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NMR signal assignments afnd secondary structure determination of the N-terminal domain of the ribosome maturation factor M from Staphylococcus aureus

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Abstract

The ribosome maturation factor M (RimM) is a protein with a molecular mass of 19.072 kDa involved in assembling of the 30S ribosome subunit. The RimM is necessary for the efficient processing of 16S rRNA. However, the mechanism of interaction of the RimM N-terminal domain with 16S rRNA remains poorly studied. The synthesis of the N-terminal domain of RimM from Staphylococcus aureus enriched in 13C and 15N isotopes and subsequent analysis of chemical shifts of the 1H, 13C, and 15N signals from the backbone and side chains are described. An analysis of chemical shifts suggests that the N-terminal domain of RimM contains six β-chains and three α-helices with the topology β1-β2-α1-β3-β4-β5-α2-β6-β3. The secondary structure of the N-terminal domain of RimM contains a KH domain between the β1 and β2 fold with a strongly conserved segment with the GXXG sequence. The further structural studies by integrated structural biology approach (NMR spectroscopy, X-ray diffraction analysis, and cryoelectron microscopy) of RimM and its complex with ribosome will allow screening of highly selective inhibitors of Staphylococcus aureus translation.

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Funding

This work was financially supported by the Russian Foundation for Basic Research (Project No. 20-34-70021).

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Authors and Affiliations

  1. Kazan (Near-Volga) Federal University, 18 ul. Kremlevskaya, 420008, Kazan, Russia

    N. S. Garaeva, A. G. Bikmullin, E. S. Kuchaev, E. A. Klochkova, Sh. Z. Validov, V. V. Klochkov, A. V. Aganov & K. S. Usachev

  2. Federal Research Center “Kazan Scientific Center of Russian Academy of Sciences”, 2/31 ul. Lobachevskogo, 420008, Kazan, Russia

    E. S. Kuchaev, Sh. Z. Validov, M. M. Yusupov & K. S. Usachev

  3. Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS UMR7104, INSERM U964, Université de Strasbourg, 1 rue Laurent Fries, F-67400, Illkirch, France

    M. M. Yusupov

Authors
  1. N. S. Garaeva
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  2. A. G. Bikmullin
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  3. E. S. Kuchaev
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  4. E. A. Klochkova
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  5. Sh. Z. Validov
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  6. V. V. Klochkov
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  7. A. V. Aganov
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  8. M. M. Yusupov
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  9. K. S. Usachev
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Corresponding authors

Correspondence to E. S. Kuchaev, M. M. Yusupov or K. S. Usachev.

Additional information

Dedicated to Academician of the Russian Academy of Sciences R. Z. Sagdeev on the occasion of his 80th birthday.

Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 12, pp. 2440–2444, December, 2021.

This paper does not contain descriptions of studies on animals or humans.

The authors declare no competing interests.

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Garaeva, N.S., Bikmullin, A.G., Kuchaev, E.S. et al. NMR signal assignments afnd secondary structure determination of the N-terminal domain of the ribosome maturation factor M from Staphylococcus aureus. Russ Chem Bull 70, 2440–2444 (2021). https://doi.org/10.1007/s11172-021-3365-2

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  • DOI: https://doi.org/10.1007/s11172-021-3365-2

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