Abstract
A novel method of high-performance flash chromatography was proposed. The method allows one to separate mixtures of substances ranging from hundreds of milligrams to several grams with close chromatographic mobility (up to ΔRf 0.05). An enhanced efficiency of chromatographic columns is achieved by using fine-grained silica gel (5–40 µm), special packing of the column, and a procedure of supporting the mixture to be separated onto the adsorbent layer, which ensures the formation of a narrow zone of the substances on the adsorbent layer at the beginning of elution.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
W. C. Still, M. Kahn, A. Mitra, J. Org. Chem., 1978, 43, 2923–2925; DOI: https://doi.org/10.1021/jo00408a041.
B. Roge, S. N. Firke, R. M. Kawade, S. K. Sarje, S. M. Vadvalkar, IJPSR, 2011, 2, 1930–1937.
H. Chaudhari, F. Chaudhari, M. Patel, P. K. Pradhan, U. M. Upadhyay, IJPDT, 2012, 2, 80–84.
D. Girija, A. Padma, B. V. Reddy, IJCPS, 2018, 6, 300–305.
A. Kasprowiak, F Cazier-Dennin, P.-E. Danjou, J. Chem. Educ., 2020, 97, 1145–1150; DOI: https://doi.org/10.1021/acs.jchemed.9b00929.
W. C. Stevens, Jr., D. C. Hill, Mol. Divers., 2009, 13, 247–252; DOI: https://doi.org/10.1007/s11030-008-9104-x.
A. J. Shusterman, P. G. McDougal, A. Glasfeld, J. Chem. Ed., 1997, 74, 1222–1223; DOI: https://doi.org/10.1021/ed300544n.
D. S. Pedersen, C. Rosenbohm, Synthesis, 2001, 2431–2434; DOI: https://doi.org/10.1055/s-2001-18722.
N. M. Targett, J. P. Kilcoyne, B. Green, J. Org. Chem., 1979, 44, 4962–4966; DOI: https://doi.org/10.1021/jo00394a045.
L. R. Snyder, J. Chromatogr. Sci., 1977, 15, 441–449; DOI: https://doi.org/10.1093/chromsci/15.10.441.
R. P. Bokaldere, A. Ya. Liepinya, E. V. Lozha, D. O. Lolya, Ya. F. Freimanis, J. Org. Chem. USSR, 1981, 17, 2119–2123.
M. A. Lapitskaya, T. A. Manukina, A. G. Nozdracheva, L. G. Sheimina, K. K. Pivnitskii, J. Org. Chem. USSR, 1983, 19, 261–265; https://doi.org/10.1002/chin.198328369.
Author information
Authors and Affiliations
Corresponding author
Additional information
No human or animal subjects were used in this research.
The authors declare no competing interests.
† Deceased.
TPN = 5.55r/d, where r is the distance from the chromatogram onset to the top of the substance peak, and d is the width of the peak of the substance at half of its height.10
Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 6, pp. 1224–1230, June, 2022.
Rights and permissions
About this article
Cite this article
Pivnitskiy, K.K., Vasiljeva, L.L. & Veselovsky, V.V. High-performance flash chromatography on fine-grained silica gel. Russ Chem Bull 71, 1224–1230 (2022). https://doi.org/10.1007/s11172-022-3523-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11172-022-3523-1