Abstract
Polymorphisms of the prolactin (bPRL) and growth hormone (bGH) genes were studied comparatively in the Russian and German Black-and-White and Yaroslavl cattle breeds. Two polymorphisms were studied for each gene. In the case of the bPRL gene, the polymorphism of the 5′-untranslated region was examined by microsatellite analysis and the RsaI polymorphism of exon 3, by RFLP analysis. In the case of the bGH gene, the MspI polymorphism of intron III and the AluI polymorphism of exon 5 were assessed by RFLP analysis. Differences in allele and genotype frequencies were observed both between and within breeds. The heterozygosity at the RsaI marker was low (9.4%) in the Russian Black-and-White breed; that at the microsatellite of the bPRL gene was low (3.2–24%) in all breeds examined. Homozygotes BB at the bPRL gene, which had not been reported earlier for European cattle breeds, were detected in the German Black-and-White and Yaroslavl breeds (at frequencies 0.16 and 0.13, respectively). The frequency of allele MspI(−) of the bGH gene in the Yaroslavl breed was extremely low (0.02), comparable only with that of the Holstein cattle (0.02). The heterozygosity at the AluI polymorphism was higher than at the MspI polymorphism of the bGH gene and reached 55% in the Yaroslavl breed. Genotype BB of the RsaI polymorphism of the bPRL gene tended to show a negative association with the fat content in milk. The genotypes of the AluI polymorphism of the bGH gene were associated with the fat content in milk in the Yaroslavl (F=4.56, P=0.013) and German Black-and-White (F=4.1, P=0.041) breeds: the highest fat content in milk was observed in the subsample of cows with heterozygous genotype VL.
Similar content being viewed by others
REFERENCES
Akers, R.M., Bauman, D.E., Capuco, A.V., et al., Prolactin Regulation of Milk Secretion and Biochemical Differentiation of Mammary Epithelial Cells in Periparturient Cows, Endocrinology, 1981, vol. 109, pp. 23–30.
Mitra, A., Schlee, P., Balakrishnan, C.R., et al., Polymorphism at Growth Hormone and Prolactin Loci in Indian Cattle and Buffalo, J. Anim. Breed. Genet., 1995, vol. 112, pp. 71–74.
Chrenek, P., Vasicek, D., Bauerovf, M., et al., Simultaneous Analysis of Bovine Growth Hormone and Prolactin Alleles by Multiplex PCR and RFLP, Czech. J. Anim. Sci., 1998, vol. 43, pp. 53–55.
MacHugh, D.E., Shriver, M.D., Loftus, R.T., et al., Microsatellite DNA Variation and the Evolution, Domestication and Phylogeography of Taurine and Zebu Cattle (Bos taurus and Bos indicus), Genetics, 1997, vol. 146, pp. 1071–1086.
Hoj, S., Fredholm, M., Larsen, N.J., et al., Growth Hormone Gene Polymorphism Associated with Selection for Milk Fat Production in Lines of Cattle, Anim. Genet., 1993, vol. 24, pp. 91–96.
Lagziel, A., Lipkin, E., Ezra, E., et al., An MspI Polymorphism at the Bovine Growth Hormone (bGH) Gene Is Linked to a Locus Affecting Milk Protein Percentage, Anim. Genet., 1999, vol. 30, pp. 296–299.
Lagziel, A. and Soller, M., DNA Sequence of SSCP Haplotypes at the Bovine Growth Hormone (bGH) Gene, Anim. Genet., 1999, vol. 30, pp. 362–365.
Yao, J., Aggrey, S.E., Zadworny, D., et al., Sequence Variations in the Bovine Growth Hormone Gene Characterized by Single-Strand Conformation Polymorphism (SSCP) Analysis and Their Association with Milk Production Traits in Holsteins, Genetics, 1996, vol. 144, pp. 1809–1816.
Sabour, M.P., Lin, C.Y., Lee, A.J., et al., Association between Milk Protein Genetic Variants and Genetic Values of Canadian Holstein Bulls for Milk Yield Traits, J.Dairy Sci., 1996, vol. 79, no.6, pp. 1050–1056.
Lucy, M.C., Hauser, S.D., Eppard, P.J., et al., Variants of Somatotropin in Cattle: Gene Frequencies in Major Dairy Breeds and Associated Milk Production, Domest. Anim. Endocrinol., 1993, vol. 10, pp. 325–333.
Lee, B.K., Lin, G.F., Crocker, B.A., et al., Association of Somatotropin (bST) Gene Polymorphism with Selection for Milk Yield in Holstein Cows, J. Dairy Sci., 1993, vol. 76,Suppl. 1, p. 149.
Lee, B.K., Lin, G.F., Crocker, B.A., et al., Association of Somatotropin (bST) Gene Polymorphism at the 5th Exon with Selection for Milk Yield in Holstein Cows, Domest. Anim. Endocrinol., 1996, vol. 13, pp. 373–381.
Grochowska, R., Zwierzchowski, L., Snochowski, M., et al., Stimulated Growth Hormone (GH) Release in Friesian Cattle with Respect to GH Genotypes, Reprod. Nutr. Dev., 1999, vol. 39, no.2, pp. 171–180.
Udina, I.G., Turkova, S.O., and Kostyuchenko, M.V., et al. Polymorphism of Bovine Prolactin Gene: Microsatellites, PCR-RFLP, Rus. J. Genet., 2001, vol. 37, no.4, pp. 407–411.
Altukhov, Yu. P., Geneticheskie protsessy v populyatsiyakh (Genetic Processes in Populations), Moscow: Nauka, 1989.
Rokitskii, P.F., Osnovy variatsionnoi statistiki dlya biologov (Elements of Variation Statistics for Biologists), Minsk: Bel. Gos. Univ., 1961.
Ayala, F., Vvedenie v populyatsionnuyu genetiku (Introduction to Population Genetics), Moscow: Mir, 1984.
Yeh, F.C., Yang, R.-C., and Boyle, T., POPGENE VERSION 1.31: Microsoft Window-Based Freeware for Population Genetic Analysis Quick User Guide, 1999.
StatSoft, STATISTICA for Windows (Computer Program Manual), Tulsa, OK: StatSoft, 1996.
Lewin, H.A., Schmitt, K., Hubert, R., et al., Close Linkage between Bovine Prolactin and BoLA-DRB3 Genes Mapping in Cattle by Single Sperm Typing, Genomics, 1992, vol. 13, pp. 44–48.
Lagziel, A., DeNise, S., Hanotte, O., et al., Geographic and Breed Distribution of an MspI PCR-RFLP in the Bovine Growth Hormone (bGH) Gene, Anim. Genet., 2000, vol. 31, pp. 210–213.
Author information
Authors and Affiliations
Additional information
Translated from Genetika, Vol. 41, No. 2, 2005, pp. 229–236.
Original Russian Text Copyright © 2005 by Khatami, Lazebny, Maksimenko, Sulimova.
Rights and permissions
About this article
Cite this article
Khatami, S.R., Lazebny, O.E., Maksimenko, V.F. et al. Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and Black-and-White cattle. Russ J Genet 41, 167–173 (2005). https://doi.org/10.1007/s11177-005-0040-x
Received:
Issue Date:
DOI: https://doi.org/10.1007/s11177-005-0040-x