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Free radical scavenging and antidiabetic activities of Euonymus laxiflorus Champ. extract

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Abstract

In recent years, due to the undesirable side-effects of synthetic drugs used to treat diabetes, research into herbal medicines for treatment of this disease has increased. Euonymus laxiflorus Champ. belongs to the Celastraceae family, long used in traditional Vietnamese medicine by ethnic people living in Dak Lak Province of Vietnam. The aim of this study is to evaluate the antioxidant and antidiabetic activities of methanol extract of Euonymus laxiflorus Champ. trunk bark. The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and the antidiabetic activity was determined by inhibition of digestive enzymes and lowering of fasting blood glucose in diabetic rats. The contents of flavonoids and polyphenols were also determined spectroscopically. The results indicated that trunk bark extract of Euonymus laxiflorus Champ. possessed relatively high antioxidant activity with 50% inhibition concentration (IC50) of 0.60 mg/ml, compared with 0.24 mg/ml for l-ascorbic acid as positive control. Furthermore, the extract also exhibited relatively higher α-glucosidase inhibitory and moderately lower α-amylase inhibitory effects compared with acarbose. Moreover, the extract could lower fasting blood glucose levels in rats with streptozotocin-induced diabetes but without inducing body weight loss, compared with acarbose. However, the extract did not produce any hypoglycemic effect in normal rats at dose up to 300 mg/kg body weight. These results suggest that methanol extract of Euonymus laxiflorus Champ. may prove to be useful in treating type 2 diabetes.

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References

  1. S.A. Ross, E.A. Gulve, M. Wang, Chem. Rev. 104, 1255 (2004)

    Article  CAS  Google Scholar 

  2. R. Klein, B.E. Klein, S.E. Moss, K.J. Cruickshanks, Intern. Med. 154, 2169 (1994)

    CAS  Google Scholar 

  3. C.J.A. Abraira Colwell, F.Q. Nuttall, C.T. Sawin, N.J. Nagel, J.P. Comstock, N.V. Emanuele, S.R. Levin, W. Henderson, H.S. Lee, Diabetes Care 18, 1113 (1995)

    Article  Google Scholar 

  4. Y. Ohkubo, H. Kishikawa, E. Araki, T. Miyata, S. Isami, S. Motoyoshi, Y. Kojima, N. Furuyoshi, M. Shichiri, Diabetes Res. Clin. Pract. 28, 103 (1995)

    Article  CAS  Google Scholar 

  5. The Diabetes Control and Complications Trial Research Group, N. Engl. J. Med. 329, 977 (1993)

    Article  Google Scholar 

  6. R.A. De-Fronzo, Ann. Intern. Med. 131, 281 (1999)

    Article  CAS  Google Scholar 

  7. A.J. Scheen, N. Paquot, Diabetes Metab. 39, 179 (2013)

    Article  CAS  Google Scholar 

  8. P. Mukherjee, K. Maiti, K. Mukherjee, P.J. Houghton, J. Ethnopharmacol. 106, 1 (2006)

    Article  CAS  Google Scholar 

  9. A. Tiwari, J. Rao, Curr. Sci. 83, 30 (2002)

    CAS  Google Scholar 

  10. L.M. Liu, S.F. Cheng, P.C. Shieh, J.C. Lee, J.J. Chen, C.T. Ho, S.C. Kuo, D.H. Kuo, L.J. Huang, T.D. Way, Food Chem. Toxicol. 70, 179 (2014)

    Article  CAS  Google Scholar 

  11. H.S. Lee, S.H. Jung, B.S. Yun, K.W. Lee, Arch. Toxicol. 81, 211 (2007)

    Article  CAS  Google Scholar 

  12. V.B. Nguyen, A.D. Nguyen, Q.V. Nguyen, S.L. Wang, Res. Chem. Intermed. 43, 259 (2017)

    Article  CAS  Google Scholar 

  13. G. Miliauskas, P.R. Venskutonis, T.A. Van Beek, Food Chem. 85, 231 (2004)

    Article  CAS  Google Scholar 

  14. O. Folin, V. Ciocalteu, J. Biol. Chem. 27, 627 (1927)

    Google Scholar 

  15. Z. Jia, M. Tang, J. Wu, Food Chem. 64, 555 (1999)

    Article  Google Scholar 

  16. K.W. Kim, S.J. Suh, J.D. Kim, S.S. Kim, I.S. Lee, J.K. Kim, G.T. Chang, D.S. Kim, C.H. Kim, Basic Clin. Pharmacol. Toxicol. 104, 60 (2008)

    Article  Google Scholar 

  17. Q.V. Nguyen, V.B. Nguyen, J.B. Eun, S.L. Wang, D.H. Nguyen, T.N. Tran, A.D. Nguyen, Res. Chem. Intermed. 42, 5859 (2016)

