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The hydroxyapatite nanowires as high loading carrier for immobilization of dextranase by crosslinking method

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Abstract

In this work, dextranase was immobilized on the surface of hydroxyapatite nanowires (HAPNWs) through covalent cross-linking method. The prepared HAPNWs-NH2-dextranase was characterized by FE-SEM, XRD, TGA and FT-IR spectroscopy. The results show that the dextranase was successfully immobilized on HAPNWs by covalent cross-linking method. The properties of HAPNWs-NH2-dextranase were studied and compared with free dextranase, such as catalytic activity, kinetic constants, thermal stability and pH stability. The loading capacity, storage stability and reusability of the immobilized enzymes were investigated. HAPNWs-NH2-dextranase demonstrated better enzyme activity at higher temperatures (92.28% vs 33.08% at 70 °C) and higher pH stability (93.17% vs 57.78% at pH 7.0) than free dextranase. The loading capacity of dextranase on HAPNWs-NH2-dextranase can reach up to 868.2 mg g−1. HAPNWs-NH2-dextranase retained 83.38% of its initial activity after 12 cycles, indicating that the HAPNWs-NH2-dextranase could have great performance in industry fields.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21707034), Natural Science Foundation of Educational Department of Anhui Province (No. KJ2020A0856), the National Key R&D Program of China (No. 2021YFF0701900), and the Science and Technology Commission of Shanghai Municipality (No. 20142200900).

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  1. Bingnan Sun and Yajie Wang have contributed equally to this work.

Authors and Affiliations

  1. Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Bingnan Sun, Haoran Yu, Lei Zhang & Weibing Zhang

  2. Department of Pharmacy, Anhui Medical College, Hefei, 230601, People’s Republic of China

    Yajie Wang

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  1. Bingnan Sun
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Contributions

BS and YW contributed equally to this work. BS: Validation, investigation, data curation, formal analysis, writing—original draft. YW: Investigation, formal analysis, conceptualization, funding acquisition. HY: Investigation. L: Conceptualization, supervision, methodology. WZ: Conceptualization, project administration, funding acquisition, supervision, methodology, writing—review and editing.

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Correspondence to Lei Zhang or Weibing Zhang.

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Sun, B., Wang, Y., Yu, H. et al. The hydroxyapatite nanowires as high loading carrier for immobilization of dextranase by crosslinking method. Res Chem Intermed 49, 4009–4024 (2023). https://doi.org/10.1007/s11164-023-05066-8

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