Abstract
Several monolithic macroporous polymer sorbents (pore size 1–2 µm) based on alkyl methacrylates and ethylene glycol dimethacrylate as a cross-linking agent were prepared by free radical copolymerization in columns 3×150 mm. The influence of compositions of the reaction mixture and porogens and the nature of the alkyl radical in a mixture of monomers on the hydrodynamic and chromatographic characteristics of the monoliths was studied. The monoliths based on n-butyl methacrylate have rigid macroporous morphology and excellent hydrodynamic characteristics (flow rate up to 5 mL min−1). The efficiency of separation of a mixture of benzene and its derivatives in the version of reversed-phase HPLC was shown to increase with an increase in the fraction of a lauryl methacrylate additive (LMA) in the reaction mixture. The maximum separation efficiency (number of theoretical plates (tp)) was 35 000 tp m−1 for the monolith based on n-butyl methacrylate with 7% LMA in the reaction mixture.
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A. Yu. Kanat’eva, A. A. Kurganov, E. N. Viktorova, A. A. Korolev, Usp. Khim., 2008, 77, 393 [Russ. Chem. Rev., 2008, 77, 373 (Engl. Transl.)].
T. B. Tennikova, B. G. Belenkii, F. Svec, J. Liq. Chromatogr., 1990, 13, 63.
A. Maruška, A. Rocco, O. Kornyšova, J. Biochem. Biophys. Methods, 2007, 70, 47.
Monolithic Materials: Preparation, Properties and Applications, Eds F. Svec, T. B. Tennikova, Z. Deyl, Elsevier, Amsterdam, 2003, 67, 778 pp.
Khimicheskaya entsiklopediya [Chemical Encyclopedia], Ed. N. S. Zefirov, Bol’shaya Rossiiskaya Entsiklopediya, Moscow, 1995, Vol. 4, 642 (in Russian).
S. M. Matusova, L. N. Yazykova, K. I. Ivanova, A. V. Pirogov, O. A. Shpigun, Tez. dokl. Vseros. simp. “Khromatografiya v khimicheskom analize i fiziko-khimicheskikh issledovaniyakh” [Proc. All-Russia Symp. “Chromatography in Chemical Analysis and Physicochemical Studies”] (Moscow-Klyaz’ma, April 23–27, 2007), Moscow, 2007, 74 (in Russian).
S. M. Matusova, A. V. Pirogov, T. B. Tennikova, O. A. Shpigun, Zh. Prikl. Khim., 2007, 80, 652 [J. Appl. Chem., 2007, 80, 638 (Engl. Transl.)].
G. N. Khimich, E. N. Rakhmatullina, M. Yu. Slabospitskaya, T. B. Tennikova, Zh. Prikl. Khim., 2005, 78, 623 [J. Appl. Chem., 2005, 78, 617 (Engl. Transl.)].
S. N. Lanin, Doct. Sci. (Chem.) Thesis, Moscow State University, Moscow, 1998, 256 pp. (in Russian).
S. N. Lanin, Yu. S. Nikitin, Zh. Anal. Khim., 1991, 46, 1493 [J. Anal. Chem. USSR, 1991, 46, 1475 (Engl. Transl.)].
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Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 12, pp. 2503–2509, December, 2008.
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Matusova, S.M., Ivanova, K.I., D’yachkov, I.A. et al. Poly(alkyl methacrylate) monolithic columns for HPLC. Russ Chem Bull 57, 2554–2560 (2008). https://doi.org/10.1007/s11172-008-0367-2
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DOI: https://doi.org/10.1007/s11172-008-0367-2