Research on Chemical Intermediates

, Volume 44, Issue 5, pp 3169–3177 | Cite as

Biosynthesis of gold nanoparticles using Prosopis farcta extract and its in vitro toxicity on colon cancer cells

  • Abdolhossien Miri
  • Majid Darroudi
  • Reza Entezari
  • Mina SaraniEmail author


Gold nanoparticles (Au-NPs) were prepared by “green” synthesis through the aqueous extract of Prosopis farcta. The P. farcta is a special herbal, which it can be a suitable candidate in the reduction process of Au3+ to Au0. The structure and morphology of the synthesized Au-NPs were characterized by UV–visible spectrophotometry, powder X-ray diffraction analysis and high-magnification transmission electron microscopy techniques. The Au-NPs were spherically uniform in shape with particle size of about 25 nm. The cytotoxicity effect of Au-NPs was studied on a colon (HT-29) cancerous cell line via the MTT assay, and the IC50 of Au-NPs was calculated to be 419.7 µg/mL. The results indicated that the apoptotic effects were increased on cells when the Au-NPs concentration reached 200 µg/mL. The Au-NPs were synthesized easily and with a very low cost and time by P. farcta extract for the first time.


Gold nanoparticles Prosopis farcta Cytotoxicity Apoptotic TUNEL assay 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Abdolhossien Miri
    • 1
  • Majid Darroudi
    • 2
  • Reza Entezari
    • 3
  • Mina Sarani
    • 4
    Email author
  1. 1.Department of Pharmacognosy, Faculty of PharmacyZabol University of Medical SciencesZabolIran
  2. 2.Nuclear Medicine Research CenterMashhad University of Medical SciencesMashhadIran
  3. 3.Department of Pharmaceutical Biotechnology, Faculty of PharmacyZabol University of Medical ScienceZabolIran
  4. 4.Zabol Medicinal Plants Research CenterZabol University of Medical SciencesZabolIran

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