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Biological synthesis and characterization of gold nanoparticles using Verbascum speciosum Schrad. and cytotoxicity properties toward HepG2 cancer cell line

Abstract

Gold nanoparticles (AuNPs) are considered as one of the best modes of drug releasing plans for the treatment of various diseases. AuNPs have different applications in medical fields. The present project was conducted to synthesis of AuNPs by an easily green method by Verbascum speciosum Schrad. and to evaluate their antimicrobial effects and anti-proliferative activity against hepatocellular carcinoma cell line (HepG2). The produced nanoparticles were evaluated using UV–Vis spectroscopy, fourier-transform infrared spectroscopy (FT IR), field emission scanning electron microscopy (FESEM), and transmission electron microscopy (TEM). UV–Vis studies confirmed the formation of AuNPs and TEM images showed that the size of green synthesized AuNPs was about 118 ± 72 nm. Anti-proliferative activity against hepatocellular carcinoma cells (HepG2) using MTT assay showed that synthesized AuNPs inhibited the proliferation of cancer cells in time and concentration dependent manner. As the results showed, green synthesized AuNPs is effective in inhibiting the growth of common pathogenic bacteria and HepG2 cancer cell lines.

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References

  1. 1.

    AM Pillai VS Sivasankarapillai A Rahdar J Joseph F Sadeghfar K Rajesh GZ Kyzas 2020 J. Mol. Struct. 128107

  2. 2.

    A.E. Aferni, M. Guettari, T. Tajouri, A. Rahdar, J. Mol. Struct. 318, 114012 (2020)

    CAS  Google Scholar 

  3. 3.

    S.M. Hosseinikhah, M. Barani, A. Rahdar, H. Madry, R. Arshad, V. Mohammadzadeh, M. Cucchiarini, Mol. Sci 22, 3092 (2021)

    CAS  Article  Google Scholar 

  4. 4.

    M.E.T. Yazdi, F. Nourbakhsh, M. Mashreghi, S.H. Mousavi, Res. Chem. Intermed. 47, 1285 (2021)

    Article  CAS  Google Scholar 

  5. 5.

    M. Modarres, M.E.T. Yazdi, Iran J. Sci. Technol, Trans. A Sci. 45, 849 (2021)

    Article  Google Scholar 

  6. 6.

    M. Farhangi, Eshaghi, A., Taghavizadeh Yazdi, ME., Rahdar, A., Baino, F., J. Func. Biomat. (2021).

  7. 7.

    M.E.T. Yazdi, M.S. Amiri, H.A. Hosseini, R.K. Oskuee, H. Mosawee, K. Pakravanan, M. Darroudi, Bull. Mater. Sci. 42, 155 (2019)

    Article  CAS  Google Scholar 

  8. 8.

    A. Toro-Córdova, B. Sanz, G.F. Goya, Curr. Nanosci. 16, 399 (2020)

    Article  CAS  Google Scholar 

  9. 9.

    M.E.T. Yazdi, J. Khara, H.R. Sadeghnia, S.E. Bahabadi, M. Darroudi, Res. Chem. Intermed. 44, 1325 (2018)

    Article  CAS  Google Scholar 

  10. 10.

    M.E.T. Yazdi, J. Khara, M.R. Housaindokht, H.R. Sadeghnia, S.E. Bahabadi, M.S. Amiri, H. Mosawee, D. Taherzadeh, M. Darroudi, IET Nanobiotech. 13, 189 (2018)

    Article  Google Scholar 

  11. 11.

    S. Sasidharan, S. Raj, S. Sonawane, S. Sonawane, D. Pinjari, A. Pandit, P. Saudagar (2019). Elsevier.

  12. 12.

    K.X. Lee, K. Shameli, Y.P. Yew, S.-Y. Teow, H. Jahangirian, R. Rafiee-Moghaddam, T.J. Webster, Inter J. Nanomed. 15, 275 (2020)

    CAS  Article  Google Scholar 

  13. 13.

    M.E. Taghavizadeh Yazdi, A. Hamidi, M.S. Amiri, R. Kazemi Oskuee, H.A. Hosseini, A. Hashemzadeh, M. Darroudi (2019). Mater. Technol. 34, 490.

