control Release 53:289

    Article  CAS  Google Scholar 

  15. Smith AE (1995) Annu Rev Microbiol 49:807

    Article  CAS  Google Scholar 

  16. Kataoka K, Harada A, Naga Y (2001) Adv Drug Delivery Rev 47:113

    Article  CAS  Google Scholar 

  17. Jones NA, Hill IRC, Stolnik S, Bignotti F, Davis SS, Garnett MC (2000) Biochim Biophys Acta 1517:1

    CAS  Google Scholar 

  18. Cherng J-Y, Schuurmans-Nieuwenbroek NME, Jiskoot W, Talsma H, Zuidam NJ, Hennink WE, Crommelin DJA (1999) J Control Release 60:343

    Article  CAS  Google Scholar 

  19. Rungsardthong U, Deshpande M, Bailey L, Vamvakaki M, Armes SP, Garnett MC, Stolnik S (2001) J Control Release 73:359

    Article  CAS  Google Scholar 

  20. Kabanov AV (1999) Pharm Sci Technol Today 2:365

    Article  CAS  Google Scholar 

  21. Spack EG, Sorgi FL (2001) Drug Discov Today 6:186

    Article  CAS  Google Scholar 

  22. Howard KA, Dash PR, Read ML, Ward K, Tomkins LM, Nazarova O, Ulbrich K, Seymour LW. (2000) Biochim Biophys Acta 1475:245

    CAS  Google Scholar 

  23. Kiselev OI, Slita AV, Smirnova AV, Deeva EG, Grudinin MP, Panarin EF, Nazarova OV (1999) Dokl Akad Nauk 366:699

    CAS  Google Scholar 

  24. Bloomfield VA. (1997) Biopolymers 44:1471

    Article  Google Scholar 

  25. Deng H, Bloomfield VA (1999) Biophys J 77:1556

    Article  CAS  Google Scholar 

  26. Vasilevskaya VV, Khokhlov AR, Kidoaki S, Yoshikawa K (1997) Biopolymers 41:51

    Article  CAS  Google Scholar 

  27. Kasyanenko NA, Zanina AV, Nazarova OV, Panarin EF (1999) Langmuir 15:7912

    Article  CAS  Google Scholar 

  28. Kasyanenko NA, Zanina AV, Plotnikova LV, Simonenkov AA, Defrenne S, Nazarova OV, Panarin EF (1998) Macromol Symp 136:25

    CAS  Google Scholar 

  29. Harada S, Hasagawa S (1984) Makromol Chem, Rapid Commun 5:27

    Article  CAS  Google Scholar 

  30. Pavlov GM, Panarin EF, Korneeva EV, Kurochkin KV, Baikov VE, Ushakova VN (1990) Makromol Chem 191:2889

    Article  CAS  Google Scholar 

  31. Panarin EF, Tarasova NN, Gorbunova OP (1993) Russ J Appl Chem 66

  32. Stickler M (1984) Angew Makromol Chem 123/124:85

    CAS  Google Scholar 

  33. Pavlov GM, Korneeva EV, Ebel C, Gavrilova II, Nesterova N A, Panarin EF (2004) Polymer Sci, Ser A:46А

  34. Berne, BJ, Pecora, R (2000) Dynamic light scattering with applications to chemistry. biology, and physics. Dover

  35. Kasyanenko N, Abramchuk S, Blagodatskikh I, Bogdanov A, Gallyamov M, Kononov A, Kosmotynskaya Yu, Sitnikova N, Khokhlov A (2003) Polymer Sci, Ser A 45A:960

    Google Scholar 

  36. Provencher SW (1982) Comput Phys Commun 27:229

    Article  Google Scholar 

  37. Sibileva MA, Osipova TN, Zalenski AO, Chebyschan MA, Golikova AI, Vorobiev VI, Frisman EV (1976) Mol Biol (Russia) 10:514

    CAS  Google Scholar 

  38. Kasyanenko N, Arikainen N, Frisman E (1998) Biophys Chem 70:93

    Article  CAS  Google Scholar 

  39. Huey R, Mohr SC (1981) Biopolymers 20:2533

    Article  CAS  Google Scholar 

  40. Lerman, LS (1973) In: Dushesne J (ed) Physico-chemical properties of nucleic acids, vol 3, Academic Press, NY, p 59

  41. Kasyanenko NA, Bartoshevich SF, Frisman EV (1985) Mol Biol (Russia) 19:1386

    CAS  Google Scholar 

  42. Kasyanenko N, Prokhorova SA, Haya EEF, Sudakova SS, Frisman EV, Dyachenko SA, Smorygo NA, Ivin BA (1999) Colloids Surf A 148:121

    Article  CAS  Google Scholar 

  43. Kasyanenko N, Aia EEF, Bogdanov A, Kosmotynskaya Yu, Yakovlev K (2002) Mol Biol 36:594

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biophysics, Faculty of Physics, St. Petersburg State University, Uluanovskaya St., 1, Petrodvorets, St.-Petersburg, 198504, Russia

    Nina Kasyanenko, Daria Afanasieva, Boris Dribinsky & Dmitry Mukhin

  2. Institute of Macromolecular Compounds RAS, St. Petersburg, Russia

    Olga Nazarova & Evgeny Panarin

Authors
  1. Nina Kasyanenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Daria Afanasieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Boris Dribinsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dmitry Mukhin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Olga Nazarova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Evgeny Panarin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nina Kasyanenko.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kasyanenko, N., Afanasieva, D., Dribinsky, B. et al. DNA interaction with synthetic polymers in solution. Struct Chem 18, 519–525 (2007). https://doi.org/10.1007/s11224-007-9162-1

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11224-007-9162-1

Keywords

  • DNA-polymeric complexes
  • DNA condensation
  • Polyvinylamine
  • Polyallylamine
  • Polydimethylaminoethylmethacrylate
  • Polytrimethylammonioethyl methacrylate
  • Poly-l-lysine