Skip to main content
SpringerLink
Log in

Universal method for SNP identification

  • Letters to the Editor
  • Published:
Russian Journal of Bioorganic Chemistry Aims and scope Submit manuscript

Abstract

A new approach to the identification of point mutations by allele-specific PCR was proposed. The mutation R408W of the human phenylalanine hydroxylase gene was used as a model. A high specificity of the approach was achieved by the use of primers partially complementary to the genomic DNA. Polyethylene glycol covalently attached to one of the allele-specific primers provides for the differential identification of the PCR products due to a change in electrophoretic mobility.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AS-PCR:

allele-specific PCR

AS-primer:

allele-specific primer

LNA-primer:

locked nucleic acid primer

REFERENCES

  1. Latorra, D., Campbell, K., Wolter, A., and Hurley, J., Human Mutation, 2003, vol. 22, pp. 79–85.

    Article  CAS  PubMed  Google Scholar 

  2. McKinzie, P.B. and Parsons, B.L., Mutation Res., 2002, vol. 517, pp. 209–220.

    CAS  PubMed  Google Scholar 

  3. Dutton, C. and Sommer, S.S., BioTechniques, 1991, vol. 11, pp. 700–702.

    CAS  PubMed  Google Scholar 

  4. Liu, Q., Thorland, E.C., Heit, J.A., and Sommer, S.S., Genome Res., 1997, vol. 7, pp. 389–398.

    CAS  PubMed  Google Scholar 

  5. Bonora, G.M., Ivanova, E., Zarytova, V., Burcovich, B., and Veronese, F.M., Bioconjugate Chem., 1997, vol. 8, pp. 793–797.

    Article  CAS  Google Scholar 

  6. Pyshnyi, D.V., Skobel’tsyna, L.M., Gushchina, E.N., Pyshnaya, I.A., Shishkina, I.G., Dymshits, G.M., Zarytova, V.F., and Ivanova, E.M., Mol. Biol. (Moscow), 2000, vol. 34, pp. 984–997.

    Article  CAS  Google Scholar 

  7. Smagulova, F.O. and Morozov, I.V., Bioorg. Khim., 2000, vol. 26, pp. 838–843.

    CAS  PubMed  Google Scholar 

  8. Patrushev, L.I., Zykova, E.S., Kayushin, A.L., Korosteleva, M.D., and Miroshnikov, A.I., Bioorg. Khim., 1998, vol. 24, pp. 194–200.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Novosibirsk State University, ul. Pirogova 2, Novosibirsk, 630090, Russia

    E. V. Brenner

  2. Institute of Chemical Biology and Fundamental Medicine, Siberian Division, Russian Academy of Sciences, pr. akademika Lavrent’eva 8, Novosibirsk, 630090, Russia

    E. V. Brenner, E. M. Ivanova, D. V. Pyshnyi & I. V. Morozov

Authors
  1. E. V. Brenner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. M. Ivanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. V. Pyshnyi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. V. Morozov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. V. Brenner.

Additional information

__________

Translated from Bioorganicheskaya Khimiya, Vol. 31, No. 2, 2005, pp. 213–215.

Original Russian Text Copyright © 2005 by Brenner, Ivanova, Pyshnyi, Morozov.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brenner, E.V., Ivanova, E.M., Pyshnyi, D.V. et al. Universal method for SNP identification. Russ J Bioorg Chem 31, 196–197 (2005). https://doi.org/10.1007/s11171-005-0027-3

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11171-005-0027-3

Key words

Search

Navigation