  14. 14.

    M.R. Hashemzadeh, M.E.T. Yazdi, M.S. Amiri, S.H. Mousavi, (2021). Tissue Cell. 101504.

  15. 15.

    A. Es-haghi, M.E. Taghavizadeh Yazdi, M. Sharifalhoseini, M. Baghani, E. Yousefi, A. Rahdar, F. Baino (2021). Biomimetics. 6, 34.

  16. 16.

    S. AseydNezhad, A. Es-haghi, M.H. Tabrizi, Appl. Organomet. Chem. 34, e5314 (2020)

    CAS  Google Scholar 

  17. 17.

    M.E.T. Yazdi, M. Modarres, M.S. Amiri, M. Darroudi, Res. Chem. Intermed. 45, 1105 (2019)

    Article  CAS  Google Scholar 

  18. 18.

    M.S. Amiri, V. Mohammadzadeh, M.E.T. Yazdi, M. Barani, A. Rahdar, G.Z. Kyzas, Molecules 26, 1770 (2021)

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  19. 19.

    M. Darroudi, M.E.T. Yazdi, M.S. Amiri, 21st Century Nanoscience–A Handbook (CRC Press, 2020)

    Google Scholar 

  20. 20.

    M.E.T. Yazdi, M. Darroudi, M.S. Amiri, H.A. Hosseini, F. Nourbakhsh, M. Mashreghi, M. Farjadi, S.M.M. Kouhi, S.H. Mousavi, Micro Nano Letters 15, 1046 (2020)

    CAS  Article  Google Scholar 

  21. 21.

    A Rana K Yadav S Jagadevan 2020 J. Cleaner Prod. 122880

  22. 22.

    H. Sharifan, J. Moore, X. Ma, Ecotoxicol. Environ. Saf. 191, 110177 (2020)

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  23. 23.

    M.E.T. Yazdi, M.S. Amiri, S. Akbari, M. Sharifalhoseini, F. Nourbakhsh, M. Mashreghi, M.R. Abbasi, M. Modarres, A. Es-haghi, BioNanoSci. 10, 1121 (2020)

    Article  Google Scholar 

  24. 24.

    M. Ashna, A. Es-Haghi, M. Karimi Noghondar, D. Al Amara, M.E.T. Yazdi, (2020). Materials Technol.

  25. 25.

    M.E. Taghavizadeh Yazdi, S. Nazarnezhad, S.H. Mousavi, M. Sadegh Amiri, M. Darroudi, F. Baino, S. Kargozar (2020). Molecules. 26, 1510.

  26. 26.

    M.R. Chitsazi, H. Korbekandi, G. Asghari, R. Bahri Najafi, A. Badii, S. Iravani (2016). Artif. cells Nanomed. Biotechnol. 44, 328.

  27. 27.

    A.B.-K. Seyed Mousa Mousavi-kouhi, Mohammad Sadegh Amiri, Mohammad Mashreghi, Mohammad Ehsan Taghavizadeh Yazdi (2021). Ceram Int. 47, 214.

  28. 28.

    M. Zarei, E. Karimi, E. Oskoueian, A. Es-Haghi, M.E.T. Yazdi, Nutrition Cancer 73, 1511 (2020)

    PubMed  Article  CAS  PubMed Central  Google Scholar 

  29. 29.

    M.S. Amiri, M.E.T. Yazdi, M. Rahnama, Plant Sci. Today 8, 95 (2021)

    Article  CAS  Google Scholar 

  30. 30.

    T. Yazdi, M. Ehsan, M.R. Housaindokht, H.R. Sadeghnia, S. Esmaeilzadeh Bahabadi, M.S. Amiri, M. Darroudi (2020). Stud. Med. Sci. 31, 75.

  31. 31.

    P.M. Dey, J.B. Harborne, Plant biochemistry, Elsevier1997.

  32. 32.

    F. Mobaraki, M. Momeni, M.E.T. Yazdi, Z. Meshkat, M.S. Toosi, S.M. Hosseini, Process Biochem. 111, 167 (2021)

    CAS  Article  Google Scholar 

  33. 33.