    Article  CAS  Google Scholar 

  18. OECD, OECD Guidelines for Testing of Chemicals 423: Acute Oral Toxicity-Acute Toxic Class Method (OECD, Paris, 2001)

    Google Scholar 

  19. S. Ramachandran, K. Asokkumar, M. Uma Maheswari, T.K. Ravi, A.T. Sivashanmugam, S. Saravanan, Evid. Based Complement. Altern. Med. 57, 17 (2011)

    Google Scholar 

  20. G.C. Yen, P.D. Duh, C.L. Tsai, J. Agric. Food Chem. 41, 67 (1993)

    Article  CAS  Google Scholar 

  21. K.B. Pandey, S.I. Rizvi, Curr. Nutr. Food Sci. 8, 254 (2012)

    Article  CAS  Google Scholar 

  22. P.K. Agraval, Carbon-13 NMR of flavonoids, vol. 39, (Elsevier, New York, 1989), p. 1

    Book  Google Scholar 

  23. D.M. Gray, N. Engl. J. Med. 29, 1225 (1995)

    Google Scholar 

  24. A.J. Krentz, C.J. Bailey, Drugs 65, 385 (2005)

    Article  CAS  Google Scholar 

  25. S.K. Lee, J.Y. Hwang, J.H. Song, J.R. Jo, M.J. Kim, M.E. Kim, J.I. Kim, Nutr. Res. Pract. 1, 184 (2007)

    Article  Google Scholar 

  26. S. Wen, B.P. Kota, G. Peng, G.Q. Li, J. Yamahara, B.D. Roufogalis, J. Ethnopharmacol. 99, 239 (2005)

    Article  Google Scholar 

  27. A.D. Mooradian, J.E. Thurman, Drugs 57, 19–29 (1999)

    Article  CAS  Google Scholar 

  28. Y. Kumarasamy, P.J. Cox, M. Jaspars, L. Nahar, C.C. Wilcock, S.D. Sarker, Fitoterapia 74, 305 (2003)

    Article  CAS  Google Scholar 

  29. H.M. Chang, P.P. But, Danshen in Pharmacology and Applications of Chinese Materia Medica, ed. by H.M. Chang, P. But (Singapore, World Scientific, 1986), pp. 255–268

    Chapter  Google Scholar 

  30. E. De Sousa, I.S. Leila Zanatta, T.B. Creczynski-Pasa, M.G. Pizzolatti, B. Szpoganicz, F.R.M. Barreto Silva, J. Nat. Prod. 6, 829 (2004)

    Article  Google Scholar 

  31. T. Hanamura, H. Toshihiko, K. Hirokazu, Biosci. Biotechnol. Biochem. 69, 280 (2005)

    Article  CAS  Google Scholar 

  32. T.T. Mai, N.N. Thu, P.G. Tien, N.V. Chuyen, J. Nutr. Sci. Vitaminol. (Tokyo) 53, 267 (2007)

    Article  CAS  Google Scholar 

  33. Y.I. Kwon, H.D. Jang, K. Shetty, Asia Pac. J. Clin. Nutr. 15, 425 (2006)

    Google Scholar 

  34. J.Y. Youn, H.Y. Park, K.H. Cho, Diabetes Res. Clin. Pract. 66S, S149 (2004)

    Article  Google Scholar 

  35. V. Vats, J.K. Grover, S.S. Rathi, J. Ethnopharmacol. 79, 95 (2002)

    Article  CAS  Google Scholar 

  36. P. Strobel, C. Allard, T. Perez-Acle, R. Calderon, R. Aldunate, F. Leighton, Biochem. J. 386, 471 (2005)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We would like to express our thanks to the Department of Science & Technology, Dak Lak Province, Vietnam (2550/QĐ.UBND 05.12.2013) for a grant to us to finish this work.

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Authors and Affiliations

  1. Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, Dak Lak Province, 630000, Vietnam

    Quang-Vinh Nguyen & Anh Dzung Nguyen

  2. Department of Chemistry, Tamkang University, New Taipei, 251, Taiwan

    San-Lang Wang & Van Bon Nguyen

  3. Department of Natural Science and Technology, Tay Nguyen University, Buon Ma Thuot, 630000, Vietnam

    Van Bon Nguyen

  4. Tay Nguyen Institute of Hygiene and Epidemiology, Buon Ma Thuot, 630000, Vietnam

    Ngoc-Hung Nguyen

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  1. Quang-Vinh Nguyen
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  2. Ngoc-Hung Nguyen
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  5. Anh Dzung Nguyen
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Correspondence to Quang-Vinh Nguyen, San-Lang Wang or Anh Dzung Nguyen.

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Nguyen, QV., Nguyen, NH., Wang, SL. et al. Free radical scavenging and antidiabetic activities of Euonymus laxiflorus Champ. extract. Res Chem Intermed 43, 5615–5624 (2017). https://doi.org/10.1007/s11164-017-2951-2

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