Abstract
Synthesis of a phosphoramidite reagent for the preparation of oligonucleotides labeled at the 5′-end with a fluorescent dye, 3,3,3′,3′-tetramethyl-2,2′-indodicarbocyanine, is described. The efficiency of this reagent is confirmed by the synthesis of several labeled oligonucleotides.
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References
V. V. Didenko, Biotechniques, 2001, 31, 1106.
D. Klostermeier and D. P. Millar, Biopolymers, 2002, 61, 159.
L. J. Kricka, Ann. Clin. Biochem., 2002, 39, 114.
A. Mishra, R. K. Behera, P. K. Behera, B. K. Mishra, and G. B. Behera, Chem. Rev., 2000, 100, 1973.
B. A. Armitage, Top. Curr. Chem., 2005, 253, 55.
S. Hohng, C. Joo, and T. Ha, Biophys. J., 2004, 87, 1328.
C. K. Brush and E. D. Anderson, US Pat. 5556959, 1996.
G. M. Little, R. Raghavachari, N. Narayanan, and H. L. Osterman, US Pat. 6027709, 2000.
A. S. Waggoner, US Pat. 6225050, 2001.
N. Narayanan, US Pat. 6593148, 2003.
G. Caputo and L. Della Ciana, US Pat. 6740755, 2004.
W. Bannwarth and A. Trzeciak, Helv. Chim. Acta, 1987, 70, 175.
E. A. Borisevich, V. N. Knyukshto, A. N. Kozyrev, and K. N. Solov’ev, Optika i Spektrosk., 1993, 74, 210 [Optics and Spectroscopy, 1993, 74, 129 (Engl. Transl.)].
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Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 1, pp. 154–158, January, 2006.
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Kvach, M.V., Gontarev, S.V., Prokhorenko, I.A. et al. Simple reagent for the synthesis of oligonucleotides labeled with 3,3,3′,3′-tetramethyl-2,2′-indodicarbocyanine. Russ Chem Bull 55, 159–163 (2006). https://doi.org/10.1007/s11172-006-0230-2
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DOI: https://doi.org/10.1007/s11172-006-0230-2