Abstract
It was shown that the shuffling mechanism for transcribed genome components, which involves a template switch during the RNA reverse transcription using the L1 retroelement enzymatic machinery, is common in mammals. The occurrence frequency of the resulting chimeric retroelements in the genomes of rodents is twice as high as in the DNA of primates. Moreover, we proved that not only single but also double switches may occur in vivo, which result in the fusion of copies of three different transcripts. Many of the identified chimeras are transcribed in mammals.
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Abbreviations
- EST:
-
expressed sequence tag
- LINE:
-
long interspersed nuclear elements
- ORF:
-
open reading frame
- SINE:
-
short interspersed nuclear elements
- snRNA:
-
small nuclear RNA
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Translated from Bioorganicheskaya Khimiya, Vol. 31, No. 1, 2005, pp. 82–89.
Original Russian Text Copyright © 2005 by Gogvadze, Buzdin, Sverdlov.
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Gogvadze, E.V., Buzdin, A.A. & Sverdlov, E.D. Multiple template switches on LINE-directed reverse transcription: The most probable formation mechanism for the double and triple chimeric retroelements in mammals. Russ J Bioorg Chem 31, 74–81 (2005). https://doi.org/10.1007/s11171-005-0010-z
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DOI: https://doi.org/10.1007/s11171-005-0010-z