Biosynthesis of gold nanoparticles using Prosopis farcta extract and its in vitro toxicity on colon cancer cells
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Gold nanoparticles (Au-NPs) were prepared by “green” synthesis through the aqueous extract of Prosopis farcta. The P. farcta is a special herbal, which it can be a suitable candidate in the reduction process of Au3+ to Au0. The structure and morphology of the synthesized Au-NPs were characterized by UV–visible spectrophotometry, powder X-ray diffraction analysis and high-magnification transmission electron microscopy techniques. The Au-NPs were spherically uniform in shape with particle size of about 25 nm. The cytotoxicity effect of Au-NPs was studied on a colon (HT-29) cancerous cell line via the MTT assay, and the IC50 of Au-NPs was calculated to be 419.7 µg/mL. The results indicated that the apoptotic effects were increased on cells when the Au-NPs concentration reached 200 µg/mL. The Au-NPs were synthesized easily and with a very low cost and time by P. farcta extract for the first time.
KeywordsGold nanoparticles Prosopis farcta Cytotoxicity Apoptotic TUNEL assay
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 7.A. Miri, N. Dorani, M. Darroudi, M. Sarani, Cell. Mol. Biol. 62, 46 (2016)Google Scholar
- 13.F. Harzallah-Skhiri, H. BenJannet, J. Appl. Sci. Res. 1, 130 (2005)Google Scholar
- 15.S. Al-Qurán, J. Nat. Prod. 1, 10 (2008)Google Scholar
- 19.A. Tomar, G. Garg, Global J. Pharm. 7, 34 (2013)Google Scholar