Abstract
Mutants capable of a high frequency of cytoduction (Hfc+) were obtained in a haploid strain of Saccharomyces cerevisiae, suggesting impaired cytogamy. Nine of the 68 Hfc+ mutants showed the antisuppressor effect with respect to mutations of the SUP35 and SUP45 genes, which code for translation termination factors, or to the [PSI +] factor, which is the prion form of Sup35. Cosegregation of the characters “higher frequency of cytoduction” and “antisuppression” was demonstrated for three Hfc+ mutants. One (HFC12-2) of the Hfc+ mutations exerted a dominant antisuppressor effect with respect to [PSI +] and had no effect on [PSI +] maintenance. On the strength of the results, an interaction was assumed for translation termination components and cytoskeleton proteins, which play a role in karyogamy in yeasts.
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Translated from Genetika, Vol. 41, No. 2, 2005, pp. 178–186.
Original Russian Text Copyright © 2005 by Borchsenius, Repnevskaya, Kurischko, Inge-Vechtomov.
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Borchsenius, A.S., Repnevskaya, M.V., Kurischko, C. et al. Association between defects of karyogamy and translation termination in yeast Saccharomyces cerevisiae . Russ J Genet 41, 122–129 (2005). https://doi.org/10.1007/s11177-005-0035-7
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DOI: https://doi.org/10.1007/s11177-005-0035-7