    E. Rodríguez-León, B.E. Rodríguez-Vázquez, A. Martínez-Higuera, C. Rodríguez-Beas, E. Larios-Rodríguez, R.E. Navarro, R. López-EsparzaR.A., Nanoscale Res. Lett. 14, 1 (2019)

    Article  CAS  Google Scholar 

  34. 34.

    G. Elango, S.M. Roopan, Spectrochim. Acta Part A. 139, 367 (2015)

    CAS  Article  Google Scholar 

  35. 35.

    T. Surendra, S.M. Roopan, N.A. Al-Dhabi, M.V. Arasu, G. Sarkar, K. Suthindhiran, Nano. Res. lett. 111, 10 (2016)

    Google Scholar 

  36. 36.

    S.M.R. Devipriya, Mater. Sci. Eng. C. 80, 38 (2017)

    CAS  Article  Google Scholar 

  37. 37.

    Balasubramanian, L. Yang, L.-Y.L. Yung, C.-N. Ong, W.-Y. Ong, E.Y. Liya (2010), Biomat. 31, 9023.

  38. 38.

    S.A. Akintelu, B. Yao, A.S. Folorunso, Plasmonics 16, 157 (2021)

    CAS  Article  Google Scholar 

  39. 39.

    U. Ullah, A. Rauf, E. El-Sharkawy, F.A. Khan, A. Khan, S.M. Bukhari, S. Bawazeer, Y.N. Mabkhot, B.K. Malikovna, G. Kazhybayeva, Bioprocess Biosyst. Eng. 44, 1185 (2021)

    CAS  PubMed  Article  Google Scholar 

  40. 40.

    Y. Guo, N. Jiang, L. Zhang, M. Yin, Arab J. Chem. 13, 5096 (2020)

    CAS  Article  Google Scholar 

  41. 41.

    T. Zhang, M. Dang, W. Zhang, X. Lin, J. Photochem. Photobiol. B: Biol. 202, 111705 (2020)

    CAS  Article  Google Scholar 

  42. 42.

    Y. Manetas, New Phytol. 158, 503 (2003)

    Article  Google Scholar 

  43. 43.

    S. Kayır, Y. Demirci, S. Demirci, E. Ertürk, E. Ayaz, A. Doğan, F. Şahin, i (2018). S. Afr. J. Bot. 119, 226.

  44. 44.

    K. Nofouzi, R. Mahmudi, K. Tahapour, E. Amini, K. Yousefi, J. Essent. Oil-Bear. Plants. 19, 499 (2016)

    CAS  Article  Google Scholar 

  45. 45.

    R. Karamian, F. Ghasenlou, Study of saponin content in aerial parts and roots of three Verbascum L. species (2015). J. Plant Res. (Iranian J. Biol.), 28, 596.

  46. 46.

    M. Halimi, M. Nasrabadi, Q. J. Iran Chem. Commun. 6, 143 (2018)

    Google Scholar 

  47. 47.

    H. Vahedi, J. Lari, M. Halimi, M. Nasrabadi, A. Vahedi, J. Essent. Oil-Bear. Plants. 15, 895 (2012)

    Article  CAS  Google Scholar 

  48. 48.

    Q.D. Do, A.E. Angkawijaya, P.L. Tran-Nguyen, L.H. Huynh, F.E. Soetaredjo, S. Ismadji, Y.-H. Ju, J. Food Drug Analys. 22, 296 (2014)

    CAS  Article  Google Scholar 

  49. 49.

    P. Kuppusamy, M.M. Yusoff, N.R. Parine, N. Govindan, Saudi J. Biol. Sci. 22, 293 (2015)

    PubMed  Article  PubMed Central  Google Scholar 

  50. 50.

    D.-H. Truong, D.H. Nguyen, N.T.A. Ta, A.V. Bui, T.H. Do, H.C. Nguyen, J. Food Qual. (2019). https://doi.org/10.1155/2019/8178294

    Article  Google Scholar 

  51. 51.

    J. Martınez, N. Chequer, J. González, T. Cordova, Nanosci. Nanotechnol. 2, 184 (2012)

    Article  CAS  Google Scholar 

  52. 52.

