Abstract
The cDNAs encoding the genes of new proteins, homologous to the well-known Green Fluorescent Protein (GFP) from the hydroid jellyfish Aequorea victoria, were cloned. Two green fluorescent proteins from one unidentified anthomedusa, a yellow fluorescent protein from Phialidium sp., and a nonfluorescent chromoprotein from another unidentified anthomedusa were characterized. Thus, a broad diversity of GFP-like proteins among the organisms of the class Hydrozoa in both spectral properties and primary structure was shown.
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Abbreviations
- GFP:
-
green fluorescent protein
REFERENCES
Lippincott-Schwartz, J. and Patterson, G.H., Science, 2003, vol. 300, pp. 87–91.
Johnson, F.H., Shimomura, O., Saiga, Y., Gershman, L.C., Reynolds, G.T., and Waters, J.R., J. Cell. Comp. Physiol., 1962, vol. 60, pp. 85–104.
Chalfie, M., Photochem. Photobiol., 1995, vol. 62, pp. 651–656.
Prasher, D.C., Eckenrode, V.K., Ward, W.W., Prendergast, F.G., and Cormier, M.J., Gene, 1992, vol. 111, pp. 229–233.
Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W., and Prasher, D.C., Science, 1994, vol. 263, pp. 802–805.
Matz, M.V., Fradkov, A.F., Labas, Y.A., Savitsky, A.P., Zaraisky, A.G., Markelov, M.L., and Lukyanov, S.A., Nat. Biotechnol., 1999, vol. 17, pp. 969–973.
Labas, Y.A., Gurskaya, N.G., Yanushevich, Y.G., Fradkov, A.F., Lukyanov, K.A., Lukyanov, S.A., and Matz, M.V., Proc. Natl. Acad. Sci. USA, 2002, vol. 99, pp. 4256–4261.
Xia, N.S., Luo, W.X., Zhang, J., Xie, X.Y., Yang, H.J., Li, S.W., Chen, M., and Ng, M.H., Mar. Biotechnol., 2002, vol. 4, pp. 155–162.
Gurskaya, N.G., Fradkov, A.F., Pounkova, N.I., Staroverov, D.B., Bulina, M.E., Yanushevich, Y.G., Labas, Y.A., Lukyanov, S., and Lukyanov, K.A., Biochem. J., 2003, vol. 373, pp. 403–408.
Brejc, K., Sixma, T.K., Kitts, P.A., Kain, S.R., Tsien, R.Y., Ormo, M., and Remington, S.J., Proc. Natl. Acad. Sci. USA, 1997, vol. 94, pp. 2306–2311.
Remington, S.J., Methods Enzymol., 2000, vol. 305, pp. 196–211.
Verkhusha, V.V. and Lukyanov, K.A., Nat. Biotechnol., 2004, vol. 22, pp. 289–296.
Cormack, B.P., Valdivia, R.H., and Falkow, S., Gene, 1996, vol. 173, pp. 33–38.
Heim, R., Cubitt, A.B., and Tsien, R.Y., Nature, 1995, vol. 373, pp. 663–664.
Ormo, M., Cubitt, A.B., Kallio, K., Gross, L.A., Tsien, R.Y., and Remington, S.J., Science, 1996, vol. 273, pp. 1392–1395.
Salih, A., Larkum, A., Cox, G., Kuhl, M., and Hoegh-Guldberg, O., Nature, 2000, vol. 408, pp. 850–853.
Burr, A.H., Hunt, P., Wagar, D.R., Dewilde, S., Blaxter, M.L., Vanfleteren, J.R., and Moens, L., J. Biol. Chem., 2000, vol. 275, pp. 4810–4815.
Hochuli, E., Dobeli, H., and Schacher, A., J. Chromatogr., 1987, vol. 411, pp. 177–184.
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Translated from Bioorganicheskaya Khimiya, Vol. 31, No. 1, 2005, pp. 49–53.
Original Russian Text Copyright © 2005 by Yanushevich, Shagin, Fradkov, Shakhbazov, Barsova, Gurskaya, Labas, Matz, K. Lukyanov, S. Lukyanov.
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Yanushevich, Y.G., Shagin, D.A., Fradkov, A.F. et al. Spectral diversity among members of the green fluorescent protein family in hydroid jellyfish (Cnidaria, Hydrozoa). Russ J Bioorg Chem 31, 43–47 (2005). https://doi.org/10.1007/s11171-005-0005-9
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DOI: https://doi.org/10.1007/s11171-005-0005-9