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Russian Journal of Genetics

, Volume 41, Issue 12, pp 1372–1380 | Cite as

Identification of LMW Glutenin-Like Genes from Secale sylvestre Host

  • H.-Y. Shang
  • Y.-M. Wei
  • H. Long
  • Z.-H. Yan
  • Y.-L. Zheng
Plant Genetics

Abstract

Three low-molecular-weight (LMW) glutenin-like genes (designated as Ssy1, Ssy2, and Ssy3) from Secale sylvestre Host were isolated and characterized. The three genes consist of a predicted highly conservative signal peptide with 20 amino acids, a short N-terminal region with 13 amino acids, a highly variable repetitive domain and a less variable C-terminal domain. The deduced amino acid sequences of the three genes were the LMW-m type due to a methionine residue at the N-terminus. The phylogenetic analysis indicated that the prolamin genes could be perfectly clustered into five groups, including HMW-GS, LMW-GS, α/β-, γ-, and κ-prolamin. The LMW glutenin-like genes of S. sylvestre were more orthologous with the LMW-GS genes of wheat and B hordein genes of barley, which also had been confirmed by the homology analysis with the LMW-GS of wheat at Glu-A3, Glu-B3, and Glu-D3 loci. These results indicated that a chromosome locus (designated as Glu-R3) might be located on the R genome of S. sylvestre with the functions similar to the Glu-3 locus in wheat and its related species.

Keywords

Peptide Methionine Signal Peptide Deduce Amino Acid Sequence Chromosome Locus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© MAIK "Nauka/Interperiodica" 2005

Authors and Affiliations

  • H.-Y. Shang
    • 1
  • Y.-M. Wei
    • 1
  • H. Long
    • 1
  • Z.-H. Yan
    • 1
  • Y.-L. Zheng
    • 1
  1. 1.Triticeae Research InstituteSichuan Agricultural UniversityDujiangyan, SichuanChina

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