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Effect of the dinitrosyl iron complex with N-ethylthiourea on ROS and NO intracellular levels and caspase activity in HeLa tumor cells

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Abstract

The kinetics of intracellular accumulation of nitrogen monoxide and reactive oxygen species under the action of various concentrations of the dinitrosyl iron complex with N-ethylthiourea was described for the first time in in vitro experiments. Both a significant accumulation of NO by HeLa tumor cells and a substantial increase in the generation of intracellular reactive oxygen species under the action of the complex were shown. A decrease in the viability of tumor cells was revealed accompanied by an increase in the activity of proapoptotic enzymes of caspase 3 and caspase 8 after 6 h of the complex action. The data obtained suggest an important role of reactive oxygen species and reactive nitrogen species in the cytotoxic effect of the dinitrosyl iron complex with N-ethylthiourea on the tumor cells.

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Authors and Affiliations

  1. Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry, Russian Academy of Sciences, 1 prosp. Akad. Semenova, 142432, Chernogolovka, Moscow Region, Russian Federation

    V. I. Amozova, A. A. Balakina, D. V. Mishchenko & N. A. Sanina

  2. Scientific and Educational Center in Chernogolovka, Moscow Region State Pedagogical University, 24 ul. Very Voloshinoi, 141014, Mytishchi, Moscow Region, Russian Federation

    D. V. Mishchenko & N. A. Sanina

  3. Faculty of Fundamental Physical and Chemical Engineering, Lomonosov Moscow State University, 1 Leninskie Gory, 119991, Moscow, Russian Federation

    D. V. Mishchenko & N. A. Sanina

Authors
  1. V. I. Amozova
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  2. A. A. Balakina
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  3. D. V. Mishchenko
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  4. N. A. Sanina
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Corresponding author

Correspondence to A. A. Balakina.

Additional information

Dedicated to Academician of the Russian Academy of Sciences I. P. Beletskaya on the occasion of her anniversary.

This work was financially supported by the Ministry of Science and Higher Education of the Russian Federation (Projects Nos AAAA-A19-119092390041-5 and AAAA-A19-119071890015-6) and the Russian Foundation for Basic Research (Project No. 20-03-00183A).

No human or animal subjects were used in this research.

The authors declare no competing interests.

Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, Vol. 72, No. 4, pp. 1066–1074, April, 2023.

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Amozova, V.I., Balakina, A.A., Mishchenko, D.V. et al. Effect of the dinitrosyl iron complex with N-ethylthiourea on ROS and NO intracellular levels and caspase activity in HeLa tumor cells. Russ Chem Bull 72, 1066–1074 (2023). https://doi.org/10.1007/s11172-023-3872-9

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  • DOI: https://doi.org/10.1007/s11172-023-3872-9

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