Abstract
The cytoplasm status according to mitochondrial sequence tags was determined in agamospermous sugar beet progenies characterized by unstable manifestation of cytoplasmic male sterility. The detected variations in the ratios of homologous sequences related to the N and S mtDNA types did not correlate with pollen phenotypes of the plants. Polymerase chain reaction allowed semiquantitative evaluation of these variations and their detection. A cDNA corresponding to a hitherto unknown minor RNA and containing a consensus sequence of dicotyledonous plant promoters was detected by mtRNA display. Probable sources of CMS variability in agamospermous sugar beet progenies are discussed.
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REFERENCES
Owen, F.V., Cytoplasmically Inherited Male Sterility in Sugar Beet, J. Agric. Res., 1945, vol. 71, pp. 423–440.
Maletskaya, E.I., Yudanova, S.S., and Maletskii, S.I., Expression of CMS in Zytogic and Apozygotic Progenies of Sugar Beet Beta vulgaris L., Rus. J. Genet., 2002, vol. 38, no.5, pp. 531–537.
Kubo, T., Nishizawa, S., and Mikami, T., Alteration in Organization and Transcription of the Mitochondrial Genome of Cytoplasmic Male Sterile Sugar Beet (Beta vulgaris L.), Mol. Gen. Genet., 1999, vol. 262, pp. 283–290.
Xue, Y., Collin, S., Davies, D.R., and Thomas, C.M., Differential Screening of Mitochondrial cDNA Libraries from Male-Sterile and Male-Fertile Sugar Beet Reveals Genome Rearrangements at atpA and atp6 Loci, Plant. Mol. Biol., 1994, vol. 25, pp. 91–103.
Satoh, M., Kubo, T., Nishizawa, S., et al., The Cytoplasmic Male-Sterile Type and Normal Type Mitochondrial Genomes of Sugar Beet Share the Same Complement of Genes of Known Function but Differ in the Content of Expressed ORFs, Mol. Gen. Genomics, 2004, vol. 272, pp. 247–256.
Khvorostov, I.B., Veprev, S.G., Maletskii, S.I., and Dymshits, G.M., PCR Analysis of Cytoplasm Conversion from the N to the S Type in Sugar Beet (Beta vulgaris L.), Dokl. Akad. Nauk, 1997, vol. 357, no.4, pp. 572–574.
Maletskii, S.I., Khvorostov, I.B., Maletskaya, E.I., et al., The Variability of Pollen Grains Sterility-Fertility Character in Sugar Beet Plants with S-Type of Cytoplasm for Agamospermic Mode of Seed Reproduction, Sugar Tech., 1999, vol. 1, no.4, pp. 139–145.
Veprev, S.G., Khvorostov, I.B., and Dymshits, G.M., Variation of the Cytoplasm Type in Sugar Beet (Beta vulgaris L.) upon Inbreeding: Influence of Hybridization, Rus. J. Genet., 2003, vol. 39, no.6, pp. 661–668.
Rogers, S.O. and Bendich, A.J., Extraction of DNA from Milligram Amounts of Fresh, Herbarium and Mummified Plant Tissues, Plant. Mol. Biol., 1985, vol. 5, pp. 69–76.
Hilary, J.R., Nigel, A.B., and Parkes, H.C., Comparison of Small-Scale Methods for the Rapid Extraction of Plant DNA Suitable for PCR Analysis, Plant. Mol. Biol. Rep., 1996, vol. 14, pp. 170–183.
Dudareva, N.A., Popovsky, A.V., Kasjanova, U.V., et al., Expression of Mitochondrial Genes in Fertile and Sterile Sugar Beet Cytoplasms with Different Nuclear Fertility Restorer Genes, Theor. Appl. Genet., 1991, vol. 83, pp. 217–224.
Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1989.
Ivanov, M.K., Revenko, A.S., and Dymshits, G.M., Structural Variation of the Mitochondrial Genome in Sugar Beet (Beta vulgaris L.) in the rps3 and orf215 Gene Regions Associated with Cytoplasmic Male Sterility, Mol. Biol. (Moscow), 2004, vol. 38, no.3, pp. 413–419.
Ivanov, M.K., Revenko, A.S., Kabilov, M.R., and Dymshits, G.M., RARD Analysis of Sugar Beet Mitochondrial DNA: Use to Search for Genes of the CMS System, Dokl. Akad. Nauk, 2002, vol. 387, no.2, pp. 279–281.
Nemchinov, L.G., Hammond, J., Jordan, R., and Hammond, R.W., The Complete Nucleotide Sequence, Genome Organization, and Specific Detection of Beet Mosaic Virus, Arch. Virol., 2004, vol. 49, no.6, pp. 1201–1214.
Veprev, S.G., Dudareva, N.A., Maletskii, S.I., and Mglinets, A.V., Female Digamy Genetic Systems Controlling Pollen Sterility, in Geneticheskii kontrol' razmnozheniya sakharnoi svekly (Genetic Control of Reproduction in Sugar Beet), Novosibirsk: Nauka, 1991, pp. 114–157.
Ducos, E., Touzet, P., Saumitou-Laprade, P., et al., Nuclear Effect on Mitochondrial Protein Expression of the CMS Owen Cytoplasm in Sugar Beet, Theor. Appl. Genet., 2001, vol. 102, pp. 1299–1304.
Yui, R., Iketani, S., Mikami, T., and Kubo, T., Antisense Inhibition of Mitochondrial Pyruvate Dehydrogenase E1 Subunit in Anther Tapetum Causes Male Sterility, Plant J., 2003, vol. 34, pp. 57–66.
Hjerdin-Panagopoulos, A., Kraft, T., Rading, I.M., et al., Three QTL Regions for Restoration of Owen CMS in Sugar Beet, Crop Sci., 2004, vol. 42, pp. 540–544.
Hattori, N., Kitagawa, K., Takumi, S., and Nakamura, C., Mitochondrial DNA Heteroplasmy in Wheat, Aegilops and Their Nucleus-Cytoplasm Hybrids, Genetics, 2002, vol. 160, pp. 1619–1630.
Laser, B., Mohr, S., Odenbach, W., Oettler, G., and Kuck, U., Paternal and Novel Copies of the Mitochondrial orf25 Gene in the Hybrid Crop-Plant Triticale: Predominant Transcriptional Expression of the Maternal Gene Copy, Curr. Genet., 1997, vol. 32, pp. 337–347.
Aul'chenko, Yu.S., Veprev, S.G., and Aksenovich, T.I., Change of the Cytoplasm Type in Sugar Beet (Beta vulgaris L.) during Inbreeding: Segregation Analysis of a Pedigree, Rus. J. Genet., 1997, vol. 33, no.7, pp. 797–802.
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Translated from Genetika, Vol. 41, No. 11, 2005, pp. 1511–1521.
Original Russian Text Copyright © 2005 by Ivanov, Revenko, Maletskaya, Maletskii, Dymshits.
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Ivanov, M.K., Revenko, A.S., Maletskaya, E.I. et al. Structural and Transcriptional Variation of Mitochondrial DNA in Pollen-Sterile Agamospermous Sugar Beet (Beta vulgaris L.) Progeny. Russ J Genet 41, 1245–1253 (2005). https://doi.org/10.1007/s11177-005-0225-3
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DOI: https://doi.org/10.1007/s11177-005-0225-3