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Recognition of gram-negative and gram-positive bacteria with a functionalized conducting polymer film

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In this work, we successfully developed bacterial templates on the surface of an overoxidized polypyrrole film using both gram-negative and gram-positive bacteria in which bacterial surface chemical structures are precisely transferred at a molecular level. The sensor film identified target bacteria within minutes through a unique combination with dielectrophoresis. The bacterial cavities had high selectivity for distinguishing specific target bacteria in bacterial mixtures containing gram-negative (Escherichia coli and Pseudomonas aeruginosa) and gram-positive (Bacillus subtilis and Staphylococcus aureus) bacteria. This rapid and specific recognition system will enable not only bacterial sensing but also analysis of various biological species.

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We thank Prof. Yamazaki for arranging the use of the experimental facilities. We thank Dr. Okajima for assistance with bacterial cultivation. This work was partially supported by Special Coordination Funds for Promoting Scientific and Technology (SCF) of MEXT. We gratefully acknowledge the financial support provided by the Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (A) (grant 24685013), and the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries, and Food Industry (grant 25020A).

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Correspondence to Shiho Tokonami.

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Tokonami, S., Nakadoi, Y., Nakata, H. et al. Recognition of gram-negative and gram-positive bacteria with a functionalized conducting polymer film. Res Chem Intermed 40, 2327–2335 (2014).

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