    M. Riaz, M. Zia-Ul-Haq, H.Z. Jaafar, Rev. Bras. Farmacogn. 23, 948 (2013)

    CAS  Article  Google Scholar 

  53. 53.

    K. Thumanu, M. Sompong, P. Phansak, K. Nontapot, N. Buensanteai, J. Plant Interact. 10, 270 (2015)

    CAS  Article  Google Scholar 

  54. 54.

    R.-B. Xu, X. Yang, J. Wang, H.-T. Zhao, W.-H. Lu, J. Cui, C.-L. Cheng, P. Zou, W.-W. Huang, P. Wang, Int. J. Mol. Sci. 13, 14262 (2012)

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  55. 55.

    W.D. Pyrz, D.J. Buttrey, Langmuir 24, 11350 (2008)

    CAS  PubMed  Article  Google Scholar 

  56. 56.

    Z. Shamasi, A. Es-haghi, M.E. TaghavizadehYazdi, M.S. Amiri, M. Homayouni-Tabrizi, Nanomed. J. 8, 65 (2020)

    Google Scholar 

  57. 57.

    X. Liu, K. Shan, X. Shao, X. Shi, Y. He, Z. Liu, J.A. Jacob, L. Deng, Int. J. Nanomed. 16, 753 (2021)

    Article  Google Scholar 

  58. 58.

    S. Ghiasvand, M.A. Javidi, A. Mohammadian, S.A. Mousavi, F. Shahriari, F. Alavian, Life Sci. 266, 118874 (2021)

    CAS  PubMed  Article  Google Scholar 

  59. 59.

    M.E. TaghavizadehYazdi, M. Darroudi, M.S. Amiri, H. Zarrinfar, H.A. Hosseinie, M. Mashreghi, H. Mozafarri, A. Ghorbani, S.H. Mousavi, Iran J Sci Technol (Science, Transactions A, 2021)

    Google Scholar 

  60. 60.

    A.E. Ahmadian, S.M. Dizaj, E. Rahimpour, A. Hasanzadeh, A. Eftekhari, J. Halajzadeh, H. Ahmadian, Mater. Sci. Engin: C 93, 465 (2018)

    CAS  Article  Google Scholar 

  61. 61.

    A. Solhaug, M.L. Torgersen, J.A. Holme, J. Wiik-Nilsen, B. Thiede, G.S. Eriksen, Toxicology 433, 152405 (2020)

    PubMed  Article  CAS  Google Scholar 

  62. 62.

    L. Taiz, E. Zeiger, I.M. Møller, A. Murphy, (2015). Plant physiology and development, Sinauer Associates Incorporated.

  63. 63.

    A. Campu, M. Focsan, F. Lerouge, R. Borlan, L. Tie, D. Rugina, S. Astilean, Colloids Surf. B. 194, 111213 (2020)

    CAS  Article  Google Scholar 

  64. 64.

    E.C. Dreaden, L.A. Austin, M.A. Mackey, M.A. El-Sayed, Ther. Deliv. 3, 457 (2012)

    CAS  PubMed  Article  Google Scholar 

  65. 65.

    S. Rajeshkumar, M. Sherif, C. Malarkodi, M. Ponnanikajamideen, M.V. Arasu, N.A. Al-Dhabi, S.M. Roopan, J. Mol. Struct. 1228, 129440 (2021)

    CAS  Article  Google Scholar 

  66. 66.

    S. Rajeshkumar, C. Malarkodi, D.A. Al Farraj, M.S. Elshikh, S.M. Roopan, Mater. Today Commun. 26, 101975 (2021)

    CAS  Google Scholar 

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Acknowledgements

The authors would like to thank University of Birjand and Mashhad University of Medical Sciences for providing facilities of this work.

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Mousavi-Kouhi, S.M., Beyk-Khormizi, A., Mohammadzadeh, V. et al. Biological synthesis and characterization of gold nanoparticles using Verbascum speciosum Schrad. and cytotoxicity properties toward HepG2 cancer cell line. Res Chem Intermed (2021). https://doi.org/10.1007/s11164-021-04600-w

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Keywords

  • Verbascum speciosum Schrad.
  • AuNPs
  • Human liver cancer cell line (HepG2)
  • Scrophulariaceae