Abstract
The main dynamic characteristics of biochemical methanol formation by the oxidation of methane using a biocatalyst were studied. The biocatalyst is based on cells of bacteria Methylosinus sporium B-2121, both suspended in a medium and immobilized in the poly(vinyl alcohol) cryogel. The change in the methane concentration and the biocatalyst amount affects the productivity of the system, the maximal concentration of methanol in the cultural liquid, and the rate of methanol accumulation. The most part of the dynamic characteristics are described by extremal curves. The experimental conditions were optimized prior to experiments. The use of the immobilized biocatalyst makes it possible to enhance the productivity of the process more than fivefold compared to that of the free cells and to achieve the highest methanol concentration in the medium: 62±2 mg L−1.
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Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 8, pp. 1603–1606, August, 2008.
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Razumovsky, S.D., Efremenko, E.N., Makhlis, T.A. et al. Effect of immobilization on the main dynamic characteristics of the enzymatic oxidation of methane to methanol by bacteria Methylosinus sporium B-2121. Russ Chem Bull 57, 1633–1636 (2008). https://doi.org/10.1007/s11172-008-0211-8
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DOI: https://doi.org/10.1007/s11172-008-0211-8