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Porcine pancreatic α-amylase inhibitors from Euonymus laxiflorus Champ.

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Abstract

Twenty-six samples of indigenous medicinal plants were collected in Dak Lak Province of Vietnam and evaluated for α-amylase inhibitory activity. Of these samples, trunk bark extract from Euonymus laxiflorus Champ. (ELC extract) showed the greatest α-amylase inhibitory activity with the smallest 50 % inhibitory concentration (IC50) of 6.6 ± 0.6 µg/mL against porcine pancreatic α-amylase. This extract possessed strong inhibitory activity against human saliva α-amylase and slightly lower activity against Bacillus subtilis α-amylase, with IC50 values of 4.2 ± 0.2 and 27.8 ± 1.8 µg/mL, respectively. ELC extract was found to be strongly thermostable, retaining 72–100 % relative activity even after heating at 100 °C for 5 min to 2 h. This extract was also stable at various pH values. These results suggest that ELC extract may be a good candidate for treatment of non-insulin-dependent diabetes mellitus (NIDDM) and obesity.

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Acknowledgments

This work was supported in part by a grant from the Ministry of Science and Technology, Taiwan (NSC 102-2313-B-032-001-MY3, NSC 103-2621-M-032-001) and by a grant from the Dak Lak Department of Science & Technology, Vietnam (2550/QĐ.UBND 05.12.2013).

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Authors and Affiliations

  1. Department of Chemistry, Tamkang University, 251, New Taipei, Taiwan

    Van Bon Nguyen & San-Lang Wang

  2. Institute of Biotechnology and Environment, Tay Nguyen University, 567 Le Duan Str., Buon Ma Thuot, Vietnam

    Anh Dzung Nguyen & Quang Vinh Nguyen

  3. Department of Science and Technology, Tay Nguyen University, 567 Le Duan Str., Buon Ma Thuot, Vietnam

    Van Bon Nguyen

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  1. Van Bon Nguyen
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  2. Anh Dzung Nguyen
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  4. San-Lang Wang
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Correspondence to San-Lang Wang.

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Nguyen, V.B., Nguyen, A.D., Nguyen, Q.V. et al. Porcine pancreatic α-amylase inhibitors from Euonymus laxiflorus Champ.. Res Chem Intermed 43, 259–269 (2017). https://doi.org/10.1007/s11164-016-2619-3

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  • DOI: https://doi.org/10.1007/s11164-016-2619-3

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