Lipase enzymatic microreactor in polysaccharide hydrogel: structure and properties

Abstract

An enzymatic microreactor was designed on the basis of sodium alginate hydrogel containing a lipolytic enzyme (Candida rugosa lipase) in the polymer matrix pores. The functional properties of lipase were characterized in polysaccharide hydrogel of different concentrations. The polysaccharide hydrogel matrix was shown to act as a microreactor, where the enzyme retains activity during numerous catalytic cycles and allows designing long-acting drug systems of enzymatic nature.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    P. Woolley, S. B. Petersen, Lipases–Their Structure, Biochemistry and Applications, Cambridge University Press, Cambridge, 2012, 382 pp.

    Google Scholar 

  2. 2.

    V. R. Murty, J. Bhat, P. K. A. Muniswaran, Biotechnol. Bioprocess Eng., 2002, 7, 57.

    CAS  Article  Google Scholar 

  3. 3.

    M. A. Pernas, L. Pastrana, P. Fucinos, M. L. Rua, J. Phys. Org. Chem., 2009, 22, 508.

    CAS  Article  Google Scholar 

  4. 4.

    G. Fernandez-Lorente, Z. Cabrera, C. Godoy, R. Fernandez-Lafuente, J. M. Palomo, G. M. Guisan, Process Biochem., 2008, 43, 1061.

    CAS  Article  Google Scholar 

  5. 5.

    P. Reis, K. Holmberg, H. Watzke, M. E. Leser, R. Miller, Adv. Colloid Interface Sci., 2009, 147–148, 237.

    Article  Google Scholar 

  6. 6.

    R. Verger, G. H. de Haas, Annu. Rev. Biophys. Bioeng., 1976, 5, 77.

    CAS  Article  Google Scholar 

  7. 7.

    T. Thuren, FEBS Letters, 1988, 229, 95.

    CAS  Article  Google Scholar 

  8. 8.

    Y. Cajal, A. Svendsen, J. De Bolos, S. A. Patkar, M. A. Alsina, Biochimie, 2000, 82, 1053.

    CAS  Article  Google Scholar 

  9. 9.

    L. R. Bogdanova, E. A. Ermakova, B. Z. Idiyatullin, L. Ya. Zakharova, A. I. Konovalov, Yu. F. Zuev, Dokl. Biochem. Biophys., 2012, 446, 238.

    CAS  Article  Google Scholar 

  10. 10.

    L. H. Yahia, N. Chirani, L. Gritsch, F. L. Motta, S. Chirani, S. Faré, J. Biomed. Sci., 2015, 4, No. 2, 1.

    Article  Google Scholar 

  11. 11.

    S. R. Derkach, N. G. Voron´ko, Yu. A. Kuchina, D. S. Kolotova, A. M. Gordeeva, D. A. Faizullin, Yu. A. Gusev, Yu. F. Zuev, O. N. Makshakova, Carbohydr. Polym., 2018, 197, 66.

    CAS  Article  Google Scholar 

  12. 12.

    J. Radhakrishnan, A. Subramanian, U. M. Krishnan, S. Sethur aman, Biomacromolecules, 2017, 18, 1.

    CAS  Article  Google Scholar 

  13. 13.

    N. Eslahi, M. Abdorahim, A. Simchi, Biomacromolecules, 2016, 17, 3441.

    CAS  Article  Google Scholar 

  14. 14.

    C. Alvarez-Lorenzo, B. Blanco-Fernandez, A. M. Puga, A. Concheiro, Adv. Drug Deliv. Rev., 2013, 65, 1148.

    CAS  Article  Google Scholar 

  15. 15.

    R. Pogaku, K. R. Jegannathan, Production of Biodiesel Using Lipase Incapsulated in k-Carrageenan, Springer Briefs in Bioengineering, 2015, 125 pp.

    Google Scholar 

  16. 16.

    J. P. Paques, E. van der Linden, C. J. van Rijn, L. M. Sagis, Adv. Colloid Interface Sci., 2014, 209, 163.

    CAS  Article  Google Scholar 

  17. 17.

    C.-S. Wu, C.-T. Wu, Y.-S. Yang, Fu.-H. Ko, Chem. Commun., 2008, 42, 5327.

    Article  Google Scholar 

  18. 18.

    E. A. Burstein, S. M. Abornev, Ya. K. Reshetnyak, Biophys. J., 2001, 81, 1699.

    CAS  Article  Google Scholar 

  19. 19.

    A. A. Mendez, B. C. M. Barbosa, M. L. C. P. Da Silva, H. F. De Castro, Biocatal. Biotransfor., 2007, 25, 393.

    Article  Google Scholar 

  20. 20.

    G. E. A. Awad, H. R. Wehaidy, A. A. Abd El Aty, M. E. Hassan, Colloid Polym. Sci., 2017, 295, 495.

    CAS  Article  Google Scholar 

  21. 21.

    L. R. Bogdanova, D. R. Bakirova, Y. A. Valiullina, B. Z. Idiyatullin, D. A. Faizullin, Y. F. Zuev, O. S. Zueva, Biophysics, 2016, 61

    Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Yu. F. Zuev.

Additional information

Dedicated to Academician of the Russian Academy of Sciences A. I. Konovalov on the occasion of his 85th birthday.

Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 2, pp. 0400–0404, February, 2019.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Bogdanova, L.R., Rogov, A.M., Zueva, O.S. et al. Lipase enzymatic microreactor in polysaccharide hydrogel: structure and properties. Russ Chem Bull 68, 400–404 (2019). https://doi.org/10.1007/s11172-019-2399-1

Download citation

Key words

  • polysaccharide hydrogel
  • lipase
  • immobilization
  • enzymatic catalysis