Abstract
We studied the possibility of optimizing the DNA transfection properties of carriers based on lysine dendrimers of the third and the fifth generation, including those containing a chloroacetyl or a lipophilic palmitoyl moiety at the C-end. The use of the lysosome-destroying antibiotic chloroquine and the amphipathic polycationic nonadecapeptide JTS-1 was found to enhance the DNA-transfecting properties of the lysine dendrimers. A triple complex including DNA, a lysine dendrimer of the third generation modified with lipophilic moieties of palmitic acid at its C-end, and JTS-1 was shown to be comparable in its transfecting activity to a complex containing Escort™, a commercial cationic liposome carrier.
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Abbreviations
- chloroquine:
-
N′-[(7-chloro-4-quinolyl)]-N,N-diethylpentane-1,4-diamine
- DABCO:
-
1,4-diazabicyclo[2.2.2]octane
- DAPI:
-
4,6-diamidino-2-phenylindole
- DIC:
-
diisopropylcarbodiimide
- DOPE:
-
dioleoylphosphatidylethanolamine
- DOTAP:
-
1,2-dioleoyloxy-3-(trimethylamino)propane
- Escort™:
-
a commercial liposomal carrier, a 3 : 1 DOTAP-DOPE mixture
- HOBt:
-
hydroxybenzotriazole
- α,ɛ-Lys:
-
Lys residue branched at α- and ɛ-amino groups
- Palm:
-
palmitic acid residue
- TNBS:
-
2,4,6-trinitrobenzenesulfonic acid
- X-gal:
-
5-bromo-4-chloro-3-indolyl D-galactopyranoside
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Translated from Bioorganicheskaya Khimiya, Vol. 31, No. 2, 2005, pp. 167–174.
Original Russian Text Copyright © 2005 by Vlasov, Lesina, Korol’kov, Gur’yanov, Bayanova, Baranov, Kiselev, Baranov.
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Vlasov, G.P., Lesina, E.A., Korol’kov, V.I. et al. Optimization of transfection properties of DNA-lysine dendrimer complexes. Russ J Bioorg Chem 31, 153–159 (2005). https://doi.org/10.1007/s11171-005-0021-9
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DOI: https://doi.org/10.1007/s11171-005-0021-9