Abstract
Human serum albumin (HSA) is the most abundant protein in human blood plasma. It is commonly used as a biomarker in urine samples to identify the chronic kidney disease caused by high blood pressure or diabetes. In our research, a thin layer of liquid crystals (LCs) is used as a readout system for developing an immunoassay that reports the presence of HSA in the aqueous solution with optical signals. The detection principle of this assay is based on the variation of surface density of protein upon the specific binding of HSA on an anti-HSA immobilized surface, which leads a dark-to-bright transition of LC images under cross-polarizers. Our results show that the LC-based immunoassay can detect HSA at a concentration of 50 μg/mL. By using the slide with immobilized anti-HSA in array format, the concentration level of HSA can be simply determined by the number of LC spot shown on the slide.
Similar content being viewed by others
References
R.G. Fassett, S.K. Venuthurupalli, G.C. Gobe, J.S. Coombes, M.A. Cooper, W.E. Hoy, Kidney Int. 80, 806 (2011)
P. Devarajan, Adv. Chronic Kidney Dis. 17, 469–479 (2010)
F.M. van der Kloet, F.W.A. Tempels, N. Ismail, R. van der Heijden, P.T. Kasper, M. Rojas-Cherto et al., Metabolomics 8, 109 (2012)
J.K. Snell-Bergeon, D.M. Maahs, L.G. Ogden, G.L. Kinney, J.E. Hokanson, E. Schiffer et al., Diabetes Technol. Ther. 11, 1 (2009)
C. Giovannoli, L. Anfossi, C. Baggiani, G. Giraudi, J. Chromatogr. A 1155, 187 (2007)
J. Benesch, A. Askendal, P. Tengvall, Colloids Surf. B 18, 71 (2000)
M.C. Tu, Y.T. Chang, Y.T. Kang, H.Y. Chang, P. Chang, T.R. Yew, Biosens. Bioelectron. 34, 286 (2012)
T. Uda, R. Kanmei, S. Akasofu, E. Hifumi, M. Ohtaki, Anal. Biochem. 218, 259 (1994)
W. Kashif, N. Siddiqi, A.P. Dincer, H.E. Dincer, S. Hirsch, Clevel. Clin. J. Med. 70, 535 (2003)
V.J. Alino, K.-L. Yang, Analyst 136, 3307 (2011)
C.Y. Xue, K.-L. Yang, J. Colloid Interface Sci. 344, 48 (2010)
C.-H. Chen, K.-L. Yang, Langmuir 27, 5427 (2011)
I.M. Vlasova, A.M. Saletsky, J. Appl. Spectrosc. 76, 536 (2009)
Acknowledgments
The funding of this work is supported by National Science Council (101-2113-M-032-007-MY2). We would like to thank Prof. Masakazu Anpo (OPU) and Prof. San-Lang Wang (TKU) for organizing the special issue for the First OPU-TKU International Symposium.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ho, WH., Chen, CH. Liquid crystal-based immunoassay for detecting human serum albumin. Res Chem Intermed 40, 2229–2236 (2014). https://doi.org/10.1007/s11164-014-1600-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11164-014-1600-2