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Identification of Bovine Leucocyte Adhesion Deficiency (BLAD) Carriers in Holstein and Brown Swiss AI Bulls in Iran

  • Animal Genetics
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Abstract

BLAD is a hereditary disease in Holstein dairy cattle. The defective allele of CD18 gene, which is responsible for this disease, has recessive inheritance. The recessive homozygous form (BL/BL) is lethal and since carrier animals have viability, BLAD frequency increases by use of carrier bulls in Artificial Insemination (AI). BLAD carriers can be detected easily by means of polymerase chain reaction followed by restriction analysis of the amplicons. In this study DNA samples from Holstein (n = 30) and Brown Swiss (n = 10) bulls from Abbas Abad AI center (Khorasan state of Iran) were analysed. A 101-bp fragment from the polymorphic region of CD18 gene located on chromosome 1 was amplified by PCR. Restriction enzymes TaqI and HaeIII were used to identify genotypes. Digestion products were screened by electrophoresis on 8% non-denaturing polyacrylamide gel and visualized by ethidium bromide staining. Frequencies of BL/TL (carrier) genotypes in Holstein and Brown Swiss bulls were 3.33% and 0%, respectively. Our pedigree studies of the carrier bull in this experiment revealed that the mutation was inherited by him from Hawkeye bull (CANM 369995, BL). Although the elimination of BLAD-carrier bulls from the Holstein world would be the most efficient method to control this genetic disorder, many BLAD-carrier bulls are still listed commercially for AI, and BLAD is still occurring in Iran. Monitoring the prevalence of BLAD carriers in random selected herds may be helpful in judging the effectiveness of the BLAD-control program.

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Authors and Affiliations

  1. Excellence Animal Science Center, College of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, Iran

    A. Norouzy, M. R. Nassiry, F. Eftekhari Shahrody & A. Javadmanesh

  2. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    M. R. Mohammad Abadi & G. E. Sulimova

  3. Department of Animal Science, Faculty of Agriculture, Tabriz University, Iran

    M. R. Mohammad Abadi

Authors
  1. A. Norouzy
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  2. M. R. Nassiry
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  3. F. Eftekhari Shahrody
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  4. A. Javadmanesh
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  5. M. R. Mohammad Abadi
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  6. G. E. Sulimova
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__________

From Genetika, Vol. 41, No. 12, 2005, pp. 1697–1701.

Original English Text Copyright © 2005 by Norouzy, Nassiry, Eftekhari Shahrody, Javadmanesh, Mohammad Abadi, Sulimova.

The text was submitted by the authors in English.

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Norouzy, A., Nassiry, M.R., Eftekhari Shahrody, F. et al. Identification of Bovine Leucocyte Adhesion Deficiency (BLAD) Carriers in Holstein and Brown Swiss AI Bulls in Iran. Russ J Genet 41, 1409–1413 (2005). https://doi.org/10.1007/s11177-006-0014-7

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  • DOI: https://doi.org/10.1007/s11177-006-0014-7

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