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Enzymatic hydrolysis of collagen by pancreatin and thrombin as a step in the formation of scaffolds

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Abstract

High-molecular collagen was isolated from cod skin and subjected to enzymatic hydrolysis with pancreatin and thrombin. The resulting products were characterized by molecular weight parameters. It is shown that formation of two oligomeric fractions with molecular masses of 9 kDa (>80%) and 17–18 kDa (2–4%) is characteristic of both the pancreatin and thrombin hydrolysis of the peptide bonds in collagen.

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Authors and Affiliations

  1. Lobachevsky State University of Nizhni Novgorod — National Research University, 23 prosp. Gagarina, 603950, Nizhny Novgorod, Russian Federation

    L. L. Semenycheva, V. O. Chasova, N. B. Valetova, M. V. Podguzkova & Yu. L. Kuznetsova

  2. Privolzhsky Research Medical University of the Ministry of Health of the Russian Federation, 10/1 Minin and Pozharsky pl., 603950, Nizhny Novgorod, Russian Federation

    M. N. Egorikhina

  3. Helixan Ltd., 74 ul. Generala Lizyukova, 394088, Voronezh, Russian Federation

    M. V. Astanina

Authors
  1. L. L. Semenycheva
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  2. M. N. Egorikhina
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  3. V. O. Chasova
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  4. N. B. Valetova
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  5. M. V. Podguzkova
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  6. M. V. Astanina
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  7. Yu. L. Kuznetsova
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Correspondence to L. L. Semenycheva.

Additional information

Based on the materials of the V International Scientific Conference “Advances in Synthesis and Complexing” (April 22–26, 2019, Moscow, Russia).

Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 1, pp. 0164–0168, January, 2020.

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Semenycheva, L.L., Egorikhina, M.N., Chasova, V.O. et al. Enzymatic hydrolysis of collagen by pancreatin and thrombin as a step in the formation of scaffolds. Russ Chem Bull 69, 164–168 (2020). https://doi.org/10.1007/s11172-020-2738-2

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  • DOI: https://doi.org/10.1007/s11172-020-2738-2

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