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Model bacterial system to study the possible effect of strong magnetic fields on biochemical reactions involving free radicals at the cellular level

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Abstract

The possibility of adapting a chemical system for generating nitric oxide (NO) and superoxide anion (O2•−) upon the decomposition of 3-morpholinosydnonimine (SIN-1) in a buffer solution to the conditions of a similar experiment on bacterial cultures in the field of an MRI scanner was examined. For this system, the magnetic field effect on the recombination of the two radicals to form peroxynitrite (ONOO−) in a range of strong magnetic fields was experimentally found earlier and explained by the Δg mechanism. The transition from a model in vitro system involving biologically relevant species NO, O2•−, and ONOO− to an in vivo system and the development of a reliable model system with a reproducible magnetic field effect that can be employed as a working tool in further studies were considered. The decomposition of SIN-1 in the presence of components of an LB culture medium for bacterial cultures and the redox activity of the medium itself and a possible autoinitiation of the Fenton reaction on the intrinsic iron content were studied by spectrophotometry and ESR spectroscopy of spin traps. A decrease in the viability and formation of filaments for the E.coli JM109 strain were observed in experiments on the exposition of the bacterial strains in the presence of radical donors in a strong magnetic field of 11.7 T.

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Funding

The authors are grateful to the Center for Collective Use of Microscopic Analysis of Biological Objects of the Siberian Branch of the Russian Academy of Sciences and to the Center for Collective Use “SPF-Vivarium” of the Institute of Cytology and Genetics (Siberian Branch of the Russian Academy of Sciences).

This work was financially supported by the Ministry of Education and Science (Project No. 0259-2021-0010 of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences).

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 10 prosp. Akad. Lavrent’eva, 630090, Novosibirsk, Russia

    S. V. Sergeeva & D. V. Petrovsky

  2. V. V. Voevodsky Institute of Chemical Kinetics and Combustion, Siberian Branch of the Russian Academy of Sciences, 3 ul. Institutskaya, 630090, Novosibirsk, Russia

    T. V. Kobzeva & D. V. Stass

Authors
  1. S. V. Sergeeva
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  2. D. V. Petrovsky
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  3. T. V. Kobzeva
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  4. D. V. Stass
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Corresponding author

Correspondence to D. V. Stass.

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Dedicated to Academician of the Russian Academy of Sciences R. Z. Sagdeev on the occasion of his 80th birthday.

Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 12, pp. 2332–2338, December, 2021.

This paper does not contain descriptions of studies on animals or humans.

The authors declare no competing interests.

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Sergeeva, S.V., Petrovsky, D.V., Kobzeva, T.V. et al. Model bacterial system to study the possible effect of strong magnetic fields on biochemical reactions involving free radicals at the cellular level. Russ Chem Bull 70, 2332–2338 (2021). https://doi.org/10.1007/s11172-021-3349-2

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  • DOI: https://doi.org/10.1007/s11172-021-3349-